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Open AccessArticle

An Effective, Versatile, and Inexpensive Device for Oxygen Uptake Measurement

1
INSERM UMR 1141, Hôpital Robert Debré, 75019 Paris, France
2
Faculté de Médecine Denis Diderot, Université Paris Diderot—Paris 7, Site Robert Debré, 75013 Paris, France
3
MITOLOGICS S.A.S. Hôpital Robert Debré, 48 Bd Sérurier, 75019 Paris, France
*
Author to whom correspondence should be addressed.
Academic Editor: Iain P. Hargreaves
J. Clin. Med. 2017, 6(6), 58; https://doi.org/10.3390/jcm6060058
Received: 3 April 2017 / Revised: 19 May 2017 / Accepted: 6 June 2017 / Published: 8 June 2017
In the last ten years, the use of fluorescent probes developed to measure oxygen has resulted in several marketed devices, some unreasonably expensive and with little flexibility. We have explored the use of the effective, versatile, and inexpensive Redflash technology to determine oxygen uptake by a number of different biological samples using various layouts. This technology relies on the use of an optic fiber equipped at its tip with a membrane coated with a fluorescent dye (www.pyro-science.com). This oxygen-sensitive dye uses red light excitation and lifetime detection in the near infrared. So far, the use of this technology has mostly been used to determine oxygen concentration in open spaces for environmental studies, especially in aquatic media. The oxygen uptake determined by the device can be easily assessed in small volumes of respiration medium and combined with the measurement of additional parameters, such as lactate excretion by intact cells or the membrane potential of purified mitochondria. We conclude that the performance of by this technology should make it a first choice in the context of both fundamental studies and investigations for respiratory chain deficiencies in human samples. View Full-Text
Keywords: respiration assay; oxygen uptake; glycolysis; mitochondriopathy respiration assay; oxygen uptake; glycolysis; mitochondriopathy
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MDPI and ACS Style

Bénit, P.; Chrétien, D.; Porceddu, M.; Yanicostas, C.; Rak, M.; Rustin, P. An Effective, Versatile, and Inexpensive Device for Oxygen Uptake Measurement. J. Clin. Med. 2017, 6, 58.

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