Twin-Twin Transfusion Syndrome with and without Selective Fetal Growth Restriction Prior to Fetoscopic Laser Surgery: Short and Long-Term Outcome
by
,
Sophie G. Groene
1,*
,
Lisanne S. A. Tollenaar
2,
Jeanine M. M. van Klink
1,
Monique C. Haak
2,
Frans J. C. M. Klumper
2,
Johanna M. Middeldorp
2,
Dick Oepkes
2,
Femke Slaghekke
2 and
Enrico Lopriore
1 1
Division of Neonatology, Department of Pediatrics, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands
2
Division of Fetal Medicine, Department of Obstetrics, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2019, 8(7), 969; https://doi.org/10.3390/jcm8070969
Submission received: 13 June 2019
/
Revised: 2 July 2019
/
Accepted: 2 July 2019
/
Published: 3 July 2019
(This article belongs to the Special Issue Diagnosis, Treatment and Outcome in Complicated Monochorionic Twins)
Abstract
:As twin-twin transfusion syndrome (TTTS) and selective fetal growth restriction (sFGR) are both prevalent complications of monochorionic (MC) twin pregnancies, its coexistence is not uncommon. The aim of this study is to evaluate the short and long-term outcome in TTTS with and without sFGR prior to fetoscopic laser coagulation. All TTTS cases treated with laser surgery at our center between 2001–2019 were retrospectively reviewed for the presence of sFGR, defined as an estimated fetal weight (EFW) <10th centile. We compared two groups: TTTS-only and TTTS + sFGR. Primary outcomes were perinatal survival and long-term severe neurodevelopmental impairment (NDI). Of the 527 pregnancies eligible for analysis, 40.8% (n = 215) were categorized as TTTS-only and 59.2% (n = 312) as TTTS + sFGR. Quintero stage at presentation was higher in the TTTS + sFGR group compared to the TTTS-only group (57% compared to 44% stage III). Separate analysis of donors showed significantly lower perinatal survival for donors in the TTTS + sFGR group (72% (224/311) compared to 81% (173/215), p = 0.027). Severe NDI at follow-up in long-term survivors in the TTTS-only and TTTS + sFGR group was present in 7% (13/198) and 9% (27/299), respectively (p = 0.385). Both sFGR (OR 1.5;95% CI 1.1–2.0, p = 0.013) and lower gestational age at laser (OR 1.1;95% CI 1.0–1.1, p = 0.001) were independently associated with decreased perinatal survival. Thus, sFGR prior to laser surgery is associated with a more severe initial presentation and decreased donor perinatal survival. The long-term outcome was not affected.
1. Introduction
Twin-twin transfusion syndrome (TTTS) is a disorder arising from inter-twin blood flow imbalances caused by vascular anastomoses on the surface of the shared placenta in monochorionic (MC) twins [1]. The donor transfers blood to the recipient, resulting in hypovolemia in the donor and hypervolemia in the recipient. Due to the decreased blood volume, the donor may present with growth restriction, next to the oliguria and oligohydramnios, while the recipient suffers from polyhydramnios [2]. If left untreated, TTTS has a high perinatal mortality rate [3]. Additionally, long-term outcomes show an increased risk of neurodevelopmental impairment (NDI) [4]. At present, fetoscopic laser surgery is the preferred treatment for TTTS, coagulating the placental vascular equator after selective coagulation of the anastomoses, called the Solomon technique [5,6].
Selective fetal growth restriction (sFGR) occurs in 10%–15% of MC pregnancies and is primarily due to unequal placental sharing [7]. sFGR is defined as an estimated fetal weight (EFW) <10th centile for the gestational age and/or a birth-weight discordance >25% [8]. Complications of sFGR include fetal deterioration, fetal demise, prematurity and neonatal morbidity or mortality [9]. NDI rates at follow-up appear to be high as well, with a disadvantage for the smaller twin [10,11,12].
As TTTS and sFGR are both prevalent complications of MC twin pregnancies, its coexistence is not uncommon. Approximately two-thirds of pregnancies complicated by TTTS present with coexistent sFGR, suggesting an underlying association between the pathogeneses of both disorders [13]. To understand this association better, placental analysis is necessary, with the quantification of the individual fetal territories. Moreover, coexistent sFGR prior to fetoscopic laser surgery might lead to a distinct course of the disease. More insight in the outcomes of TTTS with coexistent sFGR can thus result in a more specific prognostication, identifying risks and corresponding management options in an early stage. The aim of this study is to evaluate the impact of coexistent sFGR on short and long-term outcomes in MC twin pregnancies complicated by TTTS treated with fetoscopic laser coagulation.
2. Materials and Methods
In this retrospective study all consecutive TTTS cases treated with fetoscopic laser coagulation at our center between 2001 and 2019 were eligible for inclusion. Our center is the national referral center for fetal therapy in The Netherlands. From 2012 onwards, the Solomon technique was universally performed in our center, after conducting the Solomon trial from 2007 onwards. TTTS was diagnosed using the Eurofoetus criteria [14] and staged using the standard system [15]. All TTTS cases were reviewed for the presence of sFGR, defined as an EFW <10th centile prior to laser surgery. If so, the case was classified as ‘TTTS + sFGR’. If not, it was categorized as ‘TTTS-only’. The EFW before the laser had to be documented for both twins. We excluded MC triplet pregnancies, cases with concurrent twin anemia-polycythemia sequence (TAPS) [16], cases with incomplete laser surgery (either recurrent TTTS or post-laser TAPS) and cases in which a selective reduction or termination of pregnancy (TOP) was performed after a laser attempt.
The following maternal and neonatal baseline characteristics were collected from the medical records: Maternal age, gravidity, parity, gender, presence of umbilical artery (UA) Doppler abnormalities (defined as persistent or intermittent absent or reversed end-diastolic flow (A/REDF) prior to laser), Quintero staging, gestational age at birth, mode of delivery, birth weight, birth weight discordance (calculated as (birth weight large twin − birth weight smaller twin)/birth weight larger twin × 100%), proportion of pregnancies with a birth weight discordance >25%, proportion of neonates born small for gestational age (SGA) (defined as a birth weight <10th centile) and proportion of donor twins being the smaller twin at birth.
The number and type of placental anastomoses (arterio-venous (AV), veno-arterial (VA), arterio-arterial (AA) and veno-venous (VV)) was determined during the fetoscopic laser coagulation. After birth, routine placental injection was performed using colored dye to visualize vascular patterns at the placental surface [17]. The injected placenta was evaluated for residual anastomoses along the laser demarcation line, the placenta margin and in the membranes. Umbilical cord insertions were categorized into central, velamentous or marginal [18]. Fetal territories were differentiated by the margins of the twin-specific dyes and/or the laser demarcation line and expressed as a percentage of the total placental area. Placental share discordance was calculated as follows: (Placental share large twin − placental share small twin)/placental share large twin × 100%.
Neurodevelopmental assessment was performed at two years of age using the Bayley Scales of Infant and Toddler Development second and third edition (Bayley-II and III) according to the routine care following fetal therapy in our center. Severe NDI was defined as cerebral palsy (CP) ≥grade II according to the Gross Motor Function Classification System (GMFCS) [19], impaired cognitive and/or motor development (IQ score <70 (−2SD)) or bilateral blindness and/or deafness (requiring amplification).
Primary outcomes were perinatal survival and long-term severe NDI. Secondary outcomes included fetal death (within 24 h of laser), neonatal mortality defined as death within 28 days after birth, disease-free survival defined as survival without severe NDI, and severe neonatal morbidity consisting of at least one of the following conditions: Respiratory distress syndrome (RDS) requiring mechanical ventilation or surfactant, patent ductus arteriosus requiring medical treatment or surgical closure, necrotizing enterocolitis ≥stage 2 [20] and severe cerebral injury (defined as cystic periventricular leukomalacia (PVL) ≥grade 2 [21], intraventricular hemorrhage (IVH) ≥grade 3 [22], ventricular dilatation >97th percentile [23], arterial or venous infarct, or porencephalic or parenchymal cysts). Outcomes were compared between TTTS-only and TTTS + sFGR.
Statistical data were analyzed using the IBM statistics v23.0 (SPSS, Inc., an IBM company, Chicago, IL, USA). Data are presented as median with interquartile ranges, n/N (%) or n (%). A Mann-Whitney U test and a Chi-square test were used to analyze numerical data and categorical data, respectively. Data on survival and morbidity were analyzed using a Generalized Estimated Equation (GEE) when appropriate. A univariate linear regression model for identification of potential predictors of perinatal survival was performed. When antenatal baseline or placental characteristics differed between TTTS-only and TTTS + sFGR groups (p < 0.05), these were subsequently included in the univariate analysis along with characteristics previously described in literature. A separate analysis was performed for the TTTS + sFGR group to identify which cases are most at risk of perinatal death. When a significant association was found in the univariate analysis, the variable was included in the multivariate linear regression model. A p-value <0.05 was considered statistically significant for all analyses.
3. Results
Of the 696 twin pregnancies complicated by TTTS and treated with laser therapy, 54 had missing or incomplete records of EFW before laser coagulation (Figure 1). After exclusion according to the aforementioned criteria, 527 pregnancies were eligible for analysis, of which 40.8% (n = 215) were categorized as TTTS-only and 59.2% (n = 312) as TTTS + sFGR.
Eleven selective reductions/TOPs were performed for varied reasons, among which recurrent TTTS (n = 7), incomplete laser with severe growth restriction after laser (n = 1), fetal abnormalities (n = 1) and donor fetal demise with combined cerebral injury in the recipient (n = 2). All these cases were excluded from the analyses.
Baseline maternal and neonatal characteristics are summarized in Table 1. The TTTS + sFGR group had a significantly higher portion of females (53% (330/618) compared to 46% (196/424), p = 0.023). Of the 123 fetuses that presented with UA Doppler abnormalities prior to laser, 88% (108/123) were donors. In five cases, both donor and recipient simultaneously presented with similar UA Doppler abnormalities. Both persistent A/REDF (12% (73/592) compared to 4% (16/410)) and intermittent A/REDF (4% (24/592) compared to 2% (10/410)) were more frequently present in the TTTS + sFGR group (p < 0.0001). The distribution of Quintero stages differed significantly between groups. While most cases in the TTTS-only group had Quintero II stage at presentation (44%) the TTTS + sFGR groups presented predominantly with Quintero III stage (57%) (p < 0.0001). Gestational age at birth was 33.1 (29.3–35.9) weeks and 33.0 (29.7–35.7) weeks in the TTTS-only and TTTS + sFGR group, respectively (p = 0.780). In the TTTS + sFGR group, the birth weight of donor twins was lower and the birth weight discordance was higher compared to the TTTS-only group. The proportion of neonates born SGA was 21% (73/352) in the TTTS-only group and 49% (231/465) in the TTTS + sFGR group (p < 0.0001).
A comparison of laser surgery outcomes and placental characteristics is presented in Table 2. Significant differences in placental angioarchitecture were detected between the two groups. Placentas in the TTTS + sFGR group more often had an AA anastomosis, velamentous cord insertions and unequal placental sharing (Figure 2).
Short- and long-term outcomes are presented in Table 3. In our population, 673 infants were eligible for follow-up evaluation at two years, of which 162 (24.1%) were lost to follow-up, largely explained by lack of follow-up between 2006–2007 due to organizational issues (Figure 3). Baseline characteristics between infants with available follow-up and infants lost to follow-up did not differ, except for sex with 42% females lost to follow-up and 53% females with available follow-up (p = 0.010). Three neonates had no available information on neonatal mortality, and thus perinatal survival, as they were delivered elsewhere and we received no further information. Donor fetal death rate was 24% (76/312) in the TTTS + sFGR group and 17% (36/215) in the TTTS-only group (p = 0.036). Perinatal survival in the TTTS-only and TTTS + sFGR group was 79% (339/429) and 75% (464/622), respectively (p = 0.176). Separate analysis of donor and recipient showed a significantly lower perinatal survival for donors in the TTTS + sFGR group (72% (224/311) compared to 81% (173/215), p = 0.027). Severe NDI at follow-up in long-term survivors was present in 7% (13/198) and 9% (27/299) in the TTTS-only and TTTS + sFGR group, respectively (p = 0.385). Disease-free survival (including 248 deaths and 497 cases with complete follow-up) was found in 64% (185/288) of the TTTS-only group and 59% (269/457) of the TTTS + sFGR group (p = 0.159). Regarding other short-term outcomes, neonatal mortality for recipients was 6% (10/176) in the TTTS-only group as opposed to 1% (3/243) in the TTTS + sFGR group (p = 0.010).
Three factors appeared to be univariately associated with perinatal survival in the entire cohort: The presence of sFGR prior to laser surgery (OR 1.4;95% CI 1.1–1.8, p = 0.016), persistent A/REDF in the UA prior to laser (OR 1.9;95% CI 1.2–3.0, p = 0.006) and gestational age at laser (OR 1.1;95% CI 1.0–1.1, p = 0.003) (Table S1). After multivariate analysis, sFGR (OR 1.4;95% CI 1.1–1.9, p = 0.049) and gestational age at laser (OR 1.1;95% CI 1.0–1.1, p = 0.009) were independently associated with perinatal survival. The univariate linear regression analysis of potential predictors for perinatal survival in the TTTS + sFGR group was performed as well (Table 4). Persistent A/REDF in the UA prior to laser (OR 1.9;95% CI 1.1–3.1, p = 0.018) and gestational age at laser (OR 1.1;95% CI 1.0–1.2, p = 0.008) were univariately associated with perinatal survival. Both persistent A/REDF in the UA prior to laser (OR 1.7;95% CI 1.0–2.9, p = 0.049) and gestational age at laser (OR 1.1;95% CI 1.0–1.1, p = 0.023) were identified as independent risk factors for perinatal death.
4. Discussion
Our study shows that the donor perinatal survival is significantly decreased (72% compared to 81%) in TTTS cases with coexistent sFGR prior to laser surgery, mainly caused by an increased donor fetal death rate (24% compared to 17%). sFGR prior to laser surgery appeared to be an independent predictor of decreased perinatal survival. Within the TTTS + sFGR group, those with persistent A/REDF in the UA prior to laser (mainly donors) were especially at risk. Yet, the rate of severe NDI in long-term survivors at follow-up was not significantly different for TTTS-only and TTTS + sFGR cases. These findings imply that coexistent sFGR prior to laser surgery negatively influences perinatal survival, but does not affect short-term morbidities or long-term outcome. Moreover, we found that almost 60% of TTTS cases presented with coexistent sFGR, a percentage similar to what has been described by previous research [13]. As expected, TTTS pregnancies complicated by sFGR had a higher Quintero stage at presentation, as the Quintero III stage is per definition characterized by UA Doppler abnormalities.
In this study we did not only focus on the short and long-term outcomes, but also evaluated the placental differences between the three groups, since placental abnormalities are one of the most important substrates and causes of sFGR. As expected, individual placental shares were more unequally distributed in TTTS + sFGR cases. In line with typical placental characteristics of sFGR [24], both AA anastomoses and velamentous cord insertions were more frequently present in the TTTS + sFGR placenta’s. In isolated sFGR cases, AA anastomoses can allow for compensatory flow from the large twin to the smaller twin, resulting in partial reliance of the small twin on its co-twin for nutrients. Laser surgery in TTTS + sFGR cases results in occlusion of these anastomoses, possibly leading to further growth restriction or even fetal demise of the small twin (primarily the donor) as its already meager nutrient supply is cut even further. Moreover, laser surgery causes damage to the placenta, even though effort is made to minimalize this as much as possible. When a small twin loses even a small part of its already limited placental share, this can have detrimental consequences for its further growth and survival. These factors suggest that the increased risk of donor fetal death exists mainly due to iatrogenic damage to the placenta that twins with a small placental share cannot compensate for. Nevertheless, laser surgery is still the leading therapy for TTTS as it is live-saving for both twins, whether it might be more disadvantageous to the growth restricted twin or not. Our placental findings as well as the common coexistence of TTTS and sFGR illustrate the intricately related pathophysiology of both disorders originating from the unequal distribution and the angioarchitecture of the placenta.
Furthermore, the total proportion of neonates born SGA is 49% in the TTTS + sFGR group, implying that a proportion of growth restricted twins has experienced catch-up growth after laser. The prevalence of catch-up growth after laser surgery of growth restricted donor fetuses in TTTS cases has been described before [25]. However, this catch-up growth does not always occur. A distinction between two possible mechanisms of coexistent sFGR can be made. One results from the unequal placental sharing as described earlier, while the other results from the imbalanced flow where the donor relinquishes its nutrients to its co-twin. One can imagine that a donor twin with a relatively equally shared placenta but growth-restricted due to flow imbalances will benefit the most from the laser surgery. When the donor twin already has a small placental share, laser surgery might result in a bigger loss of placental share leading to further growth restriction. These two mechanisms possibly coexist together. Understanding them might lead to a better prediction of when catch-up growth after laser will or will not occur.
Our results concerning survival of TTTS cases with coexistent sFGR are similar to what has previously been reported [13]. Van Winden et al. found a markedly decreased 30-day survival rate of the donor twin in the TTTS + sFGR group (84% compared to 75%), very similar to our results. Patients in the TTTS + sFGR group were twice as likely to experience a donor fetal demise as compared to the TTTS-only group (20% compared to 10%). Although we observed a similar outcome, the effect of sFGR on donor fetal death rate was not as large as described by Van Winden et al. Lastly, they found that TTTS cases with donor twin growth restriction and UA Doppler abnormalities (Quintero III donor-involved) had the highest risk of 30-day non-survival. Similarly, we identified persistent A/REDF in the UA prior to laser as an independent risk factor for perinatal death in our TTTS + sFGR population. Thus, our results are in line with previous research, confirming the negative influence of coexistent sFGR on survival in TTTS cases.
Another study conducted by our own research group [26] identified an EFW discordance (OR 1.0;95% CI 1.0–1.1, p = 0.04), UA Doppler abnormalities (OR 3.0;95% CI 1.1–8.0, p = 0.01) and the presence of AA anastomoses (OR 5.6;95% CI 2.2–14.0, p = 0.03) as independent risk factors for single donor fetal demise after laser. This study population consisted of a small portion (n = 273) of our total population in the current study. We found a significantly increased donor fetal death rate for TTTS + sFGR cases and sFGR in the whole population and persistent A/REDF in the UA for the TTTS + sFGR cases as independent risk factors for perinatal death, supporting their results. However, our multivariate regression analysis did not identify an EFW discordance >25% or AA anastomoses prior to laser as risk factors for perinatal death. As we did not use an EFW discordance in our initial definition of sFGR and we did not solely analyze single fetal demise but also included double fetal demise and neonatal mortality, discrepancies between our results are not unimaginable. Hence, our studies should be regarded as separate entities describing specific distinctive outcomes. Importantly, the number of cases included in the current study is much larger, therefore increasing the statistical power and decreasing the likelihood of a Type II error.
Several limitations should be taken into account when interpreting our results. Due to the retrospective nature of the study some data were missing, possibly introducing bias into our results. Prospective research focused on TTTS and coexistent sFGR prior to laser surgery is necessary to provide further evidence of superior quality. Nevertheless, our results are reinforced by 1) the relatively large study population, 2) the elaborate placental analysis allowing for a better understanding of the pathophysiology, 3) the extensive documentation of neonatal morbidities and 4) the standardized long-term follow-up giving insight into the lasting effects of TTTS and coexistent sFGR on neurodevelopmental outcome. Combining these aspects, we think our study provides reliable insight in the short and long-term outcomes of TTTS + sFGR pregnancies.
In conclusion, this study shows that TTTS + sFGR prior to fetoscopic laser surgery is present in nearly 60% of TTTS cases and results from typical placental abnormalities (more unequal sharing, AA anastomoses and velamentous cord insertion). TTTS with coexistent sFGR is associated with a more severe initial presentation and a decreased donor perinatal survival, mainly due to an increased donor fetal death rate. sFGR was independently associated with decreased perinatal survival. Those with coexistent sFGR and persistent A/REDF in the UA prior to laser are especially at risk. The rate of long-term severe NDI does not seem to differ from TTTS cases without sFGR. Larger prospective studies are needed to confirm (or refute) our findings and provide more conclusive evidence. Yet, our extensive overview of short and long-term outcomes can aid clinicians in proper prognostication, risk factor identification and parent counseling in the future.
Supplementary Materials
The following are available online at https://www.mdpi.com/2077-0383/8/7/969/s1, Table S1: Analysis of potential predictors of perinatal survival after laser surgery for TTTS.
Author Contributions
Conceptualization, S.G., L.T., J.K. and E.L; Methodology, S.G., L.T., J.K. and E.L.; Validation, S.G., L.T., J.K. and E.L.; Formal analysis, S.G., L.T., J.K. and E.L.; Investigation, S.G., L.T., J.K., M.H., F.K., J.M., D.O., F.S. and E.L.; Data curation, S.G., L.T., J.K. and E.L.; Writing—original draft preparation, S.G. and L.T.; Writing—review and editing, S.G., L.T., J.K., M.H., F.K., J.M., D.O., F.S. and E.L.; Visualization, S.G., L.T., J.K. and E.L.; Supervision, J.K. and E.L.; Project administration, S.G., L.T., J.K. and E.L.
Funding
This research was funded by the Dutch Heart Foundation, grant number 2017T075.
Acknowledgments
No acknowledgements.
Conflicts of Interest
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
References
- Benoit, R.M.; Baschat, A.A. Twin-to-twin transfusion syndrome: Prenatal diagnosis and treatment. Am. J. Perinatol. 2014, 31, 583–594. [Google Scholar] [CrossRef] [PubMed]
- Wohlmuth, C.; Gardiner, H.M.; Diehl, W.; Hecher, K. Fetal cardiovascular hemodynamics in twin-twin transfusion syndrome. Acta. Obstet. Gynecol. Scand. 2016, 95, 664–671. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Berghella, V.; Kaufmann, M. Natural history of twin-twin transfusion syndrome. J. Reprod. Med. 2001, 46, 480–484. [Google Scholar] [PubMed]
- Van Klink, J.M.; Koopman, H.M.; Rijken, M.; Middeldorp, J.M.; Oepkes, D.; Lopriore, E. Long-Term Neurodevelopmental Outcome in Survivors of Twin-to-Twin Transfusion Syndrome. Twin Res. Hum. Genet. 2016, 19, 255–261. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Slaghekke, F.; Lopriore, E.; Lewi, L.; Middeldorp, J.M.; van Zwet, E.W.; Weingertner, A.S.; Klumper, F.J.; DeKoninck, P.; Devlieger, R.; Kilby, M.D.; et al. Fetoscopic laser coagulation of the vascular equator versus selective coagulation for twin-to-twin transfusion syndrome: An open-label randomised controlled trial. Lancet 2014, 383, 2144–2151. [Google Scholar] [CrossRef]
- Slaghekke, F.; Oepkes, D. Solomon Technique Versus Selective Coagulation for Twin-Twin Transfusion Syndrome. Twin Res. Hum. Genet. 2016, 19, 217–221. [Google Scholar] [CrossRef] [PubMed]
- Lewi, L.; Cannie, M.; Blickstein, I.; Jani, J.; Huber, A.; Hecher, K.; Dymarkowski, S.; Gratacos, E.; Lewi, P.; Deprest, J. Placental sharing, birthweight discordance, and vascular anastomoses in monochorionic diamniotic twin placentas. Am. J. Obstet. Gynecol. 2007, 197, 587.e1–587.e8. [Google Scholar] [CrossRef]
- Khalil, A.; Beune, I.; Hecher, K.; Wynia, K.; Ganzevoort, W.; Reed, K.; Lewi, L.; Oepkes, D.; Gratacos, E.; Thilaganathan, B.; et al. Consensus definition and essential reporting parameters of selective fetal growth restriction in twin pregnancy: A Delphi procedure. Ultrasound Obstet. Gynecol. 2019, 53, 47–54. [Google Scholar] [CrossRef]
- Lopriore, E.; Sluimers, C.; Pasman, S.A.; Middeldorp, J.M.; Oepkes, D.; Walther, F.J. Neonatal morbidity in growth-discordant monochorionic twins: Comparison between the larger and the smaller twin. Twin Res. Hum. Genet. 2012, 15, 541–546. [Google Scholar] [CrossRef]
- Adegbite, A.L.; Castille, S.; Ward, S.; Bajoria, R. Neuromorbidity in preterm twins in relation to chorionicity and discordant birth weight. Am. J. Obstet. Gynecol. 2004, 190, 156–163. [Google Scholar] [CrossRef]
- Swamy, R.S.; McConachie, H.; Ng, J.; Rankin, J.; Korada, M.; Sturgiss, S.; Embleton, N.D. Cognitive outcome in childhood of birth weight discordant monochorionic twins: The long-term effects of fetal growth restriction. Arch. Dis. Child. Fetal Neonatal Ed. 2018, 103, F512–F516. [Google Scholar] [CrossRef] [PubMed]
- Rustico, M.A.; Consonni, D.; Lanna, M.; Faiola, S.; Schena, V.; Scelsa, B.; Introvini, P.; Righini, A.; Parazzini, C.; Lista, G.; et al. Selective intrauterine growth restriction in monochorionic twins: Changing patterns in umbilical artery Doppler flow and outcomes. Ultrasound Obstet. Gynecol. 2017, 49, 387–393. [Google Scholar] [CrossRef] [PubMed]
- Van Winden, K.R.; Quintero, R.A.; Kontopoulos, E.V.; Korst, L.M.; Llanes, A.; Chmait, R.H. Perinatal survival in cases of twin-twin transfusion syndrome complicated by selective intrauterine growth restriction. J. Matern. Fetal Neonatal Med. 2015, 28, 1549–1553. [Google Scholar] [CrossRef] [PubMed]
- Senat, M.V.; Deprest, J.; Boulvain, M.; Paupe, A.; Winer, N.; Ville, Y. Endoscopic laser surgery versus serial amnioreduction for severe twin-to-twin transfusion syndrome. N. Engl. J. Med. 2004, 351, 136–144. [Google Scholar] [CrossRef] [PubMed]
- Quintero, R.A.; Morales, W.J.; Allen, M.H.; Bornick, P.W.; Johnson, P.K.; Kruger, M. Staging of twin-twin transfusion syndrome. J. Perinatol. 1999, 19, 550–555. [Google Scholar] [CrossRef]
- Tollenaar, L.S.A.; Lopriore, E.; Middeldorp, J.M.; Haak, M.C.; Klumper, F.J.; Oepkes, D.; Slaghekke, F. Improved antenatal prediction of twin anemia-polycythemia sequence by delta middle cerebral artery peak systolic velocity: A new antenatal classification system. Ultrasound Obstet. Gynecol. 2018, 53, 788–793. [Google Scholar] [CrossRef] [PubMed]
- Lopriore, E.; Slaghekke, F.; Middeldorp, J.M.; Klumper, F.J.; van Lith, J.M.; Walther, F.J.; Oepkes, D. Accurate and simple evaluation of vascular anastomoses in monochorionic placenta using colored dye. J. Vis. Exp. 2011, 55, e3208. [Google Scholar] [CrossRef]
- Di Salvo, D.N.; Benson, C.B.; Laing, F.C.; Brown, D.L.; Frates, M.C.; Doubilet, P.M. Sonographic evaluation of the placental cord insertion site. AJR Am. J. Roentgenol. 1998, 170, 1295–1298. [Google Scholar] [CrossRef]
- Palisano, R.; Rosenbaum, P.; Walter, S.; Russell, D.; Wood, E.; Galuppi, B. Development and reliability of a system to classify gross motor function in children with cerebral palsy. Dev. Med. Child Neurol. 1997, 39, 214–223. [Google Scholar] [CrossRef]
- Bell, M.J.; Ternberg, J.L.; Feigin, R.D.; Keating, J.P.; Marshall, R.; Barton, L.; Brotherton, T. Neonatal necrotizing enterocolitis. Therapeutic decisions based upon clinical staging. Ann. Surg. 1978, 187, 1–7. [Google Scholar] [CrossRef]
- De Vries, L.S.; Eken, P.; Dubowitz, L.M. The spectrum of leukomalacia using cranial ultrasound. Behav. Brain Res. 1992, 49, 1–6. [Google Scholar] [CrossRef]
- Volpe, J.J. Intraventricular hemorrhage and brain injury in the premature infant. Diagnosis, prognosis, and prevention. Clin. Perinatol. 1989, 16, 387–411. [Google Scholar] [CrossRef]
- Levene, M.I. Measurement of the growth of the lateral ventricles in preterm infants with real-time ultrasound. Arch. Dis. Child. 1981, 56, 900–904. [Google Scholar] [CrossRef] [PubMed]
- Groene, S.G.; Tollenaar, L.S.A.; Slaghekke, F.; Middeldorp, J.M.; Haak, M.; Oepkes, D.; Lopriore, E. Placental characteristics in monochorionic twins with selective intrauterine growth restriction in relation to the umbilical artery Doppler classification. Placenta 2018, 71, 1–5. [Google Scholar] [CrossRef] [PubMed]
- Chmait, R.H.; Chon, A.H.; Schrager, S.M.; Kontopoulos, E.V.; Quintero, R.A.; Vanderbilt, D.L. Donor catch-up growth after laser surgery for twin-twin transfusion syndrome. Early Hum. Dev. 2015, 91, 751–754. [Google Scholar] [CrossRef] [PubMed]
- Eschbach, S.J.; Boons, L.S.; Wolterbeek, R.; Middeldorp, J.M.; Klumper, F.J.; Lopriore, E.; Oepkes, D.; Haak, M.C. Prediction of single fetal demise after laser therapy for twin-twin transfusion syndrome. Ultrasound Obstet. Gynecol. 2016, 47, 356–362. [Google Scholar] [CrossRef] [PubMed]
Figure 1.
Flowchart of study inclusion. TTTS: Twin-twin transfusion syndrome, MC: Monochorionic, EFW: Estimated fetal weight, TAPS: Twin anemia-polycythemia sequence, sFGR: Selective fetal growth restriction.
Figure 1.
Flowchart of study inclusion. TTTS: Twin-twin transfusion syndrome, MC: Monochorionic, EFW: Estimated fetal weight, TAPS: Twin anemia-polycythemia sequence, sFGR: Selective fetal growth restriction.
Figure 2.
Pictures of injected placentas after birth, both treated with laser surgery, illustrating the placental share division of the individual fetuses: (a) Placenta of a TTTS-only case; (b) placenta of a TTTS + sFGR case.
Figure 2.
Pictures of injected placentas after birth, both treated with laser surgery, illustrating the placental share division of the individual fetuses: (a) Placenta of a TTTS-only case; (b) placenta of a TTTS + sFGR case.
Figure 3.
Flowchart of available long-term follow-up for analysis.
Table 1.
Baseline maternal and neonatal characteristics for TTTS-only and TTTS + sFGR.
| Characteristics | TTTS-only (n = 430; 215 Pregnancies) | TTTS+sFGR (n = 624; 312 Pregnancies) | p-Value |
|---|---|---|---|
| Maternal age—years | 32.0 (29.0–35.0) | 31.0 (27.0–34.8) | 0.334 |
| Gravidity | 2 (1–3) | 2 (1–3) | 0.587 |
| Parity | 1 (0–1) | 1 (0–1) | 0.298 |
| Female | 196/424 (46) | 330/618 (53) | 0.023 |
| UA Doppler abnormalities | <0.0001 | ||
| Persistent A/REDF | 16/410 (4) | 73/592 (12) | |
| Intermittent A/REDF | 10/410 (2) | 24/592 (4) | |
| Quintero stage | <0.0001 | ||
| I | 39 (18) | 36 (12) | |
| II | 94 (44) | 87 (28) | |
| III | 77 (36) | 177 (57) | |
| IV | 5 (2) | 12 (4) | |
| Gestational age at birth—weeks | 33.1 (29.3–35.9) | 33.0 (29.7–35.7) | 0.780 |
| Caesarean | 150 (36) | 198 (33) | 0.262 |
| Birth weight—g | |||
| Donor | 1950 (1363–2415) | 1640 (1093–2125) | <0.0001 |
| Recipient | 2005 (1540–2475) | 1919 (1450–2303) | 0.136 |
| Birth weight discordance—% | 8.1 (3.3–13.7) | 15.6 (6.8–26.7) | <0.0001 |
| Birth weight discordance >25% | 10/161 (6) | 61/205 (30) | <0.0001 |
| Small for gestational age | 73/352 (21) | 231/465 (49) | <0.0001 |
| Donor is smaller twin at birth | 106/161 (66) | 172/206 (84) | <0.0001 |
Data are median (interquartile range (IQR)), n/N (%) or n (%); TTTS: Twin-twin transfusion syndrome, sFGR: Selective fetal growth restriction, UA: Umbilical artery, A/REDF: Absent/reversed end-diastolic flow.
Table 2.
Comparison of placental characteristics and laser surgery outcomes between TTTS-only and TTTS + sFGR cases.
Table 2.
Comparison of placental characteristics and laser surgery outcomes between TTTS-only and TTTS + sFGR cases.
| Characteristics | TTTS-only (n = 430; 215 Pregnancies) | TTTS + sFGR (n = 624; 312 Pregnancies) | p-Value |
|---|---|---|---|
| Gestational age at laser—weeks | 19.3 (17.4–22.0) | 20.0 (17.9–22.1) | 0.149 |
| Total anastomoses at fetoscopy—n | 6 (4–8) | 6 (4–7) | 0.412 |
| AV anastomoses—n | 4 (3–5) | 3 (3–4) | 0.176 |
| VA anastomoses—n | 2 (1–3) | 2 (1–3) | 0.822 |
| Presence of AA anastomoses—n/N (%) | 19/203 (9) | 51/296 (17) | 0.013 |
| Presence of VV anastomoses—n/N (%) | 19/203 (9) | 28/296 (10) | 0.970 |
| Residual anastomoses at birth—n/N (%) | 13/146 (9) | 18/209 (9) | 0.924 |
| Cord insertion—n/N (%) | |||
| Velamentous | 61/330 (19) | 116/440 (26) | 0.007 |
| Marginal | 63/330 (19) | 108/438 (25) | 0.083 |
| Placental share—% | |||
| Donor | 45.6 (41.2–50.7) | 42.6 (33.6–50.0) | 0.001 |
| Recipient | 54.4 (49.3–58.8) | 57.3 (50.0–66.4) | 0.002 |
| Placental share discordance—% | 22.1 (10.7–37.2) | 33.6 (18.1–52.0) | <0.0001 |
Data are median (interquartile ranges (IQR)), n/N (%) or n (%). TTTS: Twin-twin transfusion syndrome, sFGR: Selective fetal growth restriction, AV: Arterio-venous; VA: Veno-arterial; AA: Arterio-arterial, VV: Veno-venous.
Table 3.
Comparison of short- and long-term outcomes between TTTS-only and TTTS + sFGR cases.
| TTTS-only (n = 430; 215 Pregnancies) | TTTS + sFGR (n = 624; 312 Pregnancies) | p-Value | |
|---|---|---|---|
| Donor fetal death | 36 (17) | 76 (24) | 0.036 |
| Within 24h of laser | 9 (4) | 27 (9) | 0.404 |
| Recipient fetal death | 38 (18) | 68 (22) | 0.246 |
| Within 24h of laser | 13 (6) | 31 (10) | 0.377 |
| Severe neonatal morbidity | 66/287 (23) | 86/418 (21) | 0.593 |
| Donor | 32/144 (22) | 36/204 (18) | 0.289 |
| Recipient | 34/143 (24) | 50/214 (23) | 0.928 |
| Respiratory distress syndrome | 56/297 (19) | 71/429 (17) | 0.610 |
| Donor | 29/150 (19) | 29/209 (14) | 0.166 |
| Recipient | 27/147 (18) | 42/220 (19) | 0.862 |
| Patent ductus arteriosus | 14/295 (5) | 8/429 (2) | 0.100 |
| Donor | 6/149 (4) | 2/209 (1) | 0.053 |
| Recipient | 8/146 (6) | 6/220 (3) | 0.179 |
| Necrotizing enterocolitis | 9/292 (3) | 11/429 (3) | 0.770 |
| Donor | 4/147 (3) | 4/209 (2) | 0.613 |
| Recipient | 5/145 (3) | 7/220 (3) | 0.889 |
| Severe cerebral injury | 15/296 (5) | 30/439 (7) | 0.350 |
| Donor | 5/149 (3) | 14/213 (7) | 0.177 |
| Recipient | 10/147 (7) | 16/226 (7) | 0.918 |
| Neonatal mortality | 16/355 (5) | 14/478 (3) | 0.249 |
| Donor | 6/179 (3) | 11/235 (5) | 0.500 |
| Recipient | 10/176 (6) | 3/243 (1) | 0.010 |
| Perinatal survival | 339/429 (79) | 464/622 (75) | 0.176 |
| Donor | 173/215 (81) | 224/311 (72) | 0.027 |
| Recipient | 166/214 (78) | 240/311 (77) | 0.914 |
| Severe NDI at 2-year follow-up | 13/198 (7) | 27/299 (9) | 0.385 |
| Donor | 6/101 (6) | 13/150 (9) | 0.423 |
| Recipient | 7/97 (7) | 14/149 (9) | 0.550 |
| Disease-free survival | 185/288 (64) | 269/457 (59) | 0.176 |
| Donor | 95/143 (66) | 135/237 (57) | 0.067 |
| Recipient | 90/145 (62) | 134/220 (61) | 0.824 |
Data are n/N (%); TTTS: Twin-twin transfusion syndrome, sFGR: Selective fetal growth restriction, NDI: Neurodevelopmental impairment.
Table 4.
Analysis of potential risk factors for perinatal death after laser surgery in the TTTS + sFGR group.
Table 4.
Analysis of potential risk factors for perinatal death after laser surgery in the TTTS + sFGR group.
| Characteristics | Perinatal Survival (N = 464) | Perinatal Death (n = 158) | Univariate Analysis OR (95% CI) | SE | p-Value | Multivariate Analysis OR (95% CI) | SE | p-Value |
|---|---|---|---|---|---|---|---|---|
| Severe sFGR (EFW <3rd centile)—n/N (%) | 104/464 (22) | 40/158 (25) | 1.3 (0.9–1.9) | 0.197 | 0.197 | |||
| EFW discordance >25%—n/N (%) | 209/464 (45) | 67/158 (42) | 0.9 (0.6–1.4) | 0.217 | 0.620 | |||
| UA Dopplers prior to laser—n/N (%) | ||||||||
| Persistent A/REDF | 46/439 (11) | 27/151 (18) | 1.9 (1.1–3.1) | 0.263 | 0.018 | 1.7 (1.0–2.9) | 0.268 | 0.049 |
| Intermittent A/REDF | 18/439 (4) | 6/151 (4) | 1.0 (0.4–2.5) | 0.481 | 0.946 | |||
| Female—n/N (%) | 237/462 (51) | 93/154 (60) | 1.4 (0.9–2.2) | 0.218 | 0.090 | |||
| Quintero stage—n (%) | 0.847 | |||||||
| I | 50 (11) | 22 (14) | 0.8 (0.2–2.8) | 0.665 | ||||
| II | 132 (28) | 42 (27) | 1.0 (0.3–3.6) | 0.623 | ||||
| III | 264 (57) | 88 (56) | 1.0 (0.3–3.2) | 0.605 | ||||
| IV | 18 (4) | 6 (4) | - | - | ||||
| Gestational age at laser—weeks | 20.0 (18.0–22.4) | 19.8 (17.0–21.6) | 1.1 (1.0–1.2) | 0.033 | 0.008 | 1.1 (1.0–1.1) | 0.032 | 0.023 |
| Presence of AA anastomoses—n/N (%) | 36/222 (16) | 15/73 (21) | 1.3 (0.7–2.6) | 0.342 | 0.397 | |||
| Velamentous cord insertion—n/N (%) | 95/368 (26) | 19/70 (27) | 1.3 (0.8–2.0) | 0.227 | 0.264 |
Data are odds ratio (OR) (95% CI) and standard error (SE). OR: Odds ratio, CI: Confidence interval, SE: Standard error, sFGR: Selective fetal growth restriction, EFW: Estimated fetal weight, UA: Umbilical artery, A/REDF: Absent/reversed end-diastolic flow, AA: Arterio-arterial.
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Share and Cite
MDPI and ACS Style
Groene, S.G.; Tollenaar, L.S.A.; van Klink, J.M.M.; Haak, M.C.; Klumper, F.J.C.M.; Middeldorp, J.M.; Oepkes, D.; Slaghekke, F.; Lopriore, E. Twin-Twin Transfusion Syndrome with and without Selective Fetal Growth Restriction Prior to Fetoscopic Laser Surgery: Short and Long-Term Outcome. J. Clin. Med. 2019, 8, 969. https://doi.org/10.3390/jcm8070969
AMA Style
Groene SG, Tollenaar LSA, van Klink JMM, Haak MC, Klumper FJCM, Middeldorp JM, Oepkes D, Slaghekke F, Lopriore E. Twin-Twin Transfusion Syndrome with and without Selective Fetal Growth Restriction Prior to Fetoscopic Laser Surgery: Short and Long-Term Outcome. Journal of Clinical Medicine. 2019; 8(7):969. https://doi.org/10.3390/jcm8070969
Chicago/Turabian StyleGroene, Sophie G., Lisanne S. A. Tollenaar, Jeanine M. M. van Klink, Monique C. Haak, Frans J. C. M. Klumper, Johanna M. Middeldorp, Dick Oepkes, Femke Slaghekke, and Enrico Lopriore. 2019. "Twin-Twin Transfusion Syndrome with and without Selective Fetal Growth Restriction Prior to Fetoscopic Laser Surgery: Short and Long-Term Outcome" Journal of Clinical Medicine 8, no. 7: 969. https://doi.org/10.3390/jcm8070969
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.
