Next Article in Journal
Effects of N-Acetyl-Cysteine Supplementation through Drinking Water on the Glutathione Redox Status during the Weaning Transition of Piglets
Next Article in Special Issue
Medical and Dietary Uses of N-Acetylcysteine
Previous Article in Journal
Analysis of Popcorn (Zea Mays L. var. Everta) for Antioxidant Capacity and Total Phenolic Content
Previous Article in Special Issue
Potential Application of Prunus armeniaca L. and P. domestica L. Leaf Essential Oils as Antioxidant and of Cholinesterases Inhibitors
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle

The Influence of Ultrafiltration of Citrus limon L. Burm. cv Femminello Comune Juice on Its Chemical Composition and Antioxidant and Hypoglycemic Properties

1
Department of Pharmacy, Health Science and Nutrition, University of Calabria, Via Pietro Bucci, 87036 Arcavacata di Rende (CS), Italy
2
Department of Agricultural Science, Mediterranean University of Reggio Calabria, Via Graziella, Feo di Vito, 89123 Reggio Calabria, Italy
3
Department of Computer Engineering, Modelling, Electronics and System Science (DIMES), University of Calabria, Via Pietro Bucci, 87036 Arcavacata di Rende (CS), Italy
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(1), 23; https://doi.org/10.3390/antiox8010023
Received: 29 November 2018 / Revised: 9 January 2019 / Accepted: 12 January 2019 / Published: 16 January 2019
  |  
PDF [413 KB, uploaded 16 January 2019]
  |  

Abstract

Membrane separation has brought about a significant change in the food processing industry because it could operate separation at low temperature without a reduction of nutrients and bioactive compounds. Citrus limon L. Burm. cv Femminello comune juice, an Italian IGP (Protected Geographical Indication) product, was subjected to the ultrafiltation (UF) process using a cellulose acetate membrane, with a cut-off of 100 kDa, subjected to different transmembrane pressures (TMP, 05–1.5 bar). Untreated and ultra-filtrated (UF) juices were investigated for physicochemical parameters including pH, titratable acidity (TA), total soluble solids (TSS) and ascorbic acid content. Total phenols (TPC) and flavonoids (TFC) contents were also determined. Rutin, hesperidin, eriocitrin, and neohesperidin were selected as markers and quantified by HPLC. Antioxidant potential was investigated by using DPPH, ABTS, and FRAP tests. RACI was used to identify the sample with highest antioxidant potential. The hypoglycemic activity was examined using carbohydrates hydrolyzing enzymes assay. The application of increasing pressures across the membrane led to a reduction in TSS without causing a loss of bioactive compounds in terms of TPC and TFC. UF juice obtained with TMP of 1.5 bar (J3) showed a significant amount of eriocitrin and hesperidin with concentrations of 15.8 and 10.5 mg/100 mL, respectively. This sample showed the highest antioxidant potential and exhibited a promising α-amylase and α-glucosidase inhibitory activity with IC50 values of 31.1 and 35.3 mg/mL, respectively. Collectively our results support the use of cellulose acetate membrane to obtain an ultra-filtered juice with significant health potential. View Full-Text
Keywords: juice; membrane; flavonoids; biological properties; carbohydrates hydrolyzing enzymes juice; membrane; flavonoids; biological properties; carbohydrates hydrolyzing enzymes
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Loizzo, M.R.; Sicari, V.; Tundis, R.; Leporini, M.; Falco, T.; Calabrò, V. The Influence of Ultrafiltration of Citrus limon L. Burm. cv Femminello Comune Juice on Its Chemical Composition and Antioxidant and Hypoglycemic Properties. Antioxidants 2019, 8, 23.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Antioxidants EISSN 2076-3921 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top