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Open AccessArticle

Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models

1
Department of Molecular Biology & Genetics, Democritus University of Thrace, 68100 Alexandroupolis, Greece
2
Laboratory of Histology and Embryology, School of Medicine, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, Greece
3
Department of Pharmacy, Division of Pharmacognosy & Natural Products Chemistry, University of Athens, 15771 Athens, Greece
4
Attiki-Pittas, Department of Quality Assurance, 15771 Athens, Greece
5
APIVITA SA, Industrial Park, Markopoulo, 19003 Athens, Greece
6
Department of Electron Microscopy & Molecular Pathology, The Cyprus Institute of Neurology & Genetics, Nicosia 2371, Cyprus
7
The Cyprus School of Molecular Medicine, Nicosia 1683, Cyprus
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(7), 566; https://doi.org/10.3390/antiox9070566 (registering DOI)
Received: 20 May 2020 / Revised: 17 June 2020 / Accepted: 23 June 2020 / Published: 30 June 2020
(This article belongs to the Special Issue Feature Papers in Antioxidants in 2020)
In the present study, we aimed to examine the antioxidant, antiaging and photoprotective properties of Greek honey samples of various botanical and geographical origin. Ethyl-acetate extracts were used and the and the total phenolic/flavonoid content and antioxidant capacity were evaluated. Honey extracts were then studied for their cytoprotective properties against UVB-induced photodamage using human immortalized keratinocytes (HaCaT) and/or reconstituted human skin tissue models. Specifically, the cytotoxicity, oxidative status, DNA damage and gene expression levels of specific matrix metalloproteinases (MMPs) were examined. Overall, the treatment of HaCaT cells with honey extracts resulted in lower levels of DNA strand breaks and attenuated the decrease in cell viability following UVB exposure. Additionally, honey extracts significantly decreased the total protein carbonyl content of the irradiated cells, however, they had no significant effect on their total antioxidant status. Finally, the extracts alleviated the UVB-induced up-regulation of MMPs-3, -7 and -9 in a model of reconstituted skin tissue. In conclusion, honey extracts exhibited significant photoprotective and antiaging properties under UVB exposure conditions and thus could be further exploited as promising agents for developing novel and naturally-based, antiaging cosmeceutical products. View Full-Text
Keywords: honey; antiaging; antioxidant; photoprotective; ultraviolet radiation (UV); DNA damage; matrix metalloproteinases (MMPs); HaCaT; 3D reconstituted skin model honey; antiaging; antioxidant; photoprotective; ultraviolet radiation (UV); DNA damage; matrix metalloproteinases (MMPs); HaCaT; 3D reconstituted skin model
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Karapetsas, A.; Voulgaridou, G.-P.; Iliadi, D.; Tsochantaridis, I.; Michail, P.; Kynigopoulos, S.; Lambropoulou, M.; Stavropoulou, M.-I.; Stathopoulou, K.; Karabournioti, S.; Aligiannis, N.; Gardikis, K.; Galanis, A.; Panayiotidis, M.I.; Pappa, A. Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models. Antioxidants 2020, 9, 566.

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