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Open AccessArticle

Contribution of Individual Polyphenols to Antioxidant Activity of Cotoneaster bullatus and Cotoneaster zabelii Leaves—Structural Relationships, Synergy Effects and Application for Quality Control

1
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, 1 Muszynskiego, 90-151 Lodz, Poland
2
Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska, 90-236 Lodz, Poland
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(1), 69; https://doi.org/10.3390/antiox9010069
Received: 9 December 2019 / Revised: 4 January 2020 / Accepted: 9 January 2020 / Published: 12 January 2020
(This article belongs to the Special Issue Phenolic Profiling and Antioxidant Capacity in Plants)
Cotoneaster plants are sources of traditional medicines and dietary products, with health benefits resulting from their phenolic contents and antioxidant activity. In this work, active markers of the leaves of C. bullatus and C. zabelii were characterized and evaluated in an integrated phytochemical and biological activity study. Based on UHPLC-PDA-ESI-MS3 analysis, twelve analytes were preselected from the constituents of the hydromethanolic leaf extracts, and two of them—caffeoylmalic acid and quercetin 3--O-β-d-(2″--O-β-d-xylopyranosyl)galactopyranoside (QPH)—were isolated for full identification (NMR spectroscopy: 1H, 13C, COSY, HMBC, HMQC). All selected phenolics contributed to the antioxidant activity of the extracts, which was demonstrated in chemical in vitro tests (DPPH, FRAP, and TBARS) and in a biological model of human plasma exposed to oxidative/nitrative stress induced by peroxynitrite. This contribution was partly due to the synergy between individual polyphenols, evidenced by an isobolographic analysis of the interactions of (–)-epicatechin, chlorogenic acid, and QPH as representatives of three classes of Cotoneaster polyphenols. All twelve markers, including also neochlorogenic acid, cryptochlorogenic acid, procyanidin B2, procyanidin C1, rutin, hyperoside, isoquercitrin, and quercitrin, were thus applied as calibration standards, and a fast, accurate, reproducible, and fully validated RP-HPLC-PDA method for quality control and standardization of the target extracts was proposed. View Full-Text
Keywords: Cotoneaster; polyphenols; isolation; standardization; antioxidants; peroxynitrite; human plasma; synergy; HPLC-PDA; validation Cotoneaster; polyphenols; isolation; standardization; antioxidants; peroxynitrite; human plasma; synergy; HPLC-PDA; validation
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MDPI and ACS Style

Kicel, A.; Owczarek, A.; Kapusta, P.; Kolodziejczyk-Czepas, J.; Olszewska, M.A. Contribution of Individual Polyphenols to Antioxidant Activity of Cotoneaster bullatus and Cotoneaster zabelii Leaves—Structural Relationships, Synergy Effects and Application for Quality Control. Antioxidants 2020, 9, 69.

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