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Evaluation of the In Vitro Wound-Healing Activity of Calabrian Honeys

Department of Biotechnology, Chemistry and Pharmacy-Department of Excellence 2018-2022, University of Siena; Via Aldo Moro 2, 53100 Siena, Italy
Department of Pharmacy Health and Nutritional Sciences-Department of Excellence 2018-2022; University of Calabria; Edificio Polifunzionale, 87036 Arcavacata di Rende (CS), Italy
Department of Physical Sciences, Hearth and Environment; University of Siena; Via Laterina 8, 53100 Siena, Italy
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Antioxidants 2019, 8(2), 36;
Received: 13 December 2018 / Revised: 24 January 2019 / Accepted: 2 February 2019 / Published: 6 February 2019
(This article belongs to the Special Issue Dietary Polyphenols in Contemporary Disease)
PDF [4850 KB, uploaded 6 February 2019]


The healing of skin wounds and particularly chronic wounds, such as diabetic foot ulcers, is still a clinical emergency. Despite the many therapeutic tools that are available so far, none seems to be really effective and safe. In this context, we highlighted the renewed wound healing activity of honey, a viscous aromatic and sweet food, by way of in vitro wound-healing assays, using the HaCaT cell line. Specifically, we investigated five monofloral or multifloral honeys from different Calabrian provinces using them as such or extracted (by Amberlite® or n-hexane and ethyl acetate). The chemical composition of honeys was ascertained by 1H NMR spectroscopy and by the gas chromatography/mass spectrometry (GC/MS) method for volatile organic compounds (VOCs). Amongst the five tested honeys, BL1 and BL5 honeys showed the most promising healing properties. Pinocembrin, which was revealed in BL1 (multifloral) and BL5 (orange) honey samples, is a flavanol that is already known to possess interesting biological activities, including healing. This study aims to investigate how a traditional food such as honey, which is appreciated for its nutritional value and used in folk medicine, can be enhanced as an effective modern remedial to promote a multifaceted and safe healing activity for all skin wounds. View Full-Text
Keywords: wound healing; honey; diabetic foot ulcer; immunomodulation; HaCaT; Pinocembrin wound healing; honey; diabetic foot ulcer; immunomodulation; HaCaT; Pinocembrin

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Governa, P.; Carullo, G.; Biagi, M.; Rago, V.; Aiello, F. Evaluation of the In Vitro Wound-Healing Activity of Calabrian Honeys. Antioxidants 2019, 8, 36.

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