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Open AccessArticle

Bifunctional Chloroplastic DJ-1B from Arabidopsis thaliana is an Oxidation-Robust Holdase and a Glyoxalase Sensitive to H2O2

VIB-VUB Center for Structural Biology, VIB, 1050 Brussels, Belgium
Brussels Center for Redox Biology, 1050 Brussels, Belgium
Structural Biology Brussels, Vrije Universiteit Brussel, 1050 Brussels, Belgium
Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium
VIB Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
de Duve Institute, Université Catholique de Louvain, 1200 Brussels, Belgium
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Antioxidants 2019, 8(1), 8;
Received: 25 November 2018 / Revised: 15 December 2018 / Accepted: 22 December 2018 / Published: 1 January 2019
(This article belongs to the Special Issue Oxidative Stress in Plant)
Members of the DJ-1 protein family are multifunctional enzymes whose loss increases the susceptibility of the cell to oxidative stress. However, little is known about the function of the plant DJ-1 homologs. Therefore, we analyzed the effect of oxidation on the structure and function of chloroplastic AtDJ-1B and studied the phenotype of T-DNA lines lacking the protein. In vitro oxidation of AtDJ-1B with H2O2 lowers its glyoxalase activity, but has no effect on its holdase chaperone function. Remarkably, upon oxidation, the thermostability of AtDJ-1B increases with no significant alteration of the overall secondary structure. Moreover, we found that AtDJ-1B transcript levels are invariable, and loss of AtDJ-1B does not affect plant viability, growth and stress response. All in all, two discrete functions of AtDJ-1B respond differently to H2O2, and AtDJ-1B is not essential for plant development under stress. View Full-Text
Keywords: chaperone; glyoxalase; holdase; redox chaperone; glyoxalase; holdase; redox
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Lewandowska, A.; Vo, T.N.; Nguyen, T.-D.H.; Wahni, K.; Vertommen, D.; Van Breusegem, F.; Young, D.; Messens, J. Bifunctional Chloroplastic DJ-1B from Arabidopsis thaliana is an Oxidation-Robust Holdase and a Glyoxalase Sensitive to H2O2. Antioxidants 2019, 8, 8.

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