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Open AccessArticle

Bifunctional Chloroplastic DJ-1B from Arabidopsis thaliana is an Oxidation-Robust Holdase and a Glyoxalase Sensitive to H2O2

1
VIB-VUB Center for Structural Biology, VIB, 1050 Brussels, Belgium
2
Brussels Center for Redox Biology, 1050 Brussels, Belgium
3
Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium
4
Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium
5
VIB Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
6
de Duve Institute, Université Catholique de Louvain, 1200 Brussels, Belgium
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Antioxidants 2019, 8(1), 8; https://doi.org/10.3390/antiox8010008
Received: 25 November 2018 / Revised: 15 December 2018 / Accepted: 22 December 2018 / Published: 1 January 2019
(This article belongs to the Special Issue Oxidative Stress in Plant)
Members of the DJ-1 protein family are multifunctional enzymes whose loss increases the susceptibility of the cell to oxidative stress. However, little is known about the function of the plant DJ-1 homologs. Therefore, we analyzed the effect of oxidation on the structure and function of chloroplastic AtDJ-1B and studied the phenotype of T-DNA lines lacking the protein. In vitro oxidation of AtDJ-1B with H2O2 lowers its glyoxalase activity, but has no effect on its holdase chaperone function. Remarkably, upon oxidation, the thermostability of AtDJ-1B increases with no significant alteration of the overall secondary structure. Moreover, we found that AtDJ-1B transcript levels are invariable, and loss of AtDJ-1B does not affect plant viability, growth and stress response. All in all, two discrete functions of AtDJ-1B respond differently to H2O2, and AtDJ-1B is not essential for plant development under stress. View Full-Text
Keywords: chaperone; glyoxalase; holdase; redox chaperone; glyoxalase; holdase; redox
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Lewandowska, A.; Vo, T.N.; Nguyen, T.-D.H.; Wahni, K.; Vertommen, D.; Van Breusegem, F.; Young, D.; Messens, J. Bifunctional Chloroplastic DJ-1B from Arabidopsis thaliana is an Oxidation-Robust Holdase and a Glyoxalase Sensitive to H2O2. Antioxidants 2019, 8, 8.

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