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Article

Antitumoral Drug Potential of Tryptophan-Betaxanthin and Related Plant Betalains in the Caenorhabditis elegans Tumoral Model

Departamento de Bioquímica y Biología Molecular A, Unidad Docente de Biología, Facultad de Veterinaria, Regional Campus of International Excellence “Campus Mare Nostrum″, Universidad de Murcia, 30100 Murcia, Spain
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Antioxidants 2020, 9(8), 646; https://doi.org/10.3390/antiox9080646
Received: 1 June 2020 / Revised: 14 July 2020 / Accepted: 17 July 2020 / Published: 22 July 2020
(This article belongs to the Special Issue Plant Antioxidants in the “One Health” Paradigm)
Betalains are plants pigments identified as potent antioxidant molecules, naturally present in foods like beetroot and prickly pears. Although activities described for betalain-containing formulations include cancer prevention and treatment, the use of extracts instead of purified pigments has avoided the investigation of the real chemopreventive and chemotherapeutic potential of these phytochemicals. Three betalain-rich extracts and six individual pure betalains were used in this work to characterize the activity and to explore possible molecular mechanisms. The animal model Caenorhabditis elegans (tumoral strain JK1466) was used to evaluate the effect of betalains as chemotherapeutics drugs. An objective evaluation method of tumor growth in C. elegans has been developed to assess the possible antitumoral activity of the different treatments. This protocol allowed a fast and reliable screening of possible antitumoral drugs. Among the betalains tested, tryptophan-betaxanthin reduced tumor size by 56.4% and prolonged the animal’s lifespan by 9.3%, indicating high effectiveness and low toxicity. Structure–activity relationships are considered. Assays with mutant strains of C. elegans showed that the mechanism underlying these effects was the modulation of the DAF-16 transcription factor and the insulin signaling pathway. Our results indicate that tryptophan-betaxanthin and related betalains are strong candidates as antitumoral molecules in cancer treatment. View Full-Text
Keywords: Antitumoral; betalains; C. elegans; phytochemical; chemotherapy; tryptophan-betaxanthin Antitumoral; betalains; C. elegans; phytochemical; chemotherapy; tryptophan-betaxanthin
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MDPI and ACS Style

Henarejos-Escudero, P.; Hernández-García, S.; Guerrero-Rubio, M.A.; García-Carmona, F.; Gandía-Herrero, F. Antitumoral Drug Potential of Tryptophan-Betaxanthin and Related Plant Betalains in the Caenorhabditis elegans Tumoral Model. Antioxidants 2020, 9, 646. https://doi.org/10.3390/antiox9080646

AMA Style

Henarejos-Escudero P, Hernández-García S, Guerrero-Rubio MA, García-Carmona F, Gandía-Herrero F. Antitumoral Drug Potential of Tryptophan-Betaxanthin and Related Plant Betalains in the Caenorhabditis elegans Tumoral Model. Antioxidants. 2020; 9(8):646. https://doi.org/10.3390/antiox9080646

Chicago/Turabian Style

Henarejos-Escudero, Paula, Samanta Hernández-García, M. A. Guerrero-Rubio, Francisco García-Carmona, and Fernando Gandía-Herrero. 2020. "Antitumoral Drug Potential of Tryptophan-Betaxanthin and Related Plant Betalains in the Caenorhabditis elegans Tumoral Model" Antioxidants 9, no. 8: 646. https://doi.org/10.3390/antiox9080646

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