Recombinant Fowlpox Virus Expressing gB Gene from Predominantly Epidemic Infectious Larygnotracheitis Virus Strain Demonstrates Better Immune Protection in SPF Chickens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statements
2.2. Plasmid, Viruses, Cells, gB Protein, and Experimental Animals
2.3. Sequence Analysis of I6E1 and WG Strain
2.4. Construction of rFPV-gB
2.5. Characterization of rFPV-gB
2.6. Immunization and Challenge Study
2.6.1. Challenge Efficacy of ILTVs
Amplification and Determination of EID50 of ILTVs
Challenge Efficacy of ILTVs in SPF Chickens
2.6.2. Protective Efficacy of rFPV-gB against ILTVs in SPF Chickens
2.7. Statistical Analysis
3. Results
3.1. Sequence Analysis of I6E1 and WG Strain
3.2. Construction of rFPV of ILTV gB Gene
3.3. Characterization and Expression of ILTV gB Gene in rFPV
3.4. Virus Growth Curve
3.5. Challenge Study of ILTVs in SPF Chickens
3.5.1. Clinical Signs and Throat Tracheal Lesions of Chickens after Challenge
3.5.2. The Clinical Index, Morbidity, and Mortality of I1E1, I6E1, WG, and I19
3.6. Protection Induced by Immunization with rFPV-gB
3.6.1. Antibody Responses to ILTV after Vaccination
3.6.2. Clinical Signs and Laryngotracheal Lesions of Chickens after Immunization with rFPV-gB against ILTV Strain I19
3.6.3. The Clinical Index, Morbidity, and Mortality of Chickens after Immunization with rFPV-gB against ILTV Strain I19
3.6.4. Protection Percentage of SPF Chickens by Immunization with rFPV-gB
3.6.5. Virus Shedding
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Class | TK (%) | ICP4 | ORFB-TK | gB | ||||
---|---|---|---|---|---|---|---|---|
WG | I6E1 | WG | I6E1 | WG | I6E1 | WG | I6E1 | |
1 | 100 | 100 | 99.6 | 99.6 | 99.9 | 99.7 | 99.7 | 99.8 |
2 | 99.5 | 99.5 | 99.7 | 99.7 | 99.7 | 99.6 | 99.7 | 99.8 |
3 | 99.5 | 99.5 | 99.6 | 99.6 | 99.7 | 99.6 | 99.7 | 99.8 |
4 | 100 | 100 | 100 | 100 | 100 | 99.7 | 100 | 100 |
5 | 100 | 100 | 99.6 | 99.6 | 99.9 | 99.7 | 99.7 | 99.8 |
6 | 99.5 | 99.5 | 99.7 | 99.7 | 99.7 | 99.6 | 99.7 | 99.8 |
7 | 99.6 | 99.6 | 99.9 | 99.8 | 99.7 | 99.7 | 99.9 | 99.9 |
8 | 100 | 100 | 100 | 100 | 99.7 | 100 | 100 | 100 |
9 | 100 | 100 | 99.9 | 99.9 | 99.9 | 99.7 | 99.9 | 99.9 |
10 | 100 | 100 | 99.6 | 99.6 | 99.7 | 99.5 | 99.7 | 99.6 |
Group a | Dose | Challenge Route | Clinical Index (Total Score/Total Chickens) b | Morbidity (Number of Cases/Total Chickens) c | Mortality (Number of Deaths/Total Chickens) c |
---|---|---|---|---|---|
I1E1 | 105 EID50/per | intraocular-nasal route | 0.75 (3/4) | 75% (3/4) | 0 (0/4) |
I6E1 | 105 EID50/per | intraocular-nasal route | 0.75 (3/4) | 75% (3/4) | 0 (0/4) |
WG | 105 EID50/per | intraocular-nasal route | 1.5 (6/4) | 100% (4/4) | 0 (0/4) |
106 EID50/per | oropharyngeal route | 3 (12/4) | 100% (4/4) | 100% (4/4) | |
I19 | 105 EID50/per | intraocular-nasal route | 1.5 (6/4) | 100% (4/4) | 0 (0/4) |
106 EID50/per | oropharyngeal route | 2.5 (10/4) | 100% (4/4) | 50% (2/4) |
Immunization Group a. | Clinical Index (Total Score/Total Chickens) b | Morbidity (Number of Cases/Total Chickens) c | Mortality (Number of Deaths/Total Chickens) c |
---|---|---|---|
Commercial rFPV vaccine | 0.6 (6/10) | 40% (4/10) a | 10% (1/10) |
rFPV-gB | 0.3 (3/10) | 30% (3/10) a | 0 (0/10) |
wt-FPV | 2.5 (25/10) | 100% (10/10) b | 60% (6/10) |
PBS | 2.4 (24/10) | 100% (10/10) | 60% (6/10) |
Immunization Group | Immunization Dose | Mortality (Number of Deaths/Total Chickens) | Protection Index (PI) |
---|---|---|---|
Commercial rFPV vaccine | 105 PFU c/0.2 mL | 1/10 (10%) b | 83.3 |
rFPV-gB | 105 PFU/0.2 mL | 0/10 (0) a | 100 |
wt-FPV | 105 PFU/0.2 mL | 6/10 (60%) b | 0 |
PBS | 0.2 mL | 6/10 (60%) | 0 |
Immunization Group | I19 Virus Isolation Rate (Positive Number/Total Number) | ||
---|---|---|---|
3 d.p.i. a | 5 d.p.i. | 7 d.p.i. | |
Commercial rFPV vaccine | 90% b (9/10) c | 100% (9/9) | 100% (9/9) |
rFPV282E4-gB | 70% (7/10) | 100% (10/10) | 100% (10/10) |
wt-FPV282E4 | 100% (10/10) | 100% (4/4) | 100% (4/4) |
PBS | 100% (10/10) | 100% (7/7) | 100% (4/4) |
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Chen, S.; Xu, N.; Ta, L.; Li, S.; Su, X.; Xue, J.; Du, Y.; Qin, T.; Peng, D. Recombinant Fowlpox Virus Expressing gB Gene from Predominantly Epidemic Infectious Larygnotracheitis Virus Strain Demonstrates Better Immune Protection in SPF Chickens. Vaccines 2020, 8, 623. https://doi.org/10.3390/vaccines8040623
Chen S, Xu N, Ta L, Li S, Su X, Xue J, Du Y, Qin T, Peng D. Recombinant Fowlpox Virus Expressing gB Gene from Predominantly Epidemic Infectious Larygnotracheitis Virus Strain Demonstrates Better Immune Protection in SPF Chickens. Vaccines. 2020; 8(4):623. https://doi.org/10.3390/vaccines8040623
Chicago/Turabian StyleChen, Sujuan, Nuo Xu, Lei Ta, Shi Li, Xiang Su, Jing Xue, Yinping Du, Tao Qin, and Daxin Peng. 2020. "Recombinant Fowlpox Virus Expressing gB Gene from Predominantly Epidemic Infectious Larygnotracheitis Virus Strain Demonstrates Better Immune Protection in SPF Chickens" Vaccines 8, no. 4: 623. https://doi.org/10.3390/vaccines8040623