Differential Protein Expression in Human Dental Pulp: Comparison of Healthy, Inflamed, and Traumatic Pulp
Abstract
:1. Introduction
2. Experimental Section
2.1. Sample Collection and Preparation
- Healthy pulp (n = 1; 22/M): two healthy and completely erupted permanent human third molars with closed apex, which were caries free showing normal probing depths.
- Inflamed pulp group (n = 2; 23/F and 35/M): maxillary central incisors with advanced dental caries, defined as the presence of spontaneous pain and lingering response to the cold test.
- Traumatic pulp group (n = 2; 24/F and 35/M): maxillary central incisors with closed apex, ten days after replantation due to traumatic avulsion and avulsed time within 20 min.
2.2. Two-Dimensional Electrophoresis
2.3. Spot Selection for Identification
- Spots in the inflamed and traumatic pulp group with an intensity greater than 2.0 times that in the healthy pulp group
- Spots in the inflamed or traumatic pulp group with an intensity greater than 10.0 times that in the healthy pulp group
2.4. In-Gel Tryptic Digestion
2.5. LC-MS/MS for Peptide Analysis
2.6. Database Search
3. Results
3.1. 2-DE and Image Analysis
3.2. Spot Selection for Protein Identification
3.3. LC-MS/MS for Peptide Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Spot ID | Protein Name | Protein Score | Peptide Matched | Coverage (%) | Healthy Pulp (Vol.%) | Inflamed Pulp1 (Vol.%) | Inflamed Pulp2 (Vol.%) | Traumatic Pulp1 (Vol.%) | Traumatic Pulp2 (Vol.%) |
---|---|---|---|---|---|---|---|---|---|
742 | keratin, type II | 325 | 11 | 12 | 0.080 | 0.162 | 0.799 | 0.518 | 0.617 |
757 | keratin, type II | 449 | 13 | 12 | 0.480 | 1.141 | 4.742 | 2.024 | 3.532 |
772 | annexin V chain A | 194 | 7 | 13 | 0.156 | 0.412 | 1.450 | 0.657 | 0.632 |
1186 | hemoglobin chain B | 649 | 32 | 61 | 0.905 | 5.430 | 5.675 | 5.584 | 16.633 |
1189 | keratin, type II | 526 | 13 | 16 | 0.839 | 9.564 | 10.279 | 4.387 | 22.620 |
1195 | hemoglobin alpha 1-2 hybrid | 449 | 22 | 66 | 0.110 | 0.771 | 1.249 | 3.508 | 8.119 |
554 | gelsolin isoform a precursor | 152 | 4 | 3 | 0.028 | * 0.328 | * 0.300 | ||
793 | annexin V chain A | 739 | 20 | 39 | 0.030 | 0.401 | * 1.274 | ||
853 | mutant beta-actin | 193 | 7 | 13 | 0.043 | * 0.448 | 0.373 | ||
1062 | unnamed protein product | 744 | 25 | 20 | 0.059 | * 1.725 | * 0.897 | ||
1083 | unnamed protein product | 866 | 27 | 26 | 0.025 | * 1.124 | 0.174 | ||
1183 | unnamed protein product | 441 | 15 | 16 | 0.096 | * 1.471 | 0.301 | ||
1188 | hemoglobin chain B | 469 | 24 | 75 | 0.306 | 1.228 | 1.303 | 1.457 | * 4.523 |
1194 | hemoglobin chain A | 257 | 10 | 31 | 0.088 | * 0.808 | * 1.636 | ||
1217 | hemoglobin chain A | 259 | 11 | 42 | 0.102 | 0.402 | * 1.049 |
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Yue, W.; Kim, S.; Jung, H.-S.; Lee, J.-M.; Lee, S.; Kim, E. Differential Protein Expression in Human Dental Pulp: Comparison of Healthy, Inflamed, and Traumatic Pulp. J. Clin. Med. 2019, 8, 1234. https://doi.org/10.3390/jcm8081234
Yue W, Kim S, Jung H-S, Lee J-M, Lee S, Kim E. Differential Protein Expression in Human Dental Pulp: Comparison of Healthy, Inflamed, and Traumatic Pulp. Journal of Clinical Medicine. 2019; 8(8):1234. https://doi.org/10.3390/jcm8081234
Chicago/Turabian StyleYue, Wonyoung, Sunil Kim, Han-Sung Jung, Jong-Min Lee, Sukjoon Lee, and Euiseong Kim. 2019. "Differential Protein Expression in Human Dental Pulp: Comparison of Healthy, Inflamed, and Traumatic Pulp" Journal of Clinical Medicine 8, no. 8: 1234. https://doi.org/10.3390/jcm8081234