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Antioxidants
Volume 12
Issue 1
10.3390/antiox12010035
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Article

Glutathione during Post-Thaw Recovery Culture Can Mitigate Deleterious Impact of Vitrification on Bovine Oocytes

by
Lucia Olexiková
1,*,
Linda Dujíčková
1,2,
Alexander V. Makarevich
1,
Jiří Bezdíček
3,
Jana Sekaninová
4,
Andrea Nesvadbová
3 and
Peter Chrenek
1,5
1
Agricultural and Food Centre (NPPC), Research Institute for Animal Production Nitra, Hlohovecká 2, 95141 Lužianky, Slovakia
2
Department of Botany and Genetics, Constantine the Philosopher University Nitra, Tr. A. Hlinku 1, 94974 Nitra, Slovakia
3
Department of Zoology, Faculty of Science, Palacký University Olomouc, 17. Listopadu 50, 77900 Olomouc, Czech Republic
4
Department of Biochemistry, Faculty of Science, Palacký University Olomouc, Šlechtitelů 27, 77900 Olomouc, Czech Republic
5
Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 Nitra, Slovakia
*
Author to whom correspondence should be addressed.
Antioxidants 2023, 12(1), 35; https://doi.org/10.3390/antiox12010035
Submission received: 25 November 2022 / Revised: 16 December 2022 / Accepted: 20 December 2022 / Published: 24 December 2022
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Abstract

Vitrification of bovine oocytes can impair subsequent embryo development mostly due to elevated oxidative stress. This study was aimed at examining whether glutathione, a known antioxidant, can improve further embryo development when added to devitrified oocytes for a short recovery period. Bovine in vitro matured oocytes were vitrified using an ultra-rapid cooling technique on electron microscopy grids. Following warming, the oocytes were incubated in the recovery medium containing glutathione (0, 1.5, or 5 mmol L−1) for 3 h (post-warm recovery). Afterwards, the oocytes were lysed for measuring the total antioxidant capacity (TAC), activity of peroxidase, catalase and glutathione reductase, and ROS formation. The impact of vitrification on mitochondrial and lysosomal activities was also examined. Since glutathione, added at 5 mmol L−1, significantly increased the TAC of warmed oocytes, in the next set of experiments this dose was applied for post–warm recovery of oocytes used for IVF. Glutathione in the recovery culture did not change the total blastocyst rate, while increased the proportion of faster developing blastocysts (Day 6–7), reduced the apoptotic cell ratio and reversed the harmful impact of vitrification on the actin cytoskeleton. These results suggest that even a short recovery culture with antioxidant(s) can improve the development of bovine devitrified oocytes.
Keywords: bovine oocyte; vitrification; glutathione bovine oocyte; vitrification; glutathione

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MDPI and ACS Style

Olexiková, L.; Dujíčková, L.; Makarevich, A.V.; Bezdíček, J.; Sekaninová, J.; Nesvadbová, A.; Chrenek, P. Glutathione during Post-Thaw Recovery Culture Can Mitigate Deleterious Impact of Vitrification on Bovine Oocytes. Antioxidants 2023, 12, 35. https://doi.org/10.3390/antiox12010035

AMA Style

Olexiková L, Dujíčková L, Makarevich AV, Bezdíček J, Sekaninová J, Nesvadbová A, Chrenek P. Glutathione during Post-Thaw Recovery Culture Can Mitigate Deleterious Impact of Vitrification on Bovine Oocytes. Antioxidants. 2023; 12(1):35. https://doi.org/10.3390/antiox12010035

Chicago/Turabian Style

Olexiková, Lucia, Linda Dujíčková, Alexander V. Makarevich, Jiří Bezdíček, Jana Sekaninová, Andrea Nesvadbová, and Peter Chrenek. 2023. "Glutathione during Post-Thaw Recovery Culture Can Mitigate Deleterious Impact of Vitrification on Bovine Oocytes" Antioxidants 12, no. 1: 35. https://doi.org/10.3390/antiox12010035

APA Style

Olexiková, L., Dujíčková, L., Makarevich, A. V., Bezdíček, J., Sekaninová, J., Nesvadbová, A., & Chrenek, P. (2023). Glutathione during Post-Thaw Recovery Culture Can Mitigate Deleterious Impact of Vitrification on Bovine Oocytes. Antioxidants, 12(1), 35. https://doi.org/10.3390/antiox12010035

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