Priming with γ-Aminobutyric Acid against Botrytis cinerea Reshuffles Metabolism and Reactive Oxygen Species: Dissecting Signalling and Metabolism

Janse van Rensburg, Henry Christopher; Van den Ende, Wim

doi:10.3390/antiox9121174

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Priming with γ-Aminobutyric Acid against Botrytis cinerea Reshuffles Metabolism and Reactive Oxygen Species: Dissecting Signalling and Metabolism

Henry Christopher Janse van Rensburg

and

Wim Van den Ende

Laboratory of Molecular Plant Biology, KU Leuven, Kasteelpark Arenberg 31, 3001 Leuven, Belgium

Author to whom correspondence should be addressed.

Antioxidants 2020, 9(12), 1174; https://doi.org/10.3390/antiox9121174

Received: 17 November 2020 / Revised: 21 November 2020 / Accepted: 23 November 2020 / Published: 25 November 2020

(This article belongs to the Special Issue Antioxidant Defenses in Plants)

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Abstract

The stress-inducible non-proteinogenic amino acid γ-aminobutyric acid (GABA) is known to alleviate several (a)biotic stresses in plants. GABA forms an important link between carbon and nitrogen metabolism and has been proposed as a signalling molecule in plants. Here, we set out to establish GABA as a priming compound against Botrytis cinerea in Arabidopsis thaliana and how metabolism and reactive oxygen species (ROS) are influenced after GABA treatment and infection. We show that GABA already primes disease resistance at low concentrations (100 µM), comparable to the well-characterized priming agent β-Aminobutyric acid (BABA). Treatment with GABA reduced ROS burst in response to flg22 (bacterial peptide derived from flagellum) and oligogalacturonides (OGs). Plants treated with GABA showed reduced H₂O₂ accumulation after infection due to increased activity of catalase and guaiacol peroxidase. Contrary to 100 µM GABA treatments, 1 mM exogenous GABA induced endogenous GABA before and after infection. Strikingly, 1 mM GABA promoted total and active nitrate reductase activity whereas 100 µM inhibited active nitrate reductase. Sucrose accumulated after GABA treatment, whereas glucose and fructose only accumulated in treated plants after infection. We propose that extracellular GABA signalling and endogenous metabolism can be separated at low exogenous concentrations. View Full-Text

Keywords: γ-aminobutyric acid (GABA); Botrytis cinerea; reactive oxygen species (ROS); sugars; priming; signalling

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Janse van Rensburg, H.C.; Van den Ende, W. Priming with γ-Aminobutyric Acid against Botrytis cinerea Reshuffles Metabolism and Reactive Oxygen Species: Dissecting Signalling and Metabolism. Antioxidants 2020, 9, 1174. https://doi.org/10.3390/antiox9121174

AMA Style

Janse van Rensburg HC, Van den Ende W. Priming with γ-Aminobutyric Acid against Botrytis cinerea Reshuffles Metabolism and Reactive Oxygen Species: Dissecting Signalling and Metabolism. Antioxidants. 2020; 9(12):1174. https://doi.org/10.3390/antiox9121174

Chicago/Turabian Style

Janse van Rensburg, Henry C., and Wim Van den Ende. 2020. "Priming with γ-Aminobutyric Acid against Botrytis cinerea Reshuffles Metabolism and Reactive Oxygen Species: Dissecting Signalling and Metabolism" Antioxidants 9, no. 12: 1174. https://doi.org/10.3390/antiox9121174

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Priming with γ-Aminobutyric Acid against Botrytis cinerea Reshuffles Metabolism and Reactive Oxygen Species: Dissecting Signalling and Metabolism

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