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Article

Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography

1
Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Korea
2
Department of Surgery, Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul 07061, Korea
*
Authors to whom correspondence should be addressed.
These authors are the first co-author.
J. Clin. Med. 2020, 9(2), 301; https://doi.org/10.3390/jcm9020301
Received: 11 December 2019 / Revised: 16 January 2020 / Accepted: 18 January 2020 / Published: 21 January 2020
(This article belongs to the Special Issue Circulating Biomarkers as a Liquid Biopsy for Cancer)
Technologies for the detection and isolation of circulating tumor cells (CTCs) are essential in liquid biopsy, a minimally invasive technique for early diagnosis and medical intervention in cancer patients. A promising method for CTC capture, using an affinity-based approach, is the use of functionalized hydrogel microparticles (MP), which have the advantages of water-like reactivity, biologically compatible materials, and synergy with various analysis platforms. In this paper, we demonstrate the feasibility of CTC capture by hydrogel particles synthesized using a novel method called degassed mold lithography (DML). This technique increases the porosity and functionality of the MPs for effective conjugation with antibodies. Qualitative fluorescence analysis demonstrates that DML produces superior uniformity, integrity, and functionality of the MPs, as compared to conventional stop flow lithography (SFL). Analysis of the fluorescence intensity from porosity-controlled MPs by each reaction step of antibody conjugation elucidates that more antibodies are loaded when the particles are more porous. The feasibility of selective cell capture is demonstrated using breast cancer cell lines. In conclusion, using DML for the synthesis of porous MPs offers a powerful method for improving the cell affinity of the antibody-conjugated MPs. View Full-Text
Keywords: circulating tumor cell; cell capture; hydrogel microparticle; degassed mold lithography circulating tumor cell; cell capture; hydrogel microparticle; degassed mold lithography
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MDPI and ACS Style

Lee, N.J.; Maeng, S.; Kim, H.U.; Roh, Y.H.; Hwang, C.; Kim, J.; Hwang, K.-T.; Bong, K.W. Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography. J. Clin. Med. 2020, 9, 301. https://doi.org/10.3390/jcm9020301

AMA Style

Lee NJ, Maeng S, Kim HU, Roh YH, Hwang C, Kim J, Hwang K-T, Bong KW. Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography. Journal of Clinical Medicine. 2020; 9(2):301. https://doi.org/10.3390/jcm9020301

Chicago/Turabian Style

Lee, Nak J., Sejung Maeng, Hyeon U. Kim, Yoon H. Roh, Changhyun Hwang, Jongjin Kim, Ki-Tae Hwang, and Ki W. Bong 2020. "Affinity-Enhanced CTC-Capturing Hydrogel Microparticles Fabricated by Degassed Mold Lithography" Journal of Clinical Medicine 9, no. 2: 301. https://doi.org/10.3390/jcm9020301

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