Next Article in Journal
Triple Combination of Ascorbate, Menadione and the Inhibition of Peroxiredoxin-1 Produces Synergistic Cytotoxic Effects in Triple-Negative Breast Cancer Cells
Next Article in Special Issue
Anthocyanins and Human Health—A Focus on Oxidative Stress, Inflammation and Disease
Previous Article in Journal
Transcriptome Analyses of lncRNAs in A2E-Stressed Retinal Epithelial Cells Unveil Advanced Links between Metabolic Impairments Related to Oxidative Stress and Retinitis Pigmentosa
Previous Article in Special Issue
Anti–Inflammatory and Antioxidant Properties of the Ethanol Extract of Clerodendrum Cyrtophyllum Turcz in Copper Sulfate-Induced Inflammation in Zebrafish
Open AccessArticle

Increasing the Magnesium Concentration in Various Dialysate Solutions Differentially Modulates Oxidative Stress in a Human Monocyte Cell Line

1
Departamento de Biología de Sistemas, Universidad de Alcalá, Alcalá de Henares, 28871 Madrid, Spain
2
Departamento Genética, Fisiología y Microbiología (Sección Fisiología), Universidad Complutense de Madrid, 28040 Madrid, Spain
3
Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 28041 Madrid, Spain
4
Sección de Nefrología, Hospital Universitario Infanta Leonor, 28031 Madrid, Spain
5
Departamento de Medicina, Universidad Complutense de Madrid, 2804 Madrid, Spain
6
Departamento de Biomedicina y Biotecnología, Universidad de Alcalá, Alcalá de Henares, 28805 Madrid, Spain
7
Instituto Ramón y Cajal de Investigación Sanitaria, (IRYCIS), 28034 Madrid, Spain
*
Authors to whom correspondence should be addressed.
These authors share senior authorship.
Antioxidants 2020, 9(4), 319; https://doi.org/10.3390/antiox9040319
Received: 27 February 2020 / Revised: 1 April 2020 / Accepted: 14 April 2020 / Published: 15 April 2020
(This article belongs to the Special Issue Antioxidants in the Prevention and Treatment of Chronic Diseases)
Oxidative stress is exacerbated in hemodialysis patients by several factors, including the uremic environment and the use of dialysis fluids (DFs). Since magnesium (Mg) plays a key role in modulating immune function and in reducing oxidative stress, we aimed to evaluate whether increasing the Mg concentration in different DFs could protect against oxidative stress in immunocompetent cells in vitro. Effect of ADF (acetate 3 mM), CDF (citrate 1 mM), and ACDF (citrate 0.8 mM + acetate 0.3 mM) dialysates with Mg at standard (0.5 mM) or higher (1, 1.25, and 2 mM) concentrations were assessed in THP-1 monocyte cultures. Reactive oxygen species (ROS) and malondialdehyde (MDA) levels were quantified under basal and uremic conditions (indoxyl sulfate (IS) treatment). Under uremic conditions, the three DFs with 0.5 mM Mg promoted higher ROS production and lipid damage than the control solution. However, CDF and ACDF induced lower levels of ROS and MDA, compared to that induced by ADF. High Mg concentration (1.25 and/or 2 mM) in CDF and ACDF protected against oxidative stress, indicated by reduced ROS and MDA levels compared to respective DFs with standard concentration of Mg. Increasing Mg concentrations in ADF promoted high ROS production and MDA content. Thus, an increase in Mg content in DFs has differential effects on the oxidative stress in IS-treated THP-1 cells depending on the dialysate used. View Full-Text
Keywords: chronic kidney disease; dialysates; lipid damage; magnesium; monocytes; oxidative stress; uremic toxins; reactive oxygen species chronic kidney disease; dialysates; lipid damage; magnesium; monocytes; oxidative stress; uremic toxins; reactive oxygen species
Show Figures

Graphical abstract

MDPI and ACS Style

Vida, C.; Carracedo, J.; de Sequera, P.; Bodega, G.; Pérez, R.; Alique, M.; Ramírez, R. Increasing the Magnesium Concentration in Various Dialysate Solutions Differentially Modulates Oxidative Stress in a Human Monocyte Cell Line. Antioxidants 2020, 9, 319.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop