Subacute Thyroiditis is Associated with HLA-B*18:01, -DRB1*01 and -C*04:01—The Significance of the New Molecular Background

Stasiak, Magdalena; Tymoniuk, Bogusław; Michalak, Renata; Stasiak, Bartłomiej; Kowalski, Marek L.; Lewiński, Andrzej

doi:10.3390/jcm9020534

Open AccessArticle

Subacute Thyroiditis is Associated with HLA-B18:01, -DRB101 and -C*04:01—The Significance of the New Molecular Background

by

Magdalena Stasiak

¹

,

Bogusław Tymoniuk

²,

Renata Michalak

¹

,

Bartłomiej Stasiak

³,

Marek L. Kowalski

²

and

Andrzej Lewiński

^1,4,*

¹

Department of Endocrinology and Metabolic Diseases, Polish Mother’s Memorial Hospital Research Institute, 281/289 Rzgowska St., 93-338 Lodz, Poland

²

Department of Immunology, Rheumatology and Allergy, Medical University of Lodz, 251 Pomorska St, 92-213 Lodz, Poland

³

Institute of Information Technology, Lodz University of Technology, 215 Wolczanska St., 90-924 Lodz, Poland

⁴

Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, 281/289 Rzgowska St., 93-338 Lodz, Poland

^*

Author to whom correspondence should be addressed.

J. Clin. Med. 2020, 9(2), 534; https://doi.org/10.3390/jcm9020534

Submission received: 5 February 2020 / Accepted: 13 February 2020 / Published: 16 February 2020

(This article belongs to the Section Clinical Laboratory Medicine)

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Abstract

Subacute thyroiditis (SAT) is a thyroid inflammatory disease whose pathogenesis is still not completely defined. Previous viral infection is considered to be a triggering factor in genetically predisposed individuals. In about 70% of patients, susceptibility to SAT is associated with the HLA-B*35 allele. The correlation between SAT and other human leukocyte antigens (HLA) has not yet been unequivocally demonstrated and the genetic background is still unknown in about 30% of patients. The purpose of our study was to perform HLA genotyping using a next-generation sequencing method, to find out whether alleles other than HLA-B*35 are correlated with SAT morbidity. HLA-A, -B, -C, -DQB1, -DRB1 were genotyped using a next-generation sequencing method in 1083 subjects, including 60 SAT patients and 1023 healthy controls. Among 60 patients diagnosed with SAT, 81.7% of subjects were identified as having allele HLA-B*35, 23.3% had HLA-B*18:01, 28.3% had HLA-DRB1*01 and 75.5% had HLA-C*04:01. These alleles occurred in the control group at frequencies of 10.2%, 7.2%, 12.9% and 12.5%, respectively. The differences were statistically significant, with p < 0.05. In addition to its previously described relationship with HLA-B*35, genetic susceptibility to SAT was associated with the presence of HLA-B*18:01, DRB1*01 and C*04:01. The alleles HLA-B*18:01 and DRB1*01 were independent SAT risk factors. The assessment of these four alleles allows the confirmation of genetic predisposition in almost all patients with SAT.

Keywords: HLA-B*35; HLA-B*18:01; HLA-DRB1*01; HLA-C*04:01; subacute thyroiditis

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MDPI and ACS Style

Stasiak, M.; Tymoniuk, B.; Michalak, R.; Stasiak, B.; Kowalski, M.L.; Lewiński, A. Subacute Thyroiditis is Associated with HLA-B*18:01, -DRB1*01 and -C*04:01—The Significance of the New Molecular Background. J. Clin. Med. 2020, 9, 534. https://doi.org/10.3390/jcm9020534

AMA Style

Stasiak M, Tymoniuk B, Michalak R, Stasiak B, Kowalski ML, Lewiński A. Subacute Thyroiditis is Associated with HLA-B*18:01, -DRB1*01 and -C*04:01—The Significance of the New Molecular Background. Journal of Clinical Medicine. 2020; 9(2):534. https://doi.org/10.3390/jcm9020534

Chicago/Turabian Style

Stasiak, Magdalena, Bogusław Tymoniuk, Renata Michalak, Bartłomiej Stasiak, Marek L. Kowalski, and Andrzej Lewiński. 2020. "Subacute Thyroiditis is Associated with HLA-B*18:01, -DRB1*01 and -C*04:01—The Significance of the New Molecular Background" Journal of Clinical Medicine 9, no. 2: 534. https://doi.org/10.3390/jcm9020534

APA Style

Stasiak, M., Tymoniuk, B., Michalak, R., Stasiak, B., Kowalski, M. L., & Lewiński, A. (2020). Subacute Thyroiditis is Associated with HLA-B*18:01, -DRB1*01 and -C*04:01—The Significance of the New Molecular Background. Journal of Clinical Medicine, 9(2), 534. https://doi.org/10.3390/jcm9020534

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Subacute Thyroiditis is Associated with HLA-B18:01, -DRB101 and -C*04:01—The Significance of the New Molecular Background

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