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Article

Molecular Differentiation of Mycoplasma gallisepticum Outbreaks: A Last Decade Study on Italian Farms Using GTS and MLST

1
Mycoplasma Unit—SCT1, Istituto Zooprofilattico Sperimentale delle Venezie, Via San Giacomo 5, 37135 Verona, Italy
2
Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungária körút 21, 1143 Budapest, Hungary
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(4), 665; https://doi.org/10.3390/vaccines8040665
Received: 9 October 2020 / Revised: 5 November 2020 / Accepted: 5 November 2020 / Published: 9 November 2020
(This article belongs to the Special Issue Poultry Vaccines)
Mycoplasma gallisepticum (MG) infects many avian species and leads to significant economic losses in the poultry industry. Transmission of this pathogen occurs both horizontally and vertically, and strategies to avoid the spread of MG rely on vaccination and the application of biosecurity measures to maintain breeder groups as pathogen-free. Two live attenuated MG vaccine strains are licensed in Italy: 6/85 and ts-11. After their introduction, the implementation of adequate genotyping tools became necessary to distinguish between field and vaccine strains and to guarantee proper infection monitoring activity. In this study, 40 Italian MG isolates collected between 2010–2019 from both vaccinated and unvaccinated farms were genotyped using gene-targeted sequencing (GTS) of the cythadesin gene mgc2 and multilocus sequence typing (MLST) based on six housekeeping genes. The discriminatory power of GTS typing ensures 6/85-like strain identification, but the technique does not allow the identification ts-11 strains; conversely, MLST differentiates both vaccine strains, describing more detailed interrelation structures. Our study describes MG genetic scenario within a mixed farming context. In conclusion, the use of adequate typing methods is essential to understand the evolutionary dynamics of MG strains in a particular area and to conduct epidemiological investigations in the avian population. View Full-Text
Keywords: Mycoplasma gallisepticum; GTS; MLST; vaccine; poultry Mycoplasma gallisepticum; GTS; MLST; vaccine; poultry
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MDPI and ACS Style

Matucci, A.; Stefani, E.; Gastaldelli, M.; Rossi, I.; De Grandi, G.; Gyuranecz, M.; Catania, S. Molecular Differentiation of Mycoplasma gallisepticum Outbreaks: A Last Decade Study on Italian Farms Using GTS and MLST. Vaccines 2020, 8, 665. https://doi.org/10.3390/vaccines8040665

AMA Style

Matucci A, Stefani E, Gastaldelli M, Rossi I, De Grandi G, Gyuranecz M, Catania S. Molecular Differentiation of Mycoplasma gallisepticum Outbreaks: A Last Decade Study on Italian Farms Using GTS and MLST. Vaccines. 2020; 8(4):665. https://doi.org/10.3390/vaccines8040665

Chicago/Turabian Style

Matucci, Andrea, Elisabetta Stefani, Michele Gastaldelli, Ilenia Rossi, Gelinda De Grandi, Miklós Gyuranecz, and Salvatore Catania. 2020. "Molecular Differentiation of Mycoplasma gallisepticum Outbreaks: A Last Decade Study on Italian Farms Using GTS and MLST" Vaccines 8, no. 4: 665. https://doi.org/10.3390/vaccines8040665

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