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Vaccines
Volume 9
Issue 6
10.3390/vaccines9060613
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Article

Optimization of Vero Cells Grown on a Polymer Fiber Carrier in a Disposable Bioreactor for Inactivated Coxsackievirus A16 Vaccine Development

by
Keda Chen
1,
Chaonan Li
1,†,
Ying Wang
1,†,
Zhenwei Shen
1,
Yikai Guo
1,
Xiaoping Li
1 and
Yanjun Zhang
2,*
1
Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China
2
Department of Virus Inspection, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China
*
Author to whom correspondence should be addressed.
†
Co-first authors.
Vaccines 2021, 9(6), 613; https://doi.org/10.3390/vaccines9060613
Submission received: 9 April 2021 / Revised: 2 June 2021 / Accepted: 3 June 2021 / Published: 7 June 2021
(This article belongs to the Special Issue Analysis and Modification of Existing Vaccines for the Development of Next-Generation Vaccines)
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Abstract

At present, there are no vaccines available for hand, foot, and mouth disease, which is caused by Coxsackie virus A16 (CVA16) infection. In the present study, we isolated epidemic strains of CVA16 and optimized the production of the virus in Vero cells. The system comprised growing the infected cells on polymer fiber paper carriers in a serum-free medium containing 0.5% (w/v) lactalbumin hydrolysate a mini bioreactor. Disposable Bioflo310 and AmProtein Current perfusion bioreactors were used to monitor virus infection and Vero cell culture. The total number of cells increased from 1.5 × 109 to 3.0 × 1010. In our optimized culture process, the virus titer reached 7.8 × 107 TCID50/mL at three days after infection. The inactivated CVA16 prepared from our optimized culture procedure elicited a slightly higher neutralizing antibody titer compared with that derived from routine culture procedures. These results will promote the large-scale production of inactivated CVA16 vaccines using nonwoven polymer fiber paper cell cultures.
Keywords: coxsackie virus A16; polymer fiber paper carriers; disposable mini bioreactor; vaccine coxsackie virus A16; polymer fiber paper carriers; disposable mini bioreactor; vaccine

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MDPI and ACS Style

Chen, K.; Li, C.; Wang, Y.; Shen, Z.; Guo, Y.; Li, X.; Zhang, Y. Optimization of Vero Cells Grown on a Polymer Fiber Carrier in a Disposable Bioreactor for Inactivated Coxsackievirus A16 Vaccine Development. Vaccines 2021, 9, 613. https://doi.org/10.3390/vaccines9060613

AMA Style

Chen K, Li C, Wang Y, Shen Z, Guo Y, Li X, Zhang Y. Optimization of Vero Cells Grown on a Polymer Fiber Carrier in a Disposable Bioreactor for Inactivated Coxsackievirus A16 Vaccine Development. Vaccines. 2021; 9(6):613. https://doi.org/10.3390/vaccines9060613

Chicago/Turabian Style

Chen, Keda, Chaonan Li, Ying Wang, Zhenwei Shen, Yikai Guo, Xiaoping Li, and Yanjun Zhang. 2021. "Optimization of Vero Cells Grown on a Polymer Fiber Carrier in a Disposable Bioreactor for Inactivated Coxsackievirus A16 Vaccine Development" Vaccines 9, no. 6: 613. https://doi.org/10.3390/vaccines9060613

APA Style

Chen, K., Li, C., Wang, Y., Shen, Z., Guo, Y., Li, X., & Zhang, Y. (2021). Optimization of Vero Cells Grown on a Polymer Fiber Carrier in a Disposable Bioreactor for Inactivated Coxsackievirus A16 Vaccine Development. Vaccines, 9(6), 613. https://doi.org/10.3390/vaccines9060613

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