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Antioxidants
Volume 11
Issue 3
10.3390/antiox11030528
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Article

Technical Aspects of Coenzyme Q10 Analysis: Validation of a New HPLC-ED Method

by
Abraham J. Paredes-Fuentes
1,2,*,
Clara Oliva
2,
Raquel Montero
2,
Patricia Alcaide
3,4,
George J. G. Ruijter
5,
Judit García-Villoria
1,4,6,
Pedro Ruiz-Sala
3,4 and
Rafael Artuch
2,4
1
Division of Inborn Errors of Metabolism-IBC, Biochemistry and Molecular Genetics Department, Hospital Clínic de Barcelona, 08028 Barcelona, Spain
2
Clinical Biochemistry Department, Institut de Recerca Sant Joan de Déu, Hospital Sant Joan de Déu, Esplugues de Llobregat, 08950 Barcelona, Spain
3
Centro de Diagnóstico de Enfermedades Moleculares, Universidad Autónoma Madrid, IdiPAZ, 28049 Madrid, Spain
4
Biomedical Network Research Centre on Rare Diseases (CIBERER), Instituto de Salud Carlos III, 28029 Madrid, Spain
5
Department of Clinical Genetics, Center for Lysosomal and Metabolic Diseases, Erasmus University Medical Center, 3015 GD Rotterdam, The Netherlands
6
August Pi i Sunyer Biomedical Research Institute (IDIBAPS), 08036 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Antioxidants 2022, 11(3), 528; https://doi.org/10.3390/antiox11030528
Submission received: 24 January 2022 / Revised: 23 February 2022 / Accepted: 8 March 2022 / Published: 10 March 2022
(This article belongs to the Special Issue Challenges of CoQ10 Deficiency in Diagnostic, New Models of Disease and Therapy)
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Abstract

The biochemical measurement of the CoQ status in different tissues can be performed using HPLC with electrochemical detection (ED). Because the production of the electrochemical cells used with the Coulochem series detectors was discontinued, we aimed to standardize a new HPLC-ED method with new equipment. We report all technical aspects, troubleshooting and its performance in different biological samples, including plasma, skeletal muscle homogenates, urine and cultured skin fibroblasts. Analytical variables (intra- and inter-assay precision, linearity, analytical measurement range, limit of quantification, limit of detection and accuracy) were validated in calibrators and plasma samples and displayed adequate results. The comparison of the results of a new ERNDIM external quality control (EQC) scheme for the plasma CoQ determination between HPLC-ED (Lab 1) and LC-MS/MS (Lab 2) methods shows that the results of the latter were slightly higher in most cases, although a good consistency was generally observed. In conclusion, the new method reported here showed a good analytical performance. The global quality of the EQC scheme results among different participants can be improved with the contribution of more laboratories.
Keywords: coenzyme Q10; HPLC with electrochemical detection; electrochemical cells; external quality control; ERNDIM coenzyme Q10; HPLC with electrochemical detection; electrochemical cells; external quality control; ERNDIM

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MDPI and ACS Style

Paredes-Fuentes, A.J.; Oliva, C.; Montero, R.; Alcaide, P.; Ruijter, G.J.G.; García-Villoria, J.; Ruiz-Sala, P.; Artuch, R. Technical Aspects of Coenzyme Q10 Analysis: Validation of a New HPLC-ED Method. Antioxidants 2022, 11, 528. https://doi.org/10.3390/antiox11030528

AMA Style

Paredes-Fuentes AJ, Oliva C, Montero R, Alcaide P, Ruijter GJG, García-Villoria J, Ruiz-Sala P, Artuch R. Technical Aspects of Coenzyme Q10 Analysis: Validation of a New HPLC-ED Method. Antioxidants. 2022; 11(3):528. https://doi.org/10.3390/antiox11030528

Chicago/Turabian Style

Paredes-Fuentes, Abraham J., Clara Oliva, Raquel Montero, Patricia Alcaide, George J. G. Ruijter, Judit García-Villoria, Pedro Ruiz-Sala, and Rafael Artuch. 2022. "Technical Aspects of Coenzyme Q10 Analysis: Validation of a New HPLC-ED Method" Antioxidants 11, no. 3: 528. https://doi.org/10.3390/antiox11030528

APA Style

Paredes-Fuentes, A. J., Oliva, C., Montero, R., Alcaide, P., Ruijter, G. J. G., García-Villoria, J., Ruiz-Sala, P., & Artuch, R. (2022). Technical Aspects of Coenzyme Q10 Analysis: Validation of a New HPLC-ED Method. Antioxidants, 11(3), 528. https://doi.org/10.3390/antiox11030528

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