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Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats

Department of Ophthalmology, Faculty of Medicine, Shimane University, Shimane 693-0021, Japan
Department of Pathology, Uniformed Services University of the Health Science, Bethesda, MD 20814, USA
Department of Research and Development, Kotobuki Seika Co., Ltd., Tottori 683-0845, Japan
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(4), 79;
Received: 31 January 2019 / Revised: 18 March 2019 / Accepted: 25 March 2019 / Published: 27 March 2019
(This article belongs to the Special Issue Antioxidants and Retinal Disease)
We examined the cytoprotective effect of quercetin via activator protein (AP-1) and the heat shock protein 70 (Hsp70) pathway against light-induced retinal degeneration in rats. Quercetin was administered intraperitoneally to Sprague-Dawley rats for seven days before light exposure to intense white fluorescent light (3000 lux) for 24 h. Light-induced retinal damage was determined by the number of rows of photoreceptor cell nuclei, the microstructures of the rod outer segments and retinal pigment epithelium, and terminal deoxynucleotidyl transferase (TdT)-mediated 2′-Deoxyuridine-5′-triphosphate (dUTP) nick end labeling. To elucidate the cytoprotective mechanism of quercetin, expression levels were measured in the rat retinas of 8-hydroxy-deoxyguanosine (8-OHdG), a marker of oxidative stress; Hsp70; and transcription factor AP-1 transcription activity. Pretreatment with quercetin inhibited light-induced photoreceptor cellular apoptosis and subsequent retinal degeneration in rats. 8-OHdG and Hsp70 protein expressions were up-regulated markedly by light exposure and suppressed by quercetin pretreatment. The results of an electrophoretic mobility shift assay showed that AP-1-binding activity was activated by light exposure, and binding of c-Fos and c-Jun, but not JunB, mediated the binding activity. Intraperitoneal administration of quercetin decreases photooxidative damage in the retina and mediates cytoprotection against light-induced photoreceptor cell degeneration in rats. Suppression of the heterodimeric combination of c-Jun and c-Fos proteins at the AP-1 binding site is highly involved in quercetin-mediated cytoprotection. View Full-Text
Keywords: quercetin; light damage; oxidative stress; retina; AP-1 quercetin; light damage; oxidative stress; retina; AP-1
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MDPI and ACS Style

Koyama, Y.; Kaidzu, S.; Kim, Y.-C.; Matsuoka, Y.; Ishihara, T.; Ohira, A.; Tanito, M. Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats. Antioxidants 2019, 8, 79.

AMA Style

Koyama Y, Kaidzu S, Kim Y-C, Matsuoka Y, Ishihara T, Ohira A, Tanito M. Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats. Antioxidants. 2019; 8(4):79.

Chicago/Turabian Style

Koyama, Yasurou, Sachiko Kaidzu, Yong-Chul Kim, Yotaro Matsuoka, Tomoe Ishihara, Akihiro Ohira, and Masaki Tanito. 2019. "Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats" Antioxidants 8, no. 4: 79.

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