Next Article in Journal
Physico-Chemical Parameters, Phenolic Profile, In Vitro Antioxidant Activity and Volatile Compounds of Ladastacho (Lavandula stoechas) from the Region of Saidona
Next Article in Special Issue
Is There A Role for Abscisic Acid, A Proven Anti-Inflammatory Agent, in the Treatment of Ischemic Retinopathies?
Previous Article in Journal
Antioxidant Activities, Phenolic Profiles, and Organic Acid Contents of Fruit Vinegars
Previous Article in Special Issue
Progesterone, Lipoic Acid, and Sulforaphane as Promising Antioxidants for Retinal Diseases: A Review
Open AccessArticle

Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats

1
Department of Ophthalmology, Faculty of Medicine, Shimane University, Shimane 693-0021, Japan
2
Department of Pathology, Uniformed Services University of the Health Science, Bethesda, MD 20814, USA
3
Department of Research and Development, Kotobuki Seika Co., Ltd., Tottori 683-0845, Japan
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(4), 79; https://doi.org/10.3390/antiox8040079
Received: 31 January 2019 / Revised: 18 March 2019 / Accepted: 25 March 2019 / Published: 27 March 2019
(This article belongs to the Special Issue Antioxidants and Retinal Disease)
  |  
PDF [5780 KB, uploaded 12 April 2019]
  |  

Abstract

We examined the cytoprotective effect of quercetin via activator protein (AP-1) and the heat shock protein 70 (Hsp70) pathway against light-induced retinal degeneration in rats. Quercetin was administered intraperitoneally to Sprague-Dawley rats for seven days before light exposure to intense white fluorescent light (3000 lux) for 24 h. Light-induced retinal damage was determined by the number of rows of photoreceptor cell nuclei, the microstructures of the rod outer segments and retinal pigment epithelium, and terminal deoxynucleotidyl transferase (TdT)-mediated 2′-Deoxyuridine-5′-triphosphate (dUTP) nick end labeling. To elucidate the cytoprotective mechanism of quercetin, expression levels were measured in the rat retinas of 8-hydroxy-deoxyguanosine (8-OHdG), a marker of oxidative stress; Hsp70; and transcription factor AP-1 transcription activity. Pretreatment with quercetin inhibited light-induced photoreceptor cellular apoptosis and subsequent retinal degeneration in rats. 8-OHdG and Hsp70 protein expressions were up-regulated markedly by light exposure and suppressed by quercetin pretreatment. The results of an electrophoretic mobility shift assay showed that AP-1-binding activity was activated by light exposure, and binding of c-Fos and c-Jun, but not JunB, mediated the binding activity. Intraperitoneal administration of quercetin decreases photooxidative damage in the retina and mediates cytoprotection against light-induced photoreceptor cell degeneration in rats. Suppression of the heterodimeric combination of c-Jun and c-Fos proteins at the AP-1 binding site is highly involved in quercetin-mediated cytoprotection. View Full-Text
Keywords: quercetin; light damage; oxidative stress; retina; AP-1 quercetin; light damage; oxidative stress; retina; AP-1
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Koyama, Y.; Kaidzu, S.; Kim, Y.-C.; Matsuoka, Y.; Ishihara, T.; Ohira, A.; Tanito, M. Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats. Antioxidants 2019, 8, 79.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Antioxidants EISSN 2076-3921 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top