Integrated Transcriptomic and Proteomic Analysis of Red Blood Cells from Rainbow Trout Challenged with VHSV Point Towards Novel Immunomodulant Targets
by
Ivan Nombela 1
, Marina Lopez-Lorigados 1, Maria Elizabeth Salvador-Mira 1, Sara Puente-Marin 1, Veronica Chico 1, Sergio Ciordia 2, Maria Carmen Mena 2, Luis Mercado 3, Julio Coll 4, Luis Perez 1 and Maria del Mar Ortega-Villaizan 1,*
Ivan Nombela 1, Marina Lopez-Lorigados 1, Maria Elizabeth Salvador-Mira 1, Sara Puente-Marin 1, Veronica Chico 1, Sergio Ciordia 2, Maria Carmen Mena 2, Luis Mercado 3, Julio Coll 4, Luis Perez 1 and Maria del Mar Ortega-Villaizan 1,*
1
Departamento de Bioquímica y Biología Molecular, Instituto de Biología Molecular y Celular (IBMC) and Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), Universidad Miguel Hernández (UMH), 03202 Elche, Spain
2
Unidad de Proteómica, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Madrid, Spain
3
Instituto de Biología, Pontificia Universidad Católica de Valparaíso (PUCV), Valparaíso 2373223, Chile
4
Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Vaccines 2019, 7(3), 63; https://doi.org/10.3390/vaccines7030063
Received: 5 June 2019 / Revised: 29 June 2019 / Accepted: 1 July 2019 / Published: 9 July 2019
Teleost red blood cells (RBCs) are nucleated and therefore can propagate cellular responses to exogenous stimuli. RBCs can mount an immune response against a variety of fish viruses, including the viral septicemia hemorrhagic virus (VHSV), which is one of the most prevalent fish viruses resulting in aquaculture losses. In this work, RBCs from blood and head kidney samples of rainbow trout challenged with VHSV were analyzed via transcriptomic and proteomic analyses. We detected an overrepresentation of differentially expressed genes (DEGs) related to the type I interferon response and signaling in RBCs from the head kidney and related to complement activation in RBCs from blood. Antigen processing and presentation of peptide antigen was overrepresented in RBCs from both tissues. DEGs shared by both tissues showed an opposite expression profile. In summary, this work has demonstrated that teleost RBCs can modulate the immune response during an in vivo viral infection, thus implicating RBCs as cell targets for the development of novel immunomodulants.
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Keywords:
erythrocytes; red blood cells; transcriptome; proteome; interferon; complement; VHSV; rhabdoviruses; Mx; IFIT5; β-defensin 1; antigen presentation
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MDPI and ACS Style
Nombela, I.; Lopez-Lorigados, M.; Salvador-Mira, M.E.; Puente-Marin, S.; Chico, V.; Ciordia, S.; Mena, M.C.; Mercado, L.; Coll, J.; Perez, L.; Ortega-Villaizan, M.M. Integrated Transcriptomic and Proteomic Analysis of Red Blood Cells from Rainbow Trout Challenged with VHSV Point Towards Novel Immunomodulant Targets. Vaccines 2019, 7, 63.
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