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Open AccessArticle

Membrane Adsorber for the Fast Purification of a Monoclonal Antibody Using Protein A Chromatography

1
Institute of Technical Chemistry, Callinstraße 5, 30167 Hannover, Germany
2
Sartorius Stedim Biotech, August-Spindler-Straße 11, 37079 Göttingen, Germany
*
Author to whom correspondence should be addressed.
Membranes 2019, 9(12), 159; https://doi.org/10.3390/membranes9120159
Received: 24 September 2019 / Revised: 21 November 2019 / Accepted: 25 November 2019 / Published: 27 November 2019
(This article belongs to the Special Issue Membrane Chromatography for Biomolecules Purification)
Monoclonal antibodies are conquering the biopharmaceutical market because they can be used to treat a variety of diseases. Therefore, it is very important to establish robust and optimized processes for their production. In this article, the first step of chromatography (Protein A chromatography) in monoclonal antibody purification was optimized with a focus on the critical elution step. Therefore, different buffers (citrate, glycine, acetate) were tested for chromatographic performance and product quality. Membrane chromatography was evaluated because it promises high throughputs and short cycle times. The membrane adsorber Sartobind® Protein A 2 mL was used to accelerate the purification procedure and was further used to perform a continuous chromatographic run with a four-membrane adsorber-periodic counter-current chromatography (4MA-PCCC) system. It was found that citrate buffer at pH 3.5 and 0.15 M NaCl enabled the highest recovery of >95% and lowest total aggregate content of 0.26%. In the continuous process, the capacity utilization of the membrane adsorber was increased by 20%. View Full-Text
Keywords: monoclonal antibody; membrane adsorber; protein A chromatography; periodic counter-current chromatography monoclonal antibody; membrane adsorber; protein A chromatography; periodic counter-current chromatography
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MDPI and ACS Style

Brämer, C.; Tünnermann, L.; Gonzalez Salcedo, A.; Reif, O.-W.; Solle, D.; Scheper, T.; Beutel, S. Membrane Adsorber for the Fast Purification of a Monoclonal Antibody Using Protein A Chromatography. Membranes 2019, 9, 159.

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