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Article

Molecular Detection of Mycobacterium tuberculosis in Oral Mucosa from Patients with Presumptive Tuberculosis

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Institut d’Investigació Germans Trias i Pujol, CIBER Enfermedades Respiratorias (CIBERES), Carretera del Canyet s/n, Camí de les Escoles s/n, Badalona, 08916 Barcelona, Spain
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Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Carretera del Canyet s/n Badalona, 08916 Barcelona, Spain
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Institutia Medico-Sanitara Publica, Institutul de Ftiziopneumologie “Chiril Draganiuc”, Strada Constantin Vârnav 13, 2025 Chișinău, Moldova
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Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht University, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
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Centre for Drugs and Diagnostics, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
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School of Medicine, University of St Andrews, North Haugh, St Andrews KY16 9TF, UK
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Department of Clinical Sciences, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
*
Author to whom correspondence should be addressed.
These authors have contributed equally to this work.
Senior co-authors.
J. Clin. Med. 2020, 9(12), 4124; https://doi.org/10.3390/jcm9124124
Received: 10 November 2020 / Revised: 4 December 2020 / Accepted: 16 December 2020 / Published: 21 December 2020
(This article belongs to the Special Issue Recent Advances in Mycobacterial Research)
Tuberculosis (TB) diagnosis is increasingly based on the detection of Mycobacterium tuberculosis complex (MTBC) DNA in sputum using molecular diagnostic tests as the first test for diagnosis. However, sputum can be difficult to obtain in children, patients without productive cough, and the elderly and approaches testing non-sputum samples are needed. We evaluated whether TB can be detected from the oral mucosa of patients with TB. Adults with presumptive TB were examined using culture, Xpert MTB/RIF, smear microscopy and X-Rays. Oral mucosa swabs collected on PrimeStore-MTM, stored at room temperature if tested within 30 days or at −20 °C if examined at a later time. RT-PCR was performed to detect M. tuberculosis DNA. Eighty patients had bacteriologically-confirmed TB, 34 had bacteriologically-negative TB (negative tests but abnormal X-rays) and 152 were considered not to have TB (not TB). Oral swabs RT-PCR were positive in 29/80 (36.3%) bacteriologically-confirmed, 9/34 (26.5%) bacteriologically-negative and 29/152 (19.1%) not TB. The yield varied among samples stored for less and more than 30 days (p = 0.013) from 61% (11/18) and 29% (18/62) among bacteriologically confirmed, and 30.8% (4/13) and 23.8% (5/21) among bacteriologically-negative participants. Among not TB patients, the specificity was 80.9% (123/152), being 78.3% (18/23) among samples stored less than 30 days and 81.4% (105/129) among samples stored for more than 30 days (p = 0.46). The detection of M. tuberculosis in oral mucosa samples is feasible, but storage conditions may affect the yield. View Full-Text
Keywords: oral swab; diagnosis; real-time PCR oral swab; diagnosis; real-time PCR
MDPI and ACS Style

Molina-Moya, B.; Ciobanu, N.; Hernandez, M.; Prat-Aymerich, C.; Crudu, V.; Adams, E.R.; Codreanu, A.; Sloan, D.J.; Cuevas, L.E.; Dominguez, J. Molecular Detection of Mycobacterium tuberculosis in Oral Mucosa from Patients with Presumptive Tuberculosis. J. Clin. Med. 2020, 9, 4124. https://doi.org/10.3390/jcm9124124

AMA Style

Molina-Moya B, Ciobanu N, Hernandez M, Prat-Aymerich C, Crudu V, Adams ER, Codreanu A, Sloan DJ, Cuevas LE, Dominguez J. Molecular Detection of Mycobacterium tuberculosis in Oral Mucosa from Patients with Presumptive Tuberculosis. Journal of Clinical Medicine. 2020; 9(12):4124. https://doi.org/10.3390/jcm9124124

Chicago/Turabian Style

Molina-Moya, Barbara, Nelly Ciobanu, Marta Hernandez, Cristina Prat-Aymerich, Valeriu Crudu, Emily R. Adams, Alexandru Codreanu, Derek J. Sloan, Luis E. Cuevas, and Jose Dominguez. 2020. "Molecular Detection of Mycobacterium tuberculosis in Oral Mucosa from Patients with Presumptive Tuberculosis" Journal of Clinical Medicine 9, no. 12: 4124. https://doi.org/10.3390/jcm9124124

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