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Polymer-Induced Swelling of Solid-Supported Lipid Membranes

1
Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Hahn-Meitner-Platz 1, Berlin 14109, Germany
2
Angewandte Physikalische Chemie, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 253, Heidelberg 69120, Germany
*
Author to whom correspondence should be addressed.
Present Address: Catalan Institute of Nanoscience and Nanotechnology (ICN2), Bellaterra (Barcelona) 08193, Spain
Academic Editor: Maikel Rheinstadter
Membranes 2016, 6(1), 2; https://doi.org/10.3390/membranes6010002
Received: 12 September 2015 / Revised: 4 December 2015 / Accepted: 15 December 2015 / Published: 23 December 2015
(This article belongs to the Special Issue Membrane Structure and Dynamics)
In this paper, we study the interaction of charged polymers with solid-supported 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes by in-situ neutron reflectivity. We observe an enormous swelling of the oligolamellar lipid bilayer stacks after incubation in solutions of poly(allylamine hydrochloride) (PAH) in D2O. The positively charged polyelectrolyte molecules interact with the lipid bilayers and induce a drastic increase in their d-spacing by a factor of ~4. Temperature, time, and pH influence the swollen interfacial lipid linings. From our study, we conclude that electrostatic interactions introduced by the adsorbed PAH are the main cause for the drastic swelling of the lipid coatings. The DMPC membrane stacks do not detach from their solid support at T > Tm. Steric interactions, also introduced by the PAH molecules, are held responsible for the stabilizing effect. We believe that this novel system offers great potential for fundamental studies of biomembrane properties, keeping the membrane’s natural fluidity and freedom, decoupled from a solid support at physiological conditions. View Full-Text
Keywords: lipid; membranes; polymer; neutron reflectivity lipid; membranes; polymer; neutron reflectivity
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MDPI and ACS Style

Kreuzer, M.; Trapp, M.; Dahint, R.; Steitz, R. Polymer-Induced Swelling of Solid-Supported Lipid Membranes. Membranes 2016, 6, 2.

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