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Article

Yellow Fever Virus Genotyping Tool and Investigation of Suspected Adverse Events Following Yellow Fever Vaccination

by
Izabela Maurício de Rezende
1,
Pedro Augusto Alves
2,
Matheus Soares Arruda
1,
Andreza Parreiras Gonçalves
2,
Gabriela Fernanda Garcia Oliveira
1,
Leonardo Soares Pereira
1,3,
Maria Rita Teixeira Dutra
3,
Ana Carolina Campi-Azevedo
2,
Valéria Valim
4,
Renata Tourinho
5,
Jaquelline Germano de Oliveira
2,
Carlos Eduardo Calzavara
2,
Rodrigo Fabiano do Carmo Said
6,7,
Erna Geessien Kroon
1,
Olindo Assis Martins-Filho
2,
Andrea Teixeira-Carvalho
2 and
Betânia Paiva Drumond
1,*
1
Departament of Microbiology, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Minas Gerais, Brazil
2
Instituto René Rachou/Fundação Oswaldo Cruz, 30190-002 Belo Horizonte, Minas Gerais, Brazil
3
Hospital Eduardo de Menezes, Fundação Hospitalar do Estado de Minas Gerais, 30622-020 Belo Horizonte, Minas Gerais, Brazil
4
Hospital Universitário Cassiano Antônio Moraes - Empresa Brasileira de Serviços Hospitalares, Universidade Federal do Espírito Santo, 29075-910 Vitória, Espírito Santo, Brazil
5
Biomanguinhos/Fundação Oswaldo Cruz, 21040-900 Rio de Janeiro, Rio de Janeiro, Brazil
6
Secretaria de Estado de Saúde de Minas Gerais, 31630-900 Belo Horizonte, Minas Gerais, Brazil
7
Departamento de Vigilância das Doenças Transmissíveis, Secretaria de Vigilância em Saúde, Ministério da Saúde, 70058-900 Brasília, Distrito Federal, Brazil
*
Author to whom correspondence should be addressed.
Vaccines 2019, 7(4), 206; https://doi.org/10.3390/vaccines7040206
Submission received: 30 October 2019 / Revised: 28 November 2019 / Accepted: 29 November 2019 / Published: 4 December 2019
(This article belongs to the Special Issue Arbovirus Vaccines That Circulate within the Same Ecological Niche: Zika, Dengue & Chikungunya)
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Abstract

The yellow fever (YF) vaccine consists of an attenuated virus, and despite its relative safety, some adverse events following YF vaccination have been described. At the end of 2016, Brazil experienced the most massive sylvatic yellow fever outbreak over the last 70 years and an intense campaign of YF vaccination occurred in Minas Gerais state in Southeast Brazil from 2016 to 2018. The present study aimed to develop a genotyping tool and investigate 21 cases of suspected adverse events following YF vaccination. Initial in silico analyses were performed using partial NS5 nucleotide sequences to verify the discriminatory potential between wild-type and vaccine viruses. Samples from patients were screened for the presence of the YFV RNA, using 5′UTR as the target, and then used for amplification of partial NS5 gene amplification, sequencing, and phylogenetic analysis. Genotyping indicated that 17 suspected cases were infected by the wild-type yellow fever virus, but four cases remained inconclusive. The genotyping tool was efficient in distinguishing the vaccine from wild-type virus, and it has the potential to be used for the differentiation of all yellow fever virus genotypes.
Keywords: yellow fever virus; YFV-17DD; adverse event; arbovirus; yellow fever; vaccination; immunisation; vaccine; genotyping; wild-type virus yellow fever virus; YFV-17DD; adverse event; arbovirus; yellow fever; vaccination; immunisation; vaccine; genotyping; wild-type virus

Share and Cite

MDPI and ACS Style

Rezende, I.M.d.; Alves, P.A.; Arruda, M.S.; Gonçalves, A.P.; Oliveira, G.F.G.; Pereira, L.S.; Dutra, M.R.T.; Campi-Azevedo, A.C.; Valim, V.; Tourinho, R.; et al. Yellow Fever Virus Genotyping Tool and Investigation of Suspected Adverse Events Following Yellow Fever Vaccination. Vaccines 2019, 7, 206. https://doi.org/10.3390/vaccines7040206

AMA Style

Rezende IMd, Alves PA, Arruda MS, Gonçalves AP, Oliveira GFG, Pereira LS, Dutra MRT, Campi-Azevedo AC, Valim V, Tourinho R, et al. Yellow Fever Virus Genotyping Tool and Investigation of Suspected Adverse Events Following Yellow Fever Vaccination. Vaccines. 2019; 7(4):206. https://doi.org/10.3390/vaccines7040206

Chicago/Turabian Style

Rezende, Izabela Maurício de, Pedro Augusto Alves, Matheus Soares Arruda, Andreza Parreiras Gonçalves, Gabriela Fernanda Garcia Oliveira, Leonardo Soares Pereira, Maria Rita Teixeira Dutra, Ana Carolina Campi-Azevedo, Valéria Valim, Renata Tourinho, and et al. 2019. "Yellow Fever Virus Genotyping Tool and Investigation of Suspected Adverse Events Following Yellow Fever Vaccination" Vaccines 7, no. 4: 206. https://doi.org/10.3390/vaccines7040206

APA Style

Rezende, I. M. d., Alves, P. A., Arruda, M. S., Gonçalves, A. P., Oliveira, G. F. G., Pereira, L. S., Dutra, M. R. T., Campi-Azevedo, A. C., Valim, V., Tourinho, R., Oliveira, J. G. d., Calzavara, C. E., Said, R. F. d. C., Kroon, E. G., Martins-Filho, O. A., Teixeira-Carvalho, A., & Drumond, B. P. (2019). Yellow Fever Virus Genotyping Tool and Investigation of Suspected Adverse Events Following Yellow Fever Vaccination. Vaccines, 7(4), 206. https://doi.org/10.3390/vaccines7040206

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