Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice
by
Ana I. Sánchez-Garrido 1,2,†, Vanessa Prieto-Vicente 1,2,†, Víctor Blanco-Gozalo 3,†, Miguel Arévalo 2,4, Yaremi Quiros 3, Daniel López-Montañés 2,3, Francisco J. López-Hernández 2,5, Antonio Rodríguez-Pérez 1,2 and José M. López-Novoa 2,5,*
Ana I. Sánchez-Garrido 1,2,†, Vanessa Prieto-Vicente 1,2,†, Víctor Blanco-Gozalo 3,†, Miguel Arévalo 2,4, Yaremi Quiros 3, Daniel López-Montañés 2,3, Francisco J. López-Hernández 2,5, Antonio Rodríguez-Pérez 1,2 and José M. López-Novoa 2,5,*
1
Department of Gastroenterology, University Hospital of Salamanca, 37007 Salamanca, Spain
2
Institute for Biomedical Research of Salamanca (IBSAL), 37007 Salamanca, Spain.
3
Bio-inRen S.L. Faculty of Medicine, Campus Miguel de Unamuno, 37007 Salamanca, Spain
4
Department of Human Anatomy and Histology, University of Salamanca, 37007 Salamanca, Spain
5
Department of Physiology and Pharmacology, University of Salamanca, 37007 Salamanca, Spain
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work and share first authorship.
J. Clin. Med. 2019, 8(12), 2086; https://doi.org/10.3390/jcm8122086
Received: 10 October 2019 / Revised: 19 November 2019 / Accepted: 22 November 2019 / Published: 1 December 2019
(This article belongs to the Special Issue Ulcerative Colitis: Current and Emerging Treatment Strategies)
Ulcerative colitis is a relatively frequent, chronic disease that impacts significantly the patient’s quality of life. Although many therapeutic options are available, additional approaches are needed because many patients either do not respond to current therapies or show significant side effects. Cardiotrophin-1 (CT-1) is a cytokine with potent cytoprotective, anti-inflammatory, and antiapoptotic properties. The purpose of this study was to assess if the administration of CT-1 could reduce colon damage in mice with experimental colitis was induced with 5% dextran sulfate sodium (DSS) in the drinking water. Half of the mice received an i.v. dose of CT-1 (200 µg/kg) 2 h before and 2 and 4 days after DSS administration. Animals were followed during 7 days after DSS administration. The severity of colitis was measured by standard scores. Colon damage was assessed by histology and immunohistochemistry. Inflammatory mediators were measured by Western blot and PCR. CT-1 administration to DSS-treated mice ameliorated both the clinical course (disease activity index), histological damage, inflammation (colon expression of TNF-α, IL-17, IL-10, INF IFN-γ, and iNOS), and apoptosis. Our results suggest that CT-1 administration before induction of colitis improves the clinical course, tissue damage, and inflammation in DSS-induced colitis in mice.
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Keywords:
apoptosis; cardiotrophin-1; colon; inflammation
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MDPI and ACS Style
Sánchez-Garrido, A.I.; Prieto-Vicente, V.; Blanco-Gozalo, V.; Arévalo, M.; Quiros, Y.; López-Montañés, D.; López-Hernández, F.J.; Rodríguez-Pérez, A.; López-Novoa, J.M. Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice. J. Clin. Med. 2019, 8, 2086.
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