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Article

Microvesicle Formation Induced by Oxidative Stress in Human Erythrocytes

1
Center for Theoretical Problems of Physico-Chemical Pharmacology, Russian Academy of Sciences, Kosygina st., 4, 119991 Moscow, Russia
2
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr., 44, 194223 Saint-Petersburg, Russia
3
Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, 55131 Mainz, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Antioxidants 2020, 9(10), 929; https://doi.org/10.3390/antiox9100929
Received: 30 August 2020 / Revised: 24 September 2020 / Accepted: 25 September 2020 / Published: 28 September 2020
(This article belongs to the Special Issue Cellular Oxidative Stress)
Extracellular vesicles (EVs) released by different cell types play an important role in many physiological and pathophysiological processes. In physiological conditions, red blood cell (RBC)-derived EVs compose 4–8% of all circulating EVs, and oxidative stress (OS) as a consequence of different pathophysiological conditions significantly increases the amount of circulated RBC-derived EVs. However, the mechanisms of EV formation are not yet fully defined. To analyze OS-induced EV formation and RBC transformations, we used flow cytometry to evaluate cell esterase activity, caspase-3 activity, and band 3 clustering. Band 3 clustering was additionally analyzed by confocal microscopy. Two original laser diffraction-based approaches were used for the analysis of cell deformability and band 3 activity. Hemoglobin species were characterized spectrophotometrically. We showed that cell viability in tert-Butyl hydroperoxide-induced OS directly correlated with oxidant concentration to cell count ratio, and that RBC-derived EVs contained hemoglobin oxidized to hemichrome (HbChr). OS induced caspase-3 activation and band 3 clustering in cells and EVs. Importantly, we showed that OS-induced EV formation is independent of calcium. The presented data indicated that during OS, RBCs eliminated HbChr by vesiculation in order to sacrifice the cell itself, thereby prolonging lifespan and delaying the untimely clearance of in all other respects healthy RBCs. View Full-Text
Keywords: erythrocytes; microparticles; oxidative stress; vesiculation; band 3; tert-Bytyl hydroperoxide t-BOOH; nitric oxide donor; calcium ionophore A23187 erythrocytes; microparticles; oxidative stress; vesiculation; band 3; tert-Bytyl hydroperoxide t-BOOH; nitric oxide donor; calcium ionophore A23187
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MDPI and ACS Style

Sudnitsyna, J.; Skverchinskaya, E.; Dobrylko, I.; Nikitina, E.; Gambaryan, S.; Mindukshev, I. Microvesicle Formation Induced by Oxidative Stress in Human Erythrocytes. Antioxidants 2020, 9, 929. https://doi.org/10.3390/antiox9100929

AMA Style

Sudnitsyna J, Skverchinskaya E, Dobrylko I, Nikitina E, Gambaryan S, Mindukshev I. Microvesicle Formation Induced by Oxidative Stress in Human Erythrocytes. Antioxidants. 2020; 9(10):929. https://doi.org/10.3390/antiox9100929

Chicago/Turabian Style

Sudnitsyna, Julia, Elisaveta Skverchinskaya, Irina Dobrylko, Elena Nikitina, Stepan Gambaryan, and Igor Mindukshev. 2020. "Microvesicle Formation Induced by Oxidative Stress in Human Erythrocytes" Antioxidants 9, no. 10: 929. https://doi.org/10.3390/antiox9100929

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