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J. Clin. Med. 2019, 8(1), 73; https://doi.org/10.3390/jcm8010073

Deduction of Novel Genes Potentially Involved in Keratinocytes of Type 2 Diabetes Using Next-Generation Sequencing and Bioinformatics Approaches

1
Department of Beauty Science, National Taichung University of Science and Technology, Taichung 403, Taiwan
2
Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
3
Division of Pulmonary and Critical Care Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
4
Department of Dermatology, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
5
Department of Physical Medicine and Rehabilitation, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
6
Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 7 December 2018 / Revised: 4 January 2019 / Accepted: 6 January 2019 / Published: 10 January 2019
(This article belongs to the Special Issue Type 2 Diabetes: Update on Pathophysiology and Treatment)
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Abstract

Keratinocytes constitute the major cell type of epidermis, which participates in re-epithelialization during wound repair and the immune defense response to pathogens. The aim of the current study was to explore the differentially expressed genes and novel microRNA (miRNA) regulations that are potentially involved in diabetic keratinocytes through next-generation sequencing (NGS) and bioinformatics approaches. A total of 420 differentially expressed genes between normal and diabetic keratinocytes were identified, and systematic bioinformatics analyses indicated that these differentially expressed genes were functionally enriched in interferon-alpha signaling, viral defense response, and immune response. Additionally, the potential miR-340-3p-DTX3L interaction that has been systematically validated in miRNA prediction databases was proposed to participate in the disrupted skin homeostasis, altering the defense and immune response of diabetic skin. The findings may provide new insights into understanding the pathogenesis of epidermal pathologies in diabetic patients and targeting novel molecules to advance diabetic skin care in clinical practice. View Full-Text
Keywords: type 2 diabetes; keratinocytes; immune response; next-generation sequencing; bioinformatics; microRNA; messenger RNA type 2 diabetes; keratinocytes; immune response; next-generation sequencing; bioinformatics; microRNA; messenger RNA
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Lin, E.-S.; Chang, W.-A.; Chen, Y.-Y.; Wu, L.-Y.; Chen, Y.-J.; Kuo, P.-L. Deduction of Novel Genes Potentially Involved in Keratinocytes of Type 2 Diabetes Using Next-Generation Sequencing and Bioinformatics Approaches. J. Clin. Med. 2019, 8, 73.

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