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Membranes
Volume 11
Issue 7
10.3390/membranes11070495
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Article

AFM Study of Nanoscale Membrane Perturbation Induced by Antimicrobial Lipopeptide C14 KYR

by
Sawinee Nasompag
1,
Pawinee Siritongsuk
2,
Saengrawee Thammawithan
2,
Oranee Srichaiyapol
2,
Panchika Prangkio
3,
Terri A. Camesano
4,
Chomdao Sinthuvanich
1,5 and
Rina Patramanon
2,*
1
Interdisciplinary Graduate Program in Genetic Engineering, The Graduate School, Kasetsart University, Bangkok 10900, Thailand
2
Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
3
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
4
Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA
5
Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
*
Author to whom correspondence should be addressed.
Membranes 2021, 11(7), 495; https://doi.org/10.3390/membranes11070495
Submission received: 26 May 2021 / Revised: 23 June 2021 / Accepted: 28 June 2021 / Published: 30 June 2021
(This article belongs to the Section Biological Membrane Composition and Structures)
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Abstract

Lipopeptides have been extensively studied as potential antimicrobial agents. In this study, we focused on the C14-KYR lipopeptide, a modified version of the KYR tripeptide with myristic acid at the N-terminus. Here, membrane perturbation of live E. coli treated with the parent KYR and C14-KYR peptides was compared at the nanoscale level using AFM imaging. AFM analyses, including average cellular roughness and force spectroscopy, revealed the severe surface disruption mechanism of C14-KYR. A loss of surface roughness and changes in topographic features included membrane shrinkage, periplasmic membrane separation from the cell wall, and cytosolic leakage. Additional evidence from synchrotron radiation FTIR microspectroscopy (SR-FTIR) revealed a marked structural change in the membrane component after lipopeptide attack. The average roughness of the E. coli cell before and after treatment with C14-KYR was 129.2 ± 51.4 and 223.5 ± 14.1 nm, respectively. The average rupture force of the cell treated with C14-KYR was 0.16 nN, four times higher than that of the untreated cell. Our study demonstrates that the mechanistic effect of the lipopeptide against bacterial cells can be quantified through surface imaging and adhesion force using AFM.
Keywords: lipopeptide; rupture force; nanoscale; live bacterial cell; membrane lipopeptide; rupture force; nanoscale; live bacterial cell; membrane

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MDPI and ACS Style

Nasompag, S.; Siritongsuk, P.; Thammawithan, S.; Srichaiyapol, O.; Prangkio, P.; Camesano, T.A.; Sinthuvanich, C.; Patramanon, R. AFM Study of Nanoscale Membrane Perturbation Induced by Antimicrobial Lipopeptide C14 KYR. Membranes 2021, 11, 495. https://doi.org/10.3390/membranes11070495

AMA Style

Nasompag S, Siritongsuk P, Thammawithan S, Srichaiyapol O, Prangkio P, Camesano TA, Sinthuvanich C, Patramanon R. AFM Study of Nanoscale Membrane Perturbation Induced by Antimicrobial Lipopeptide C14 KYR. Membranes. 2021; 11(7):495. https://doi.org/10.3390/membranes11070495

Chicago/Turabian Style

Nasompag, Sawinee, Pawinee Siritongsuk, Saengrawee Thammawithan, Oranee Srichaiyapol, Panchika Prangkio, Terri A. Camesano, Chomdao Sinthuvanich, and Rina Patramanon. 2021. "AFM Study of Nanoscale Membrane Perturbation Induced by Antimicrobial Lipopeptide C14 KYR" Membranes 11, no. 7: 495. https://doi.org/10.3390/membranes11070495

APA Style

Nasompag, S., Siritongsuk, P., Thammawithan, S., Srichaiyapol, O., Prangkio, P., Camesano, T. A., Sinthuvanich, C., & Patramanon, R. (2021). AFM Study of Nanoscale Membrane Perturbation Induced by Antimicrobial Lipopeptide C14 KYR. Membranes, 11(7), 495. https://doi.org/10.3390/membranes11070495

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