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Open AccessArticle

Multifunctional Properties of Quercitrin-Coated Porous Ti-6Al-4V Implants for Orthopaedic Applications Assessed In Vitro

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Group of Cell Therapy and Tissue Engineering, Department of Fundamental Biology and Health Sciences, Research Institute on Health Sciences (IUNICS), University of the Balearic Islands, 07122 Palma, Spain
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Health Research Institute of the Balearic Islands (IdISBa), 07010 Palma, Spain
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NuMat Medtech, S.L., 07121 Palma, Spain
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Orchid Orthopedics, 5404 Baden-Dätwill, Switzerland
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Orchid Orthopedic, Memphis, TN 38118, USA
*
Authors to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(3), 855; https://doi.org/10.3390/jcm9030855
Received: 21 February 2020 / Revised: 13 March 2020 / Accepted: 18 March 2020 / Published: 20 March 2020
(This article belongs to the Special Issue Latest Clinical And Basic Science Advances In Bone Regeneration)
(1) One strategy to improve the outcome of orthopedic implants is to use porous implants with the addition of a coating with an antibacterial biomolecule. In this study, we aimed to produce and test the biocompatibility, the osteopromotive (both under normal conditions and under a bacterial challenge with lipopolysaccharide (LPS)) and antibacterial activities of a porous Ti-6Al-4V implant coated with the flavonoid quercitrin in vitro. (2) Porous Ti-6Al-4V implants were produced by 3D printing and further functionalized with quercitrin by wet chemistry. Implants were characterized in terms of porosity and mechanical testing, and the coating with quercitrin by fluorescence staining. Implant biocompatibility and bioactivity was tested using MC3T3-E1 preosteoblasts by analyzing cytotoxicity, cell adhesion, osteocalcin production, and alkaline phosphatase (ALP) activity under control and under bacterial challenging conditions using lipopolysaccharide (LPS). Finally, the antibacterial properties of the implants were studied using Staphylococcus epidermidis by measuring bacterial viability and adhesion. (3) Porous implants showed pore size of about 500 µm and a porosity of 52%. The coating was homogeneous over all the 3D surface and did not alter the mechanical properties of the Young modulus. Quercitrin-coated implants showed higher biocompatibility, cell adhesion, and osteocalcin production compared with control implants. Moreover, higher ALP activity was observed for the quercitrin group under both normal and bacterial challenging conditions. Finally, S. epidermidis live/dead ratio and adhesion after 4 h of incubation was lower on quercitrin implants compared with the control. (4) Quercitrin-functionalized porous Ti-6Al-4V implants present a great potential as an orthopedic porous implant that decreases bacterial adhesion and viability while promoting bone cell growth and differentiation. View Full-Text
Keywords: Porous Ti-6Al-4V implants; quercitrin; multifunctional coating; ALP activity; osteocalcin release; additive manufacturing; orthopedic implants Porous Ti-6Al-4V implants; quercitrin; multifunctional coating; ALP activity; osteocalcin release; additive manufacturing; orthopedic implants
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MDPI and ACS Style

Llopis-Grimalt, M.A.; Arbós, A.; Gil-Mir, M.; Mosur, A.; Kulkarni, P.; Salito, A.; Ramis, J.M.; Monjo, M. Multifunctional Properties of Quercitrin-Coated Porous Ti-6Al-4V Implants for Orthopaedic Applications Assessed In Vitro. J. Clin. Med. 2020, 9, 855.

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