Caspase-1 Inhibition Reduces Occurrence of PANoptosis in Macrophages Infected by E. faecalis OG1RF
Abstract
:1. Introduction
2. Materials and Methods
2.1. Culture of Enterococcus faecalis and Macrophages
2.2. RAW264.7 Cells Infected with E. faecalis
2.3. Real-Time Cell Death Analysis
2.4. Quantitative Real-time Polymerase Chain Reaction (RT-qPCR)
2.5. Western Blotting
2.6. Scanning Electron Microscopy
2.7. Statistical Analysis
3. Results
3.1. Dynamic Changes in Macrophages Infected with E. faecalis in a Dose-Dependent Manner
3.2. Protein Expression of Macrophages Infected by E. faecalis at Three MOIs
3.3. The Effect of Caspase-1 Inhibitor on Real-Time Cell Death of Macrophages
3.4. The Effect of Caspase-1 Inhibitor on Gene Expression of Two Cytokines
3.5. Protein Expression of E. faecalis-Infected Macrophages Treated with a Caspase-1 Inhibitor
3.6. Morphological Modification of E. faecalis-Infected Macrophages under Caspase-1 Inhibitor Treatment
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Chi, D.; Zhang, Y.; Lin, X.; Gong, Q.; Tong, Z. Caspase-1 Inhibition Reduces Occurrence of PANoptosis in Macrophages Infected by E. faecalis OG1RF. J. Clin. Med. 2022, 11, 6204. https://doi.org/10.3390/jcm11206204
Chi D, Zhang Y, Lin X, Gong Q, Tong Z. Caspase-1 Inhibition Reduces Occurrence of PANoptosis in Macrophages Infected by E. faecalis OG1RF. Journal of Clinical Medicine. 2022; 11(20):6204. https://doi.org/10.3390/jcm11206204
Chicago/Turabian StyleChi, Danlu, Yuejiao Zhang, Xinwei Lin, Qimei Gong, and Zhongchun Tong. 2022. "Caspase-1 Inhibition Reduces Occurrence of PANoptosis in Macrophages Infected by E. faecalis OG1RF" Journal of Clinical Medicine 11, no. 20: 6204. https://doi.org/10.3390/jcm11206204