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Vaccines
Volume 8
Issue 1
10.3390/vaccines8010121
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Article

A Novel Peptide Isolated from a Phage Display Peptide Library Modeling Antigenic Epitope of DHAV-1 and DHAV-3

by
Ruihua Zhang
1,2,3,
Yupeng Yang
2,3,
Jingjing Lan
2,3,
Shaoli Lin
2,
Zhijing Xie
2,3,
Xiansheng Zhang
1 and
Shijin Jiang
2,3,*
1
College of Life Science, Shandong Agricultural University, Taian 271018, China
2
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China
3
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian 271018, China
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(1), 121; https://doi.org/10.3390/vaccines8010121
Received: 25 February 2020 / Accepted: 4 March 2020 / Published: 5 March 2020
(This article belongs to the Section Veterinary Vaccines)
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Abstract

Duck hepatitis A virus (DHAV), the major pathogen of duck virus hepatitis (DVH), causes severe diseases that threaten the duck industry worldwide. The VP1 protein, a major structural protein of DHAV, is able to induce neutralizing antibody in ducks. The purpose of this study was to identify the antigenic mimotope of DHAV by phage display technology. A monoclonal antibody (mAb) 4E6 against DHAV-1 and DHAV-3 was prepared, and a phage library prepared with the PhD-12 Phage Display Peptide Library Kit was screened with the mAb. A novel peptide, 1GLTWKLPPSM10 was identified with high affinity to the mAb and could specifically block mAb 4E6 from binding DHAV-1 and DHAV-3. Animal tests confirmed that the immunization of ducklings with the mimotope could inhibit the virus proliferation and protect the ducklings from DVH. In summary, the neutralizing conformational mimotope 1GLTWKLPPSM10 might be a promising vaccine candidate for the prevention of DHAV infection. View Full-Text
Keywords: duck hepatitis A virus; monoclonal antibody; phage display; mimitope; immunization duck hepatitis A virus; monoclonal antibody; phage display; mimitope; immunization
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MDPI and ACS Style

Zhang, R.; Yang, Y.; Lan, J.; Lin, S.; Xie, Z.; Zhang, X.; Jiang, S. A Novel Peptide Isolated from a Phage Display Peptide Library Modeling Antigenic Epitope of DHAV-1 and DHAV-3. Vaccines 2020, 8, 121. https://doi.org/10.3390/vaccines8010121

AMA Style

Zhang R, Yang Y, Lan J, Lin S, Xie Z, Zhang X, Jiang S. A Novel Peptide Isolated from a Phage Display Peptide Library Modeling Antigenic Epitope of DHAV-1 and DHAV-3. Vaccines. 2020; 8(1):121. https://doi.org/10.3390/vaccines8010121

Chicago/Turabian Style

Zhang, Ruihua, Yupeng Yang, Jingjing Lan, Shaoli Lin, Zhijing Xie, Xiansheng Zhang, and Shijin Jiang. 2020. "A Novel Peptide Isolated from a Phage Display Peptide Library Modeling Antigenic Epitope of DHAV-1 and DHAV-3" Vaccines 8, no. 1: 121. https://doi.org/10.3390/vaccines8010121

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