Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Cell Infection and Virus Quantification
2.3. mRNA Relative Quantification
2.4. Type-I IFN Bioassay
2.5. Statistical Analysis
3. Results
3.1. SeV Infection Is Highly Efficient in Ovine Cells
3.2. SeV Infection Induced Stable GFP Expression in Ovine Cells
3.3. SeV Infection Induces Proinflammatory Responses in Ovine Cells
3.4. SeV-Infected Cells Reduced Permissibility to SRLV Infection
3.5. Ovine Cells Infected with SeV-GFP Inhibit HIV-1-GFP Vector Infectivity
3.6. Restriction Factors Induced after SeV Infection in Ovine Cells
3.7. SeV Infection May Induce Local Resistance to SRLV
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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de Pablo-Maiso, L.; Echeverría, I.; Rius-Rocabert, S.; Luján, L.; Garcin, D.; de Andrés, D.; Nistal-Villán, E.; Reina, R. Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells. Vaccines 2020, 8, 206. https://doi.org/10.3390/vaccines8020206
de Pablo-Maiso L, Echeverría I, Rius-Rocabert S, Luján L, Garcin D, de Andrés D, Nistal-Villán E, Reina R. Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells. Vaccines. 2020; 8(2):206. https://doi.org/10.3390/vaccines8020206
Chicago/Turabian Stylede Pablo-Maiso, Lorena, Irache Echeverría, Sergio Rius-Rocabert, Lluís Luján, Dominique Garcin, Damián de Andrés, Estanislao Nistal-Villán, and Ramsés Reina. 2020. "Sendai Virus, a Strong Inducer of Anti-Lentiviral State in Ovine Cells" Vaccines 8, no. 2: 206. https://doi.org/10.3390/vaccines8020206