Protective Potential of an Autogenous Vaccine in an Aerogenous Model of Escherichia coli Infection in Broiler Breeders
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals, Housing and Experimental Design
2.2. Inoculum
2.3. E. coli Aerosol-Challenge
2.4. Euthanasia and Gross Pathology
2.5. Microbiology
2.6. Histopathology
2.7. Ethics
2.8. Statistics
3. Results
3.1. Exposure Levels and Inoculation Conditions
3.2. Clinical Signs
3.3. Gross Pathology
3.4. Histopathology
3.5. Microbiology
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Vaccinated-Challenged (n = 20) | Not Vaccinated-Challenged (n = 10) | Vaccinated-Not Challenged (n = 10) | Not Vaccinated-Not Challenged (n = 7) | |
---|---|---|---|---|
Lung/BW-ratio | 3 × 10−3 ± 5.5 × 10−4 * | 2.7 × 10−3 ± 2.7 × 10−4 | 2.5 10−3 ± 6.2 × 10−4 | 2.7 × 10−3 ± 1.6 × 10−3 |
Liver/BW-ratio | 2.4 × 10−2 ± 3.9 × 10−3 | 2.3 × 10−2 ± 3.7 × 10−3 | 2.2 × 10−2 ± 2.8 × 10−3 | 2.3 × 10−2 ± 3.8 × 10−3 |
Spleen/BW-ratio | 9.2 × 10−4 ± 1.8 × 10−4 | 8.9 × 10−4 ± 1.7 × 10−4 | 7.6 × 10−4 ± 1.6 × 10−4 | 8.2 × 10−4 ± 1.5 × 10−4 |
Appendix B
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Vaccinated-Challenged (n = 20) | Not Vaccinated-Challenged (n = 10) | Vaccinated-Not Challenged (n = 10) | Not Vaccinated-Not Challenged (n = 7) | |
---|---|---|---|---|
In-lay, euthanasia 1 | 18/20 (90%) | 10/10 (100%) | 9/10 (90%) | 7/7 (100%) |
Tracheal changes (hyperaemia, and/or mucoid, purulent and/or fibrinous tracheitis) | 7/20 (35%) | 5/10 (50%) | 2/10 (20%) | 0/7 (0%) |
Pulmonary changes (consolidation, oedema and/or exudate) | 13/20 (65%) | 8/10 (80%) | 3/10 (30%) | 3/7 (42%) |
Airsacculitis (hyperaemia, opaqueness and/or exudate) | 10/20 (50%) | 8/10 (80%) | 2/10 (20%) | 2/7 (28%) |
Pericarditis (purulent and/or fibrinous) | 0/20 (0%) | 1/10 (10%) | 0/10 (0%) | 0/7 (0%) |
Perihepatitis (purulent and/or fibrinous) | 0/20 (0%) | 0/10 (0%) | 0/10 (0%) | 0/7 (0%) |
Peritonitis (purulent and/or fibrinous) | 4/20 (20%) | 3/10 (30%) | 1/10 (10%) | 1/7 (14%) |
Perioophoritis (purulent and/or fibrinous) | 5/20 (25%) | 3/10 (30%) | 1/10 (10%) | 1/7 (14%) |
Overall lesion score | 1.95 | 2.8 | 0.9 | 1.0 |
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Kromann, S.; Olsen, R.H.; Bojesen, A.M.; Jensen, H.E.; Thøfner, I. Protective Potential of an Autogenous Vaccine in an Aerogenous Model of Escherichia coli Infection in Broiler Breeders. Vaccines 2021, 9, 1233. https://doi.org/10.3390/vaccines9111233
Kromann S, Olsen RH, Bojesen AM, Jensen HE, Thøfner I. Protective Potential of an Autogenous Vaccine in an Aerogenous Model of Escherichia coli Infection in Broiler Breeders. Vaccines. 2021; 9(11):1233. https://doi.org/10.3390/vaccines9111233
Chicago/Turabian StyleKromann, Sofie, Rikke Heidemann Olsen, Anders Miki Bojesen, Henrik Elvang Jensen, and Ida Thøfner. 2021. "Protective Potential of an Autogenous Vaccine in an Aerogenous Model of Escherichia coli Infection in Broiler Breeders" Vaccines 9, no. 11: 1233. https://doi.org/10.3390/vaccines9111233
APA StyleKromann, S., Olsen, R. H., Bojesen, A. M., Jensen, H. E., & Thøfner, I. (2021). Protective Potential of an Autogenous Vaccine in an Aerogenous Model of Escherichia coli Infection in Broiler Breeders. Vaccines, 9(11), 1233. https://doi.org/10.3390/vaccines9111233