Ketogenic Diet and microRNAs Linked to Antioxidant Biochemical Homeostasis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Population
2.2. Immunoblot Analysis and RNAs Extraction
2.3. NanoString Sample Preparation and Data Analysis
2.4. In Silico Prediction of hsa-miR Target Genes
2.5. Statistical Analysis
3. Results
3.1. Characteristics of Subjects
3.2. Comparison of Obese, Lean and KD Array Profiles
3.3. In Silico Results
3.4. Validated hsa-miR Interaction and Western Blot Analysis of Catalase
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Characteristic | Obese (n = 14) | Lean (n = 17) | KD (n = 12) | p Value * |
---|---|---|---|---|
Age, y | 46.5 ± 10.51 | 46.83 ±12.32 | 46.6±11.56 | ns |
Height, cm | 175.1 ± 5.2 | 171.3 ± 6.6 | 176.3 ± 3.3 | ns |
Weight, kg | 107.5 ± 3.0 | 70.8 ± 3.8 | 96.97 ± 11.2 | <0.001 |
BMI, kg/m2 | 33.9 ± 1.2 | 22.1 ± 2.5 | 31.5 ± 1.3 | <0.001 |
Number of Target Genes | ||
---|---|---|
miRTargetLink Human | DIANA Tools | |
hsa-let-7b-5p | 124 | 312 |
hsa-let-7e-5p | 15 | 273 |
hsa-miR-143-3p | 32 | 82 |
hsa-miR-148b-3p | 10 | 218 |
hsa-miR-26a-5p | 52 | 391 |
hsa-miR-30a-5p | 119 | 458 |
hsa-miR-30e-5p | 7 | 412 |
hsa-miR-502-5p | 3 | 30 |
hsa-miR-504-5p | 6 | 7 |
hsa-miR-520h | 5 | 5 |
hsa-miR-548d-3p | 1 | 203 |
hsa-miR-590-5p | 2 | 43 |
hsa-miR-644a | 2 | 0 |
hsa-miR-877 | 0 | 19 |
Biochemical Pathways and Possible miRs Gene Interaction | ||
---|---|---|
miRNA | Validated target genes | |
Glutathione metabolism | hsa-let-7b-5p | GPX7, GSR, RRM2, GGCT |
has-let-7e-5p | GPX7 | |
hsa-miR-26a-5p | RRM2 | |
Chondroitin sulfate biosynthesis | hsa-let-7b-5p | CHPF2, XYLT2 |
Arachidonic acid metabolism | hsa-let-7b-5p | CYP2J2, GPX7, LTA4H, PTGS1, PTGS2, PTGES2 |
hsa-miR-26a-5p | PTGS1 | |
hsa-miR-143-3p | PTGS2 | |
Toll like receptor signalling pathway | hsa-let-7b-5p | IFNB1, NFKBIA, MAPK1, MAP2K2, TAB2 |
hsa-miR-26a-5p | IFNB1, IL6 | |
hsa-miR-30e-5p | CAT | |
hsa-miR-877-5p | MAPK8 | |
hsa-miR-148b-3p | PIK3CA, PIK3CG | |
hsa-miR-143-3p | AKT1 | |
hsa-miR-520h | TET3 | |
Natural killer cell mediated cytotoxicity and T Cell, B Cell receptor signalling pathways | hsa-let-7b-5p | IFNB1, NFATC1, NFATC3, NRAS, NFKBIA, PAK1, MAPK1, MAP2K2, PDK1, CD81 |
hsa-miR-26a-5p | IFNB1, SHC2, IL6 | |
hsa-miR-30e-5p | RELA, CAT | |
hsa-miR-504-5p | FAS | |
hsa-miR-877-5p | NFAT5, NRAS, PIK3CCA | |
hsa-miR-143-3p | HRAS, KRAS, AKT1 | |
hsa-miR-148a-3p | HLA-G, CCL28 | |
hsa-miR-548d-3p | AKT3, SOD2 |
Abbreviation | Gene Name |
---|---|
AKT1 | Serine-threonine protein kinase 1 |
AKT3 | Serine-threonine protein kinase 3 |
CAT | Catalase |
CCL28 | C-C motif chemokinine 28 precursor |
CD81 | CD81 antigen target proliferate antibody 1 |
CHPF2 | Chondroitin polymerizing factor 2 |
CYP2J2 | Cytochrome P450 2J2 |
FAS | FAS cell surface deat receptor |
GGCT | Gamma-glutamylcyclotransferase |
GPX7 | Glutathione peroxidase 7 |
GSR | Glutathione disulphide reductase |
HLA-G | HLA Class I Histocompatibility Antigen, Alpha Chain G |
HRAS | Hras protogoncogene GTPase |
IFNB1 | Interferon beta 1 |
IL6 | Interleukin-6 |
KRAS | Kras protogoncogene GTPase |
LTA4H | Leukotriene-A4 hydrolase |
MAP2K2 | Mitogen-activated protein kinase 2 |
MAPK1 | Mitogen-activated protein kinase 1 |
MAPK8 | Mitogen-activated protein kinase 8 |
NFAT5 | Nuclear factor of activated T-cells 5 |
NFATC1 | Nuclear factor of activated T cells 1 |
NFATC3 | Nuclear factor of activated T cells 3 |
NFKBIA | NFKB inhibitor alpha |
NRAS | NRAS-proto-oncogene |
PAK1 | Serine/threonine-protein kinase |
PDK1 | Phosphoinositide-dependent kinase-1 |
PIK3CA | Phosphaidylinositol-3-kinase |
PIK3CG | Phosphaidylinositol-4,5-Bisphosphatase 3-kinase |
PTGES2 | Prostaglandin-E synthase 2 |
PTGS1 | Prostaglandin-endoperoxidase synthase 1 |
PTGS2 | Prostaglandin-endoperoxdase synthase 2 |
PTES2 | Prostaglandin-E synthase 2 |
RELA | RELA-proto-oncogene |
RRM2 | Ribonucleotide reductase regulatory subunit M2 |
SHC2 | SHC-trasorming protein 2 |
TAB2 | TGF-beta activated kinase 1 binding protein 2 |
XYLT2 | Xylosyltransferase 2 |
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Cannataro, R.; Caroleo, M.C.; Fazio, A.; La Torre, C.; Plastina, P.; Gallelli, L.; Lauria, G.; Cione, E. Ketogenic Diet and microRNAs Linked to Antioxidant Biochemical Homeostasis. Antioxidants 2019, 8, 269. https://doi.org/10.3390/antiox8080269
Cannataro R, Caroleo MC, Fazio A, La Torre C, Plastina P, Gallelli L, Lauria G, Cione E. Ketogenic Diet and microRNAs Linked to Antioxidant Biochemical Homeostasis. Antioxidants. 2019; 8(8):269. https://doi.org/10.3390/antiox8080269
Chicago/Turabian StyleCannataro, Roberto, Maria Cristina Caroleo, Alessia Fazio, Chiara La Torre, Pierluigi Plastina, Luca Gallelli, Graziantonio Lauria, and Erika Cione. 2019. "Ketogenic Diet and microRNAs Linked to Antioxidant Biochemical Homeostasis" Antioxidants 8, no. 8: 269. https://doi.org/10.3390/antiox8080269