Next Article in Journal
New Insights into Immunological Involvement in Congenital Disorders of Glycosylation (CDG) from a People-Centric Approach
Next Article in Special Issue
Impact of Advanced HIV Disease on Quality of Life and Mortality in the Era of Combined Antiretroviral Treatment
Previous Article in Journal
Estimated Population Prevalence of Heart Failure with Reduced Ejection Fraction in Spain, According to DAPA-HF Study Criteria
Open AccessArticle

Hepatitis C Virus Influences HIV-1 Viral Splicing in Coinfected Patients

1
Laboratory of Reference and Research on Viral Hepatitis, National Center of Microbiology, Institute of Health Carlos III, 28220 Majadahonda, Madrid, Spain
2
AIDS Immunopathology Unit, National Center of Microbiology, Institute of Health Carlos III, 28220 Majadahonda, Madrid, Spain
3
Servicio de Enfermedades Infecciosas, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS) and Hospital Universitario Ramón y Cajal, 28034 Madrid, Spain
4
Department of Immunology, Centro Nacional de Microbiología, Instituto de Salud Carlos III (ISCIII), 28220 Majadahonda, Madrid, Spain
5
Department of Infectious Diseases, Instituto de Investigación Sanitaria Hospital de la Paz (IdiPAZ), 28046 Madrid, Spain
6
Unidad VIH, Servicio de Medicina Interna, Instituto de Investigación Biomédica del Hospital Doce de Octubre (imas12), 28041 Madrid, Spain
7
Department of Infectious Diseases, Infanta Leonor Hospital, 28031 Madrid, Spain
8
Servicio de Medicina Interna-Infecciosas, Hospital Universitario de La Princesa, 28006 Madrid, Spain
9
Servicio de Medicina Interna, Hospital Puerta de Hierro, 28222 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Two last authors equally contributed to the manuscript.
The members can be seen in Acknowledgments part.
J. Clin. Med. 2020, 9(7), 2091; https://doi.org/10.3390/jcm9072091
Received: 24 June 2020 / Revised: 29 June 2020 / Accepted: 29 June 2020 / Published: 3 July 2020
Coinfection with hepatitis C virus (HCV) influences HIV reservoir size. However, it is unknown whether this coinfection also induces a higher provirus transcription. Viral transcription is promoted by synergy between cellular factors such as NF-κB and the viral regulator Tat. The impact of HCV coinfection on HIV provirus transcription was analyzed in resting (r)CD4 T+ cells (CD3+CD4+CD25-CD69-HLADR-) and rCD4 T cells-depleted PBMCs (rCD4 T- PBMCs) from a multicenter cross-sectional study of 115 cART-treated HIV patients: 42 HIV+/HCV+ coinfected individuals (HIV+/HCV+), 34 HIV+ patients with HCV spontaneous clearance (HIV+/HCV−) and 39 HIV patients (HIV+). Viral transcription was assessed in total RNA through the quantification of unspliced, single spliced, and multiple spliced viral mRNAs by qPCR. Linear correlations between viral reservoir size and viral splicing were determined. A 3-fold increase of multiple spliced transcripts in rCD4 T+ cells of HIV+/HCV+ patients was found compared to HIV+ individuals (p < 0.05). As Tat is synthesized by multiple splicing, the levels of Tat were also quantified in these patients. Significant differences in single and multiple spliced transcripts were also observed in rCD4 T- PBMCs. Levels of multiple spliced mRNAs were increased in rCD4 T+ cells isolated from HIV+/HCV+ subjects, which could indicate a higher Tat activity in these cells despite their resting state. View Full-Text
Keywords: viral splicing; HIV/HCV; coinfection; HIV reservoir; resting CD4+ T lymphocytes; qPCR; Tat viral splicing; HIV/HCV; coinfection; HIV reservoir; resting CD4+ T lymphocytes; qPCR; Tat
Show Figures

Figure 1

MDPI and ACS Style

Martínez-Román, P.; López-Huertas, M.R.; Crespo-Bermejo, C.; Arca-Lafuente, S.; Cortegano, I.; Valle-Millares, D.; Gaspar, M.L.; Martín-Carbonero, L.; Domínguez-Domínguez, L.; Ryan, P.; de los Santos, I.; de la Fuente-Moral, S.; Fernández-Rodríguez, A.; Coiras, M.; Briz, V., on behalf of COVIHEP. Hepatitis C Virus Influences HIV-1 Viral Splicing in Coinfected Patients. J. Clin. Med. 2020, 9, 2091. https://doi.org/10.3390/jcm9072091

AMA Style

Martínez-Román P, López-Huertas MR, Crespo-Bermejo C, Arca-Lafuente S, Cortegano I, Valle-Millares D, Gaspar ML, Martín-Carbonero L, Domínguez-Domínguez L, Ryan P, de los Santos I, de la Fuente-Moral S, Fernández-Rodríguez A, Coiras M, Briz V on behalf of COVIHEP. Hepatitis C Virus Influences HIV-1 Viral Splicing in Coinfected Patients. Journal of Clinical Medicine. 2020; 9(7):2091. https://doi.org/10.3390/jcm9072091

Chicago/Turabian Style

Martínez-Román, Paula; López-Huertas, María R.; Crespo-Bermejo, Celia; Arca-Lafuente, Sonia; Cortegano, Isabel; Valle-Millares, Daniel; Gaspar, María L.; Martín-Carbonero, Luz; Domínguez-Domínguez, Lourdes; Ryan, Pablo; de los Santos, Ignacio; de la Fuente-Moral, Sara; Fernández-Rodríguez, Amanda; Coiras, Mayte; Briz, Verónica on behalf of COVIHEP. 2020. "Hepatitis C Virus Influences HIV-1 Viral Splicing in Coinfected Patients" J. Clin. Med. 9, no. 7: 2091. https://doi.org/10.3390/jcm9072091

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop