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Volume 9
Issue 5
10.3390/vaccines9050442
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Article

Differential Antibody Response against Conformational and Linear Epitopes of the L1 Proteins from Human Papillomavirus Types 16/18 Is Observed in Vaccinated Women or with Uterine Cervical Lesions

by
Adolfo Pedroza-Saavedra
1,
Angelica Nallelhy Rodriguez-Ocampo
2,
Azucena Salazar-Piña
3,
Aislinn Citlali Perez-Morales
1,4,
Lilia Chihu-Amparan
1,
Minerva Maldonado-Gama
1,
Aurelio Cruz-Valdez
5,
Fernando Esquivel-Guadarrama
4 and
Lourdes Gutierrez-Xicotencatl
1,*
1
Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, 62100 Cuernavaca, Mexico
2
Unidad Académica Químico Biológicas y Ciencias Farmacéuticas, Universidad Autónoma de Nayarit, 36715 Tepic, Mexico
3
Facultad de Nutrición, Universidad Autónoma del Estado de Morelos, 62100 Cuernavaca, Mexico
4
Facultad de Medicina, Universidad Autónoma del Estado de Morelos, 62100 Cuernavaca, Mexico
5
Centro de Investigación en Salud Poblacional, Instituto Nacional de Salud Pública, 62100 Cuernavaca, Mexico
*
Author to whom correspondence should be addressed.
Vaccines 2021, 9(5), 442; https://doi.org/10.3390/vaccines9050442
Submission received: 23 March 2021 / Revised: 19 April 2021 / Accepted: 23 April 2021 / Published: 2 May 2021
(This article belongs to the Special Issue Development of Vaccines Based on Virus-Like Particles)
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Abstract

Antibodies against the Human Papillomavirus (HPV) L1 protein are associated with past infections and related to the evolution of the disease, whereas antibodies against L1 Virus-Like Particles (VLPs) are used to follow the neutralizing antibody response in vaccinated women. In this study, serum antibodies against conformational (VLPs) and linear epitopes of HPV16/18 L1 protein were assessed to distinguish HPV-vaccinated women from those naturally infected or those with uterine cervical lesions. The VLPs-16/18 were generated in baculovirus, and L1 proteins were obtained from denatured VLPs. Serum antibodies against VLPs and L1 proteins were evaluated by ELISA. The ELISA-VLPs and ELISA-L1 16/18 assays were validated with a vaccinated women group by ROC analysis and the regression analysis to distinguish the different populations of female patients. The anti-VLPs-16/18 and anti-L1-16/18 antibodies effectively detect vaccinated women (AUC = 1.0/0.79, and 0.94/0.84, respectively). The regression analysis showed that anti-VLPs-16/18 and anti-L1-16/18 antibodies were associated with the vaccinated group (OR = 2.11 × 108/16.50 and 536.0/49.2, respectively). However, only the anti-L1-16 antibodies were associated with the high-grade lesions and cervical cancer (CIN3/CC) group (OR = 12.18). In conclusion, our results suggest that anti-VLPs-16/18 antibodies are effective and type-specific to detect HPV-vaccinated women, but anti-L1-16 antibodies better differentiate the CIN3/CC group. However, a larger population study is needed to validate these results.
Keywords: cervical cancer; human papillomavirus; L1 protein; VLPs; linear and conformational epitopes; HPV vaccine cervical cancer; human papillomavirus; L1 protein; VLPs; linear and conformational epitopes; HPV vaccine

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MDPI and ACS Style

Pedroza-Saavedra, A.; Rodriguez-Ocampo, A.N.; Salazar-Piña, A.; Perez-Morales, A.C.; Chihu-Amparan, L.; Maldonado-Gama, M.; Cruz-Valdez, A.; Esquivel-Guadarrama, F.; Gutierrez-Xicotencatl, L. Differential Antibody Response against Conformational and Linear Epitopes of the L1 Proteins from Human Papillomavirus Types 16/18 Is Observed in Vaccinated Women or with Uterine Cervical Lesions. Vaccines 2021, 9, 442. https://doi.org/10.3390/vaccines9050442

AMA Style

Pedroza-Saavedra A, Rodriguez-Ocampo AN, Salazar-Piña A, Perez-Morales AC, Chihu-Amparan L, Maldonado-Gama M, Cruz-Valdez A, Esquivel-Guadarrama F, Gutierrez-Xicotencatl L. Differential Antibody Response against Conformational and Linear Epitopes of the L1 Proteins from Human Papillomavirus Types 16/18 Is Observed in Vaccinated Women or with Uterine Cervical Lesions. Vaccines. 2021; 9(5):442. https://doi.org/10.3390/vaccines9050442

Chicago/Turabian Style

Pedroza-Saavedra, Adolfo, Angelica Nallelhy Rodriguez-Ocampo, Azucena Salazar-Piña, Aislinn Citlali Perez-Morales, Lilia Chihu-Amparan, Minerva Maldonado-Gama, Aurelio Cruz-Valdez, Fernando Esquivel-Guadarrama, and Lourdes Gutierrez-Xicotencatl. 2021. "Differential Antibody Response against Conformational and Linear Epitopes of the L1 Proteins from Human Papillomavirus Types 16/18 Is Observed in Vaccinated Women or with Uterine Cervical Lesions" Vaccines 9, no. 5: 442. https://doi.org/10.3390/vaccines9050442

APA Style

Pedroza-Saavedra, A., Rodriguez-Ocampo, A. N., Salazar-Piña, A., Perez-Morales, A. C., Chihu-Amparan, L., Maldonado-Gama, M., Cruz-Valdez, A., Esquivel-Guadarrama, F., & Gutierrez-Xicotencatl, L. (2021). Differential Antibody Response against Conformational and Linear Epitopes of the L1 Proteins from Human Papillomavirus Types 16/18 Is Observed in Vaccinated Women or with Uterine Cervical Lesions. Vaccines, 9(5), 442. https://doi.org/10.3390/vaccines9050442

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