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Open AccessFeature PaperArticle

Surface Modification of Electrospun Scaffolds for Endothelialization of Tissue-Engineered Vascular Grafts Using Human Cord Blood-Derived Endothelial Cells

1
Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA
2
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21231, USA
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Sarver Heart Center, The University of Arizona, Tucson, AZ 85721, USA
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The Arizona Center for Accelerated BioMedical Innovation, University of Arizona, Tucson, AZ 85721, USA
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BIO5 Institute for Biocollaborative Research, The University of Arizona, Tucson, AZ 85721, USA
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Interventional Cardiology, University of Arizona, Tucson, AZ 85721, USA
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Arizona Health Science Center Biorepository, University of Arizona, Tucson, AZ 85724, USA
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McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA
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Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15219, USA
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Department of Immunobiology, Arizona Health Science Center Biorepository, University of Arizona, Tucson, AZ 85724, USA
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Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15219, USA
*
Author to whom correspondence should be addressed.
J. Clin. Med. 2019, 8(2), 185; https://doi.org/10.3390/jcm8020185
Received: 12 December 2018 / Revised: 22 January 2019 / Accepted: 1 February 2019 / Published: 4 February 2019
(This article belongs to the Special Issue Biobanking and Regenerative Medicine)
Tissue engineering has gained attention as an alternative approach for developing small diameter tissue-engineered vascular grafts intended for bypass surgery, as an option to treat coronary heart disease. To promote the formation of a healthy endothelial cell monolayer in the lumen of the graft, polycaprolactone/gelatin/fibrinogen scaffolds were developed, and the surface was modified using thermoforming and coating with collagen IV and fibronectin. Human cord blood-derived endothelial cells (hCB-ECs) were seeded onto the scaffolds and the important characteristics of a healthy endothelial cell layer were evaluated under static conditions using human umbilical vein endothelial cells as a control. We found that polycaprolactone/gelatin/fibrinogen scaffolds that were thermoformed and coated are the most suitable for endothelial cell growth. hCB-ECs can proliferate, produce endothelial nitric oxide synthase, respond to interleukin 1 beta, and reduce platelet deposition. View Full-Text
Keywords: Vascular tissue engineering; umbilical cord blood; human cord blood-derived endothelial cells; endothelialization; vascular graft Vascular tissue engineering; umbilical cord blood; human cord blood-derived endothelial cells; endothelialization; vascular graft
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MDPI and ACS Style

Ardila, D.C.; Liou, J.-J.; Maestas, D.; Slepian, M.J.; Badowski, M.; Wagner, W.R.; Harris, D.; Vande Geest, J.P. Surface Modification of Electrospun Scaffolds for Endothelialization of Tissue-Engineered Vascular Grafts Using Human Cord Blood-Derived Endothelial Cells. J. Clin. Med. 2019, 8, 185. https://doi.org/10.3390/jcm8020185

AMA Style

Ardila DC, Liou J-J, Maestas D, Slepian MJ, Badowski M, Wagner WR, Harris D, Vande Geest JP. Surface Modification of Electrospun Scaffolds for Endothelialization of Tissue-Engineered Vascular Grafts Using Human Cord Blood-Derived Endothelial Cells. Journal of Clinical Medicine. 2019; 8(2):185. https://doi.org/10.3390/jcm8020185

Chicago/Turabian Style

Ardila, Diana C.; Liou, Jr-Jiun; Maestas, David; Slepian, Marvin J.; Badowski, Michael; Wagner, William R.; Harris, David; Vande Geest, Jonathan P. 2019. "Surface Modification of Electrospun Scaffolds for Endothelialization of Tissue-Engineered Vascular Grafts Using Human Cord Blood-Derived Endothelial Cells" J. Clin. Med. 8, no. 2: 185. https://doi.org/10.3390/jcm8020185

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