Next Article in Journal
Chemically Crosslinked Sulfonated Polyphenylsulfone (CSPPSU) Membranes for PEM Fuel Cells
Next Article in Special Issue
Effect of Vitamin K3 Inhibiting the Function of NorA Efflux Pump and Its Gene Expression on Staphylococcus aureus
Previous Article in Journal
Comparison of Supported Ionic Liquid Membranes and Polymeric Ultrafiltration and Nanofiltration Membranes for Separation of Lignin and Monosaccharides
Open AccessBrief Report

Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure

MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK
Science for Life Laboratory, Department of Women’s and Children’s Health, Karolinska Institutet, 171 65 Stockholm, Sweden
Bioengineering Science Research Group, Faculty of Engineering and Physical Sciences, Institute for Life Sciences (IfLS), University of Southampton, SO17 1BJ Southampton, UK
McGovern Medical School, Department of Integrative Biology and Pharmacology, University of Texas Health Science Center at Houston, Houston, TX 77030, USA
Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford OX3 7DQ, UK
Institute of Applied Optics and Biophysics, Friedrich-Schiller-University Jena, Max-Wien Platz 4, 07743 Jena, Germany
Leibniz Institute of Photonic Technology e.V., Albert-Einstein-Straße 9, 07745 Jena, Germany
Authors to whom correspondence should be addressed.
Membranes 2020, 10(2), 30;
Received: 23 January 2020 / Revised: 12 February 2020 / Accepted: 14 February 2020 / Published: 18 February 2020
(This article belongs to the Special Issue Dynamics and Nano-Organization in Plasma Membranes)
Model membrane systems are essential tools for the study of biological processes in a simplified setting to reveal the underlying physicochemical principles. As cell-derived membrane systems, giant plasma membrane vesicles (GPMVs) constitute an intermediate model between live cells and fully artificial structures. Certain applications, however, require planar membrane surfaces. Here, we report a new approach for creating supported plasma membrane bilayers (SPMBs) by bursting cell-derived GPMVs using ultrasound within a microfluidic device. We show that the mobility of outer leaflet molecules is preserved in SPMBs, suggesting that they are accessible on the surface of the bilayers. Such model membrane systems are potentially useful in many applications requiring detailed characterization of plasma membrane dynamics. View Full-Text
Keywords: GPMVs; acoustic pressure; supported bilayers; plasma membrane vesicles; plasma membrane bilayers GPMVs; acoustic pressure; supported bilayers; plasma membrane vesicles; plasma membrane bilayers
Show Figures

Figure 1

MDPI and ACS Style

Sezgin, E.; Carugo, D.; Levental, I.; Stride, E.; Eggeling, C. Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure. Membranes 2020, 10, 30.

AMA Style

Sezgin E, Carugo D, Levental I, Stride E, Eggeling C. Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure. Membranes. 2020; 10(2):30.

Chicago/Turabian Style

Sezgin, Erdinc; Carugo, Dario; Levental, Ilya; Stride, Eleanor; Eggeling, Christian. 2020. "Creating Supported Plasma Membrane Bilayers Using Acoustic Pressure" Membranes 10, no. 2: 30.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop