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Differential Protein Expression in Human Dental Pulp: Comparison of Healthy, Inflamed, and Traumatic Pulp

1
Microscope Center, Department of Conservative Dentistry and Oral Science Research Center, College of Dentistry, Yonsei University, Seoul 03722, Korea
2
Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Korea
3
Department of Applied Life Science, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Korea
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2019, 8(8), 1234; https://doi.org/10.3390/jcm8081234
Received: 20 July 2019 / Accepted: 14 August 2019 / Published: 16 August 2019
(This article belongs to the Special Issue Innovations in Endodontic Dentistry)
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Abstract

Trauma or injury to the dental pulp causes inflammation. This study compared the proteome of healthy pulp with inflamed pulp and traumatic pulp to identify the differentially expressed proteins in the diseased state. Five participants were grouped based on the pulpal status of the teeth: healthy, inflamed, or traumatic pulp. Pulp was extirpated and stored immediately in liquid nitrogen. Pulp tissues were subjected to 2-dimensional gel electrophoresis, and spot selection was performed. The selected spots were analyzed using liquid chromatography-tandem mass spectrometry and identified by correlating mass spectra to the proteomic databases. Fifteen spots showed increased expression in the inflamed and traumatic pulp. Annexin V, type II keratin, and hemoglobin levels were increased two-fold in the inflamed and traumatic pulp group and annexin V, mutant beta-actin, and hemoglobin were increased by ten-fold in the inflamed or traumatic pulp group, compared to levels in the healthy pulp group. Annexin V constituted two out of fifteen protein spots, and seemed to play a critical role in inhibiting inflammation and promoting the immune reaction. Further studies on this protein concerning its role in pulp repair are necessary to elucidate the underlying mechanisms. View Full-Text
Keywords: dental pulp; dental pulp inflammation; proteome; dentin-pulp complex regeneration; annexin V; mass spectrometry dental pulp; dental pulp inflammation; proteome; dentin-pulp complex regeneration; annexin V; mass spectrometry
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Yue, W.; Kim, S.; Jung, H.-S.; Lee, J.-M.; Lee, S.; Kim, E. Differential Protein Expression in Human Dental Pulp: Comparison of Healthy, Inflamed, and Traumatic Pulp. J. Clin. Med. 2019, 8, 1234.

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