Next Article in Journal
The Association between IgG and Disease Severity Parameters in CF Patients
Next Article in Special Issue
Prognostic Value of Trial Round Window Stimulation for Selection of Candidates for Cochlear Implantation as Treatment for Tinnitus
Previous Article in Journal
Balloon Enteroscopy-Assisted Endoscopic Retrograde Cholangiopancreatography for the Treatment of Common Bile Duct Stones in Patients with Roux-en-Y Gastrectomy: Outcomes and Factors Affecting Complete Stone Extraction
Previous Article in Special Issue
Rates, Indications, and Speech Perception Outcomes of Revision Cochlear Implantations
Article

Evaluation of the Efficacy of Dexamethasone-Eluting Electrode Array on the Post-Implant Cochlear Fibrotic Reaction by Three-Dimensional Immunofluorescence Analysis in Mongolian Gerbil Cochlea

1
Department of Otology and Neurotology, CHU Lille, University of Lille 2 Henri Warembourg, F-59000 Lille, France
2
INSERM U1008—Controlled Drug Delivery Systems and Biomaterials, F-59000 Lille, France
3
BioImaging Center Lille-Nord de France (BICeL), University of Lille 2 Henri Warembourg, F-59000 Lille, France
*
Author to whom correspondence should be addressed.
Academic Editor: Christof Röösli
J. Clin. Med. 2021, 10(15), 3315; https://doi.org/10.3390/jcm10153315
Received: 7 July 2021 / Revised: 22 July 2021 / Accepted: 26 July 2021 / Published: 28 July 2021
Cochlear implant is the method of choice for the rehabilitation of severe to profound sensorineural hearing loss. The study of the tissue response to cochlear implantation and the prevention of post-cochlear-implant damages are areas of interest in hearing protection research. The objective was to assess the efficacy of dexamethasone-eluting electrode array on endo canal fibrosis formation by three-dimensional immunofluorescence analysis in implanted Mongolian gerbil cochlea. Two trials were conducted after surgery using Mongolian gerbil implanted with dexamethasone-eluting or non-eluting intracochlear electrode arrays. The animals were then euthanised 10 weeks after implantation. The cochleae were prepared (electrode array in place) according to a 29-day protocol with immunofluorescent labelling and tissue clearing. The acquisition was carried out using light-sheet microscopy. Imaris software was then used for three-dimensional analysis of the cochleae and quantification of the fibrotic volume. The analysis of 12 cochleae showed a significantly different mean volume of fibrosis (2.16 × 108 μm3 ± 0.15 in the dexamethasone eluting group versus 3.17 × 108 μm3 ± 0.54 in the non-eluting group) (p = 0.004). The cochlear implant used as a corticosteroid delivery system appears to be an encouraging device for the protection of the inner ear against fibrosis induced by implantation. Three-dimensional analysis of the cochlea by light-sheet microscopy was suitable for studying post-implantation tissue damage. View Full-Text
Keywords: auditory implant; dexamethasone; hearing loss; rehabilitation; clearing; imaging; light-sheet microscopy auditory implant; dexamethasone; hearing loss; rehabilitation; clearing; imaging; light-sheet microscopy
Show Figures

Figure 1

MDPI and ACS Style

Toulemonde, P.; Risoud, M.; Lemesre, P.E.; Beck, C.; Wattelet, J.; Tardivel, M.; Siepmann, J.; Vincent, C. Evaluation of the Efficacy of Dexamethasone-Eluting Electrode Array on the Post-Implant Cochlear Fibrotic Reaction by Three-Dimensional Immunofluorescence Analysis in Mongolian Gerbil Cochlea. J. Clin. Med. 2021, 10, 3315. https://doi.org/10.3390/jcm10153315

AMA Style

Toulemonde P, Risoud M, Lemesre PE, Beck C, Wattelet J, Tardivel M, Siepmann J, Vincent C. Evaluation of the Efficacy of Dexamethasone-Eluting Electrode Array on the Post-Implant Cochlear Fibrotic Reaction by Three-Dimensional Immunofluorescence Analysis in Mongolian Gerbil Cochlea. Journal of Clinical Medicine. 2021; 10(15):3315. https://doi.org/10.3390/jcm10153315

Chicago/Turabian Style

Toulemonde, Philippine, Michaël Risoud, Pierre E. Lemesre, Cyril Beck, Jean Wattelet, Meryem Tardivel, Juergen Siepmann, and Christophe Vincent. 2021. "Evaluation of the Efficacy of Dexamethasone-Eluting Electrode Array on the Post-Implant Cochlear Fibrotic Reaction by Three-Dimensional Immunofluorescence Analysis in Mongolian Gerbil Cochlea" Journal of Clinical Medicine 10, no. 15: 3315. https://doi.org/10.3390/jcm10153315

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop