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Anti-Oxidant and Tyrosinase Inhibitory In Vitro Activity of Amino Acids and Small Peptides: New Hints for the Multifaceted Treatment of Neurologic and Metabolic Disfunctions

Department of Pharmacy, G. d’Annunzio University of Chieti-Pescara, 66100 Chieti, Italy
Department of Biology, Science Faculty, Selcuk University, Konya 42130, Turkey
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(1), 7;
Received: 15 November 2018 / Revised: 14 December 2018 / Accepted: 22 December 2018 / Published: 26 December 2018
PDF [872 KB, uploaded 26 December 2018]


Oxidative damage is among the factors associated with the onset of chronic pathologies, such as neurodegenerative and metabolic diseases. Several classes of anti-oxidant compounds have been suggested as having a protective role against cellular stressors, but, in this perspective, peptides’ world represents a poorly explored source. In the present study, the free radical scavenging properties, the metal ion reducing power, and the metal chelating activity of a series of sulfurated amino acids and tripeptides were determined in vitro through canonical assays (DPPH, ABTS, CUPRAC, FRAP, PM, and EECC) and estimated in comparison with the corresponding activities of synthetic peptide semicarbazones, incorporating the peculiar non-proteinogenic amino acid, tert-leucine (tLeu). The compounds exhibited remarkable anti-oxidant properties. As expected, sulfurated compounds 15 were found to be the most efficient radical scavengers and strongest reductants. Nevertheless, tLeu-containing peptides 7 and 8 disclosed notable metal reducing and chelating activities. These unprecedented results indicate that tLeu-featuring di- and tripeptide backbones, bearing the semicarbazone chelating moiety, are compatible with the emergence of an anti-oxidant potential. Additionally, when tested against a panel of enzymes usually targeted for therapeutic purposes in neurodegenerative and metabolic disorders, all samples were found to be good inhibitors of tyrosinase. View Full-Text
Keywords: anti-oxidant; scavenger; neurodegenerative diseases; metabolic disorders anti-oxidant; scavenger; neurodegenerative diseases; metabolic disorders

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Luisi, G.; Stefanucci, A.; Zengin, G.; Dimmito, M.P.; Mollica, A. Anti-Oxidant and Tyrosinase Inhibitory In Vitro Activity of Amino Acids and Small Peptides: New Hints for the Multifaceted Treatment of Neurologic and Metabolic Disfunctions. Antioxidants 2019, 8, 7.

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