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Volume 8
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10.3390/vaccines8030551
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Article

Solid Lipid Nanoparticle Carrier Platform Containing Synthetic TLR4 Agonist Mediates Non-Viral DNA Vaccine Delivery

by
Jasmine E. Francis
1,
Ivana Skakic
1,
Chaitali Dekiwadia
2,
Ravi Shukla
3,
Aya C. Taki
4,
Anna Walduck
1 and
Peter M. Smooker
1,*
1
School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia
2
RMIT Microscopy & Microanalysis Facility, School of Science, RMIT University, Melbourne, VIC 3001, Australia
3
NanoBiotechnology Research Laboratory, School of Science, RMIT University, Melbourne, VIC 3001, Australia
4
Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(3), 551; https://doi.org/10.3390/vaccines8030551
Submission received: 26 August 2020 / Revised: 14 September 2020 / Accepted: 16 September 2020 / Published: 21 September 2020
(This article belongs to the Special Issue Vaccines for Infectious and Chronic Diseases)
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Abstract

There is a growing demand for better delivery systems to improve the stability and efficacy of DNA vaccines. Here we report the synthesis of a non-viral DNA vaccine delivery system using a novel adjuvanted solid lipid nanoparticle (SLN-A) platform as a carrier for a DNA vaccine candidate encoding the Urease alpha (UreA) antigen from Helicobacter pylori. Cationic SLN-A particles containing monophosphoryl lipid A (adjuvant) were synthesised by a modified solvent-emulsification method and were investigated for their morphology, zeta potential and in vitro transfection capacity. Particles were found to bind plasmid DNA to form lipoplexes, which were characterised by electron microscopy, dynamic light scattering and fluorescence microscopy. Cellular uptake studies confirmed particle uptake within 3 h, and intracellular localisation within endosomal compartments. In vitro studies further confirmed the ability of SLN-A particles to stimulate expression of pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α) in human macrophage-like Tohoku Hospital Pediatrics-1 (THP-1) cells. Lipoplexes were found to be biocompatible and could be efficiently transfected in murine immune cells for expression of recombinant H. pylori antigen Urease A, demonstrating their potential as a DNA vaccine delivery system.
Keywords: DNA vaccine; solid lipid nanoparticle; nanobiotechnology; Helicobacter pylori; lipoplex DNA vaccine; solid lipid nanoparticle; nanobiotechnology; Helicobacter pylori; lipoplex

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MDPI and ACS Style

Francis, J.E.; Skakic, I.; Dekiwadia, C.; Shukla, R.; Taki, A.C.; Walduck, A.; Smooker, P.M. Solid Lipid Nanoparticle Carrier Platform Containing Synthetic TLR4 Agonist Mediates Non-Viral DNA Vaccine Delivery. Vaccines 2020, 8, 551. https://doi.org/10.3390/vaccines8030551

AMA Style

Francis JE, Skakic I, Dekiwadia C, Shukla R, Taki AC, Walduck A, Smooker PM. Solid Lipid Nanoparticle Carrier Platform Containing Synthetic TLR4 Agonist Mediates Non-Viral DNA Vaccine Delivery. Vaccines. 2020; 8(3):551. https://doi.org/10.3390/vaccines8030551

Chicago/Turabian Style

Francis, Jasmine E., Ivana Skakic, Chaitali Dekiwadia, Ravi Shukla, Aya C. Taki, Anna Walduck, and Peter M. Smooker. 2020. "Solid Lipid Nanoparticle Carrier Platform Containing Synthetic TLR4 Agonist Mediates Non-Viral DNA Vaccine Delivery" Vaccines 8, no. 3: 551. https://doi.org/10.3390/vaccines8030551

APA Style

Francis, J. E., Skakic, I., Dekiwadia, C., Shukla, R., Taki, A. C., Walduck, A., & Smooker, P. M. (2020). Solid Lipid Nanoparticle Carrier Platform Containing Synthetic TLR4 Agonist Mediates Non-Viral DNA Vaccine Delivery. Vaccines, 8(3), 551. https://doi.org/10.3390/vaccines8030551

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