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Open AccessArticle

Antiradical and Xanthine Oxidase Inhibitory Activity Evaluations of Averrhoa bilimbi L. Leaves and Tentative Identification of Bioactive Constituents through LC-QTOF-MS/MS and Molecular Docking Approach

1
Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University Malaysia, Pahang DM, Kuantan 25200, Malaysia
2
Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto P.M.B. 2346, Nigeria
3
Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA, Bandar Puncak Alam, Shah Alam 40450, Malaysia
4
Faculty of Pharmacy, Puncak Alam Campus, Universiti Teknologi MARA, Bandar Puncak Alam 42300, Malaysia
*
Authors to whom correspondence should be addressed.
Antioxidants 2018, 7(10), 137; https://doi.org/10.3390/antiox7100137
Received: 3 July 2018 / Revised: 21 September 2018 / Accepted: 25 September 2018 / Published: 8 October 2018
(This article belongs to the Special Issue Antioxidant Activity of Polyphenolic Plant Extracts)
The objective of the present study was to investigate the antiradical and xanthine oxidase inhibitory effects of Averrhoa bilimbi leaves. Hence, crude methanolic leaves extract and its resultant fractions, namely hexane, chloroform, and n-butanol were evaluated for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect and xanthine oxidase inhibitory activity. The active constituents were tentatively identified through LC-QTOF-MS/MS and molecular docking approaches. The n-butanol fraction of A. bilimbi crude methanolic leaves extract displayed significant DPPH radical scavenging effect with IC50 (4.14 ± 0.21 μg/mL) (p < 0.05), as well as xanthine oxidase inhibitory activity with IC50 (64.84 ± 3.93 μg/mL) (p < 0.05). Afzelechin 3-O-alpha-l-rhamnopyranoside and cucumerin A were tentatively identified as possible metabolites that contribute to the antioxidant activity of the n-butanol fraction. View Full-Text
Keywords: Averrhoa bilimbi; Oxalidaceae; DPPH; xanthine oxidase; LC-QTOF-MS/MS; molecular docking; tentative bioactive constituents Averrhoa bilimbi; Oxalidaceae; DPPH; xanthine oxidase; LC-QTOF-MS/MS; molecular docking; tentative bioactive constituents
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Ahmed, Q.U.; Alhassan, A.M.; Khatib, A.; Shah, S.A.A.; Hasan, M.M.; Sarian, M.N. Antiradical and Xanthine Oxidase Inhibitory Activity Evaluations of Averrhoa bilimbi L. Leaves and Tentative Identification of Bioactive Constituents through LC-QTOF-MS/MS and Molecular Docking Approach. Antioxidants 2018, 7, 137.

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