MGMT Methylation Is Associated with Human Papillomavirus Infection in Cervical Dysplasia: A Longitudinal Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Specimens
2.2. DNA Extraction and Methylation Analysis
2.3. The Cancer Genome Atlas (TCGA) Data Analysis
3. Results
3.1. MGMT Methylation and HPV Infection Status in ASC-US/LSIL Patients
3.2. MGMT Methylation and mRNA Expression
3.3. MGMT as a Prognostic Biomarker
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Patient ID | Age | Sample Information | Cervical Cytology | HPV Types |
---|---|---|---|---|
Pt 1 | 32 | 1st collection | ASCUS | 18 |
2nd collection | NIL * | 18 | ||
Pt 2 | 29 | 1st collection | ASCUS | 33 |
2nd collection | ASCUS | 33 | ||
Pt 3 | 22 | 1st collection | ASCUS | 39 |
2nd collection | NIL * | 39 | ||
Pt 4 | 58 | 1st collection | ASCUS | 58 |
2nd collection | ASCUS | 58 | ||
Pt 5 | 49 | 1st collection | ASCUS | 58 |
2nd collection | ASCUS | 58 | ||
Pt 6 | 37 | 1st collection | LSIL | 52 |
2nd collection | LSIL | 52 | ||
Pt 7 | 41 | 1st collection | ASCUS | 52 |
2nd collection | NIL * | 52 |
Patient ID | Sample Information | Ct (Control) | Ct (MGMT) | ΔCt | MPR |
---|---|---|---|---|---|
Pt 1 | 1st collection | 27.29 | 36.82 | 9.53 | 0.14 |
2nd collection | 31.38 | 38.23 | 6.85 | 0.86 | |
Pt 2 | 1st collection | 27.93 | 35.41 | 7.48 | 0.56 |
2nd collection | 32.83 | 37.55 | 4.72 | 3.66 | |
Pt 3 | 1st collection | 31.06 | 36.58 | 5.52 | 2.13 |
2nd collection | 26.39 | 30.93 | 4.54 | 4.12 | |
Pt 4 | 1st collection | 24.21 | 32.39 | 8.18 | 0.34 |
2nd collection | 28.44 | 32.64 | 4.20 | 5.16 | |
Pt 5 | 1st collection | 23.21 | 30.06 | 6.85 | 0.86 |
2nd collection | 27.37 | 36.46 | 9.09 | 0.18 | |
Pt 6 | 1st collection | 27.47 | 34.75 | 7.28 | 0.64 |
2nd collection | 27.72 | 31.90 | 4.18 | 5.23 | |
Pt 7 | 1st collection | 21.74 | 30.05 | 8.31 | 0.31 |
2nd collection | 27.00 | 34.80 | 7.80 | 0.45 |
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Choi, B.; Na, Y.; Whang, M.Y.; Ho, J.Y.; Han, M.-R.; Park, S.-W.; Song, H.; Hur, S.Y.; Choi, Y.J. MGMT Methylation Is Associated with Human Papillomavirus Infection in Cervical Dysplasia: A Longitudinal Study. J. Clin. Med. 2023, 12, 6188. https://doi.org/10.3390/jcm12196188
Choi B, Na Y, Whang MY, Ho JY, Han M-R, Park S-W, Song H, Hur SY, Choi YJ. MGMT Methylation Is Associated with Human Papillomavirus Infection in Cervical Dysplasia: A Longitudinal Study. Journal of Clinical Medicine. 2023; 12(19):6188. https://doi.org/10.3390/jcm12196188
Chicago/Turabian StyleChoi, Boram, Yoojin Na, Min Yeop Whang, Jung Yoon Ho, Mi-Ryung Han, Seong-Woo Park, Heekyoung Song, Soo Young Hur, and Youn Jin Choi. 2023. "MGMT Methylation Is Associated with Human Papillomavirus Infection in Cervical Dysplasia: A Longitudinal Study" Journal of Clinical Medicine 12, no. 19: 6188. https://doi.org/10.3390/jcm12196188