Next Article in Journal
AGS-v PLUS, a Mosquito Salivary Peptide Vaccine, Modulates the Response to Aedes Mosquito Bites in Humans
Previous Article in Journal
Immune Persistence Following a Single Dose of Varicella Vaccine: 5-Year and 8-Year Follow-Up of a Phase 3, Randomized, Double-Blind, Placebo-Controlled Trial
Previous Article in Special Issue
Degradation of Poliovirus Sabin 2 Genome After Electron Beam Irradiation
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Vaccines
Volume 13
Issue 10
10.3390/vaccines13101022
vaccines-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Generation of Polyclonal Antibodies Against Sabin Poliovirus D- and H-Antigens and Their Application in ELISA

by
Anna Zyrina
1,*,
Anna Shishova
1,2,*,
Irina Tcelykh
1,
Igor Levin
1,
Olga Shmeleva
1,
Nadezhda Borisenko
1,
Maya Ermakova
1,
Sergey Ivanov
1,
Anastasia Kovpak
1,
Vladislav Vasilenko
1,
Yuliya Rogova
1,
Alla Zhitkevich
1,
Nikita Khabibullin
1,
Yury Ivin
1,
Anastasia Piniaeva
1,
Alexandra Siniugina
1 and
Aydar Ishmukhametov
1,2
1
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences (Institute of Poliomyelitis), Moscow 108819, Russia
2
Institute for Translational Medicine and Biotechnology, First Moscow State Medical University (Sechenov University), Moscow 117418, Russia
*
Authors to whom correspondence should be addressed.
Vaccines 2025, 13(10), 1022; https://doi.org/10.3390/vaccines13101022
Submission received: 7 August 2025 / Revised: 18 September 2025 / Accepted: 29 September 2025 / Published: 30 September 2025
(This article belongs to the Special Issue Recent Scientific Development of Poliovirus Vaccines)
Versions Notes

Abstract

Background/Objectives: The World Health Organization (WHO) recommends the use of attenuated Sabin strains for the production of inactivated poliovirus vaccine (IPV), offering improved biosafety while retaining immunogenicity. To better characterize the antigenic composition of Sabin strain-based IPV (sIPV), including both the protective D-antigen and the non-protective H-antigen forms, we developed a method for purifying D- and H-antigens forms. Methods: D- and H-antigens of poliovirus Sabin strains types 1, 2, and 3 were purified using gradient ultracentrifugation and used to generate antigen-specific polyclonal antibodies. Results: The generated polyclonal antibodies demonstrated high specificity with neutralizing titers of antibodies against Sabin type 1 poliovirus—1:2048, against Sabin type 2 poliovirus—more than 1:2048, against Sabin type 3 poloivirus —1:2048. Conclusions: This antigen-specific antibody approach provides a valuable tool for routine quality control in sIPV manufacturing, enabling accurate quantification of immunogenic components and detection of potentially immunogenic degradation products during vaccine storage and distribution. Antibodies to the D-antigen allow assessment of immunogenic, neutralizing epitopes, while antibodies to the H-antigen provide a tool for detecting non-neutralizing components. This antigen-specific antibody approach offers a valuable tool for studying the antigenic structure of sIPV and for improving the accuracy of ELISA-based antigen quantification.
Keywords: Sabin poliovirus strains; rabbit polyclonal antibodies; ELISA test; D- and H-antigens; polio vaccines Sabin poliovirus strains; rabbit polyclonal antibodies; ELISA test; D- and H-antigens; polio vaccines

Share and Cite

MDPI and ACS Style

Zyrina, A.; Shishova, A.; Tcelykh, I.; Levin, I.; Shmeleva, O.; Borisenko, N.; Ermakova, M.; Ivanov, S.; Kovpak, A.; Vasilenko, V.; et al. Generation of Polyclonal Antibodies Against Sabin Poliovirus D- and H-Antigens and Their Application in ELISA. Vaccines 2025, 13, 1022. https://doi.org/10.3390/vaccines13101022

AMA Style

Zyrina A, Shishova A, Tcelykh I, Levin I, Shmeleva O, Borisenko N, Ermakova M, Ivanov S, Kovpak A, Vasilenko V, et al. Generation of Polyclonal Antibodies Against Sabin Poliovirus D- and H-Antigens and Their Application in ELISA. Vaccines. 2025; 13(10):1022. https://doi.org/10.3390/vaccines13101022

Chicago/Turabian Style

Zyrina, Anna, Anna Shishova, Irina Tcelykh, Igor Levin, Olga Shmeleva, Nadezhda Borisenko, Maya Ermakova, Sergey Ivanov, Anastasia Kovpak, Vladislav Vasilenko, and et al. 2025. "Generation of Polyclonal Antibodies Against Sabin Poliovirus D- and H-Antigens and Their Application in ELISA" Vaccines 13, no. 10: 1022. https://doi.org/10.3390/vaccines13101022

APA Style

Zyrina, A., Shishova, A., Tcelykh, I., Levin, I., Shmeleva, O., Borisenko, N., Ermakova, M., Ivanov, S., Kovpak, A., Vasilenko, V., Rogova, Y., Zhitkevich, A., Khabibullin, N., Ivin, Y., Piniaeva, A., Siniugina, A., & Ishmukhametov, A. (2025). Generation of Polyclonal Antibodies Against Sabin Poliovirus D- and H-Antigens and Their Application in ELISA. Vaccines, 13(10), 1022. https://doi.org/10.3390/vaccines13101022

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Article metric data becomes available approximately 24 hours after publication online.
Back to TopTop