Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Characterization
2.2. CDs and CDs-DOX Preparation
2.3. Quantum Yield Measurement
2.4. Cell Imaging
2.5. FCS Setup
2.6. Statistical Analysis
3. Results
3.1. Synthesis and Characterization of CDs, DOX and CDs-DOX
3.2. Investigating Nanodrug Transmembrane Transport
3.3. Investigating Nanodrug Transmembrane Transport under Different Conditions
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Time (min) | 5 min | 6 min | 7 min | 8 min | |
---|---|---|---|---|---|
Drug Diffusion Coefficient (×10−6 cm2 s−1) | CDs | 5.91 ± 1.29 (*) | 6.412 ± 1.25 (*) | 7.32 ± 0.81 (**) | 6.95 ± 1.89 (*) |
DOX | 7.90 ± 1.15 (*) | 5.5 ± 0.563 (***) | 5.94 ± 0.32 (***) | 5.94 ± 1.98 (*) | |
CDs-DOX | 69.58 ± 9.48 (**) | 65.41 ± 10.9 (**) | 46.72 ± 10.5 (*) | 32.7 ± 10.9 (*) |
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Gao, X.; Liu, Y.; Zhang, J.; Wang, L.; Guo, Y.; Zhu, Y.; Yang, Z.; Yan, W.; Qu, J. Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy. Membranes 2021, 11, 891. https://doi.org/10.3390/membranes11110891
Gao X, Liu Y, Zhang J, Wang L, Guo Y, Zhu Y, Yang Z, Yan W, Qu J. Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy. Membranes. 2021; 11(11):891. https://doi.org/10.3390/membranes11110891
Chicago/Turabian StyleGao, Xinwei, Yanfeng Liu, Jia Zhang, Luwei Wang, Yong Guo, Yinru Zhu, Zhigang Yang, Wei Yan, and Junle Qu. 2021. "Nanodrug Transmembrane Transport Research Based on Fluorescence Correlation Spectroscopy" Membranes 11, no. 11: 891. https://doi.org/10.3390/membranes11110891