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Effects of Insulin on Porcine Neonatal Sertoli Cell Responsiveness to FSH In Vitro
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Effects of GH and IGF1 on Basal and FSH-Modulated Porcine Sertoli Cells In-Vitro

Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy
Department of Experimental Medicine, University of Perugia, 06132 Perugia, Italy
Department of Medicine, University of Perugia, 06132 Perugia, Italy
Author to whom correspondence should be addressed.
These authors contributed equally to this article.
These authors share the senior authorship of this article.
J. Clin. Med. 2019, 8(6), 811;
Received: 28 April 2019 / Revised: 24 May 2019 / Accepted: 5 June 2019 / Published: 6 June 2019
(This article belongs to the Special Issue Disorders of Puberty: The Causes and the Endocrine Medical Treatment)
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Several lines of evidence suggest that insulin-like growth factor 1 (IGF1) is involved in Sertoli cell (SC) proliferation and that its receptor (IGF1R) could mediate follicle-stimulating hormone (FSH) effects. To examine the role of the growth hormone (GH)-IGF1 axis on SC function, we evaluated the effects of GH and IGF1 on basal and FSH-modulated SC proliferation, as well as on anti-Müllerian hormone (AMH) and inhibin B expression and secretion in-vitro. SCs from neonatal pigs were incubated with (1) placebo, (2) 100 nM highly purified urofollitropin (hpFSH), (3) 100 nM recombinant GH (rGH), (4) 100 nM recombinant IGF1 (rIGF1), (5) 100 nM hpFSH plus 100 nM rGH, (6) 100 nM hpFSH plus 100 nM rIGF1, for 48 h. We found that IGF1, but not FSH nor GH, stimulated SC proliferation. Furthermore, an inhibitory effect of FSH, GH and IGF1 on AMH secretion, and a stimulatory role of FSH and IGF1, but not GH, on inhibin B secretion were found. These results suggest that the GH-IGF1 axis influences basal and FSH-modulated SC proliferation and function. We speculate that SC proliferation occurring in childhood might be supported by the increased serum IGF1 levels observed during this period of life. View Full-Text
Keywords: FSH; IGF1; GH; Sertoli cells; AMH FSH; IGF1; GH; Sertoli cells; AMH

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Cannarella, R.; Mancuso, F.; Condorelli, R.A.; Arato, I.; Mongioì, L.M.; Giacone, F.; Lilli, C.; Bellucci, C.; La Vignera, S.; Calafiore, R.; Luca, G.; Calogero, A.E. Effects of GH and IGF1 on Basal and FSH-Modulated Porcine Sertoli Cells In-Vitro. J. Clin. Med. 2019, 8, 811.

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