Maternal Calorie Restriction Induces a Transcriptional Cytoprotective Response in Embryonic Liver Partially Dependent on Nrf2
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mouse Model
2.2. In Utero Calorie Restriction
2.3. RNA Preparation and Quantitative Real-Time PCR
2.4. OS Assessment
2.5. Statistics
3. Results
3.1. Maternal Calorie Restriction Induces an Antioxidant/Cytoprotective Transcriptional Response in Fetal Liver
3.2. Calorie Restriction Effects on Gluconeogenic and Lipogenic Gene Expression in Fetal Liver
3.3. No Remarkable Difference in OS Was Found: Neither in the Livers of Embryos, Nor in the Livers of Their Mothers That had been Exposed to Calorie Restriction
3.4. Lack of Nrf2 Partially Attenuates the Antioxidant Transcriptional Response to Maternal Calorie Restriction in Murine Fetal Livers
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene (NCBI ID) | Forward Primer | Reverse Primer |
---|---|---|
Acacb (100705) | CCTTTGGCAACAAGCAAGGTA | AGTCGTACACATAGGTGGTCC |
Atf4 (11911) | CCTGAACAGCGAAGTGTTGG | TGGAGAACCCATGAGGTTTCAA |
Chac1 (69065) | CTGTGGATTTTCGGGTACGG | CCCCTATGGAAGGTGTCTCC |
Ddit3 (13198) | AAGCCTGGTATGAGGATCTGC | TTCCTGGGGATGAGATATAGGTG |
Fasn (14104) | GGAGGTGGTGATAGCCGGTAT | TGGGTAATCCATAGAGCCCAG |
Gsta1 (14857) | AAGCCCGTGCTTCACTACTTC | GGGCACTTGGTCAAACATCAAA |
Gsta4 (14860) | TGATTGCCGTGGCTCCATTTA | CAACGAGAAAAGCCTCTCCGT |
G6pase (14377) | CTAGCTTTGATCTGGTTGTCAG | GTTGAACCAGTCTCCGACCA |
Nqo1 (18104) | CATTCTGAAAGGCTGGTTTGA | CTAGCTTTGATCTGGTTGTCAG |
Pck1 (18534) | CTGCATAACGGTCTGGACTTC | CAGCAACTGCCCGTACTCC |
Rps29 (20090) | TCTACTGGAGTCACCCACGGAA | GGAAGCACTGGCGGCACA |
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Habeos, G.I.; Filippopoulou, F.; Habeos, E.E.; Kalaitzopoulou, E.; Skipitari, M.; Papadea, P.; Lagoumintzis, G.; Niarchos, A.; Georgiou, C.D.; Chartoumpekis, D.V. Maternal Calorie Restriction Induces a Transcriptional Cytoprotective Response in Embryonic Liver Partially Dependent on Nrf2. Antioxidants 2022, 11, 2274. https://doi.org/10.3390/antiox11112274
Habeos GI, Filippopoulou F, Habeos EE, Kalaitzopoulou E, Skipitari M, Papadea P, Lagoumintzis G, Niarchos A, Georgiou CD, Chartoumpekis DV. Maternal Calorie Restriction Induces a Transcriptional Cytoprotective Response in Embryonic Liver Partially Dependent on Nrf2. Antioxidants. 2022; 11(11):2274. https://doi.org/10.3390/antiox11112274
Chicago/Turabian StyleHabeos, George I., Fotini Filippopoulou, Evagelia E. Habeos, Electra Kalaitzopoulou, Marianna Skipitari, Polyxeni Papadea, George Lagoumintzis, Athanasios Niarchos, Christos D. Georgiou, and Dionysios V. Chartoumpekis. 2022. "Maternal Calorie Restriction Induces a Transcriptional Cytoprotective Response in Embryonic Liver Partially Dependent on Nrf2" Antioxidants 11, no. 11: 2274. https://doi.org/10.3390/antiox11112274