Phaeanthus vietnamensis Ban Ameliorates Lower Airway Inflammation in Experimental Asthmatic Mouse Model via Nrf2/HO-1 and MAPK Signaling Pathway
Abstract
:1. Introduction
2. Materials and Methods
2.1. Phaeanthus vietnamensis Extract Preparation
2.2. Chemical Constituents PVE Analyzed through UPLC-Q-TOF-MS/MS
2.3. Animal Protocol
2.4. Cytotoxicity MTT Assay
2.5. Rat Peritoneal Mast Cells (RPMCs) Degranulation Assay
2.6. Bronchoalveolar Lavage Fluid Analysis
2.7. Histopathology and Immunohistochemistry
2.8. Measurement of Immunoglobulins and Cytokine Using ELISA
2.9. Western Blot
2.10. Statistical Analysis
3. Results
3.1. Analyzed Compounds from Phaeanthus vietnamensis
3.2. PVE Is Non-Toxic to the Rat Peritoneal Mast Cell (RPMC)
3.3. PVE Prevented Degranulation of RPMCs by Compound 48/80
3.4. PVE Reduced the Recruitment of Inflammatory Cells in BALF of the OVA-Induced Asthmatic Mouse Model
3.5. PVE Reduced Asthma Histopathological Features in Lung Tissue of the OVA-Induced Asthmatic Mouse Model
3.6. PVE Reduced The Levels of OVA-Specific Antibodies and Histamine in the Serum of the OVA-Induced Asthmatic Mouse Model
3.7. PVE Restored the Balance of Helper T Cell Responses in the BALF of the OVA-Induced Asthmatic Mouse Model
3.8. Effect of PVE on Oxidative Stress in the OVA-Induced Asthmatic Mouse Model
3.9. PVE Inactivates the MAPK Signaling Pathway in the OVA-Induced Asthmatic Mouse Model
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Nguyen, T.V.; Vo, C.T.; Vo, V.M.; Nguyen, C.T.T.; Pham, T.M.; Piao, C.H.; Fan, Y.J.; Chai, O.H.; Bui, T.T. Phaeanthus vietnamensis Ban Ameliorates Lower Airway Inflammation in Experimental Asthmatic Mouse Model via Nrf2/HO-1 and MAPK Signaling Pathway. Antioxidants 2023, 12, 1301. https://doi.org/10.3390/antiox12061301
Nguyen TV, Vo CT, Vo VM, Nguyen CTT, Pham TM, Piao CH, Fan YJ, Chai OH, Bui TT. Phaeanthus vietnamensis Ban Ameliorates Lower Airway Inflammation in Experimental Asthmatic Mouse Model via Nrf2/HO-1 and MAPK Signaling Pathway. Antioxidants. 2023; 12(6):1301. https://doi.org/10.3390/antiox12061301
Chicago/Turabian StyleNguyen, Thi Van, Chau Tuan Vo, Van Minh Vo, Cong Thuy Tram Nguyen, Thi My Pham, Chun Hua Piao, Yan Jing Fan, Ok Hee Chai, and Thi Tho Bui. 2023. "Phaeanthus vietnamensis Ban Ameliorates Lower Airway Inflammation in Experimental Asthmatic Mouse Model via Nrf2/HO-1 and MAPK Signaling Pathway" Antioxidants 12, no. 6: 1301. https://doi.org/10.3390/antiox12061301