Caesalpinia sappan Linn. Ameliorates Allergic Nasal Inflammation by Upregulating the Keap1/Nrf2/HO-1 Pathway in an Allergic Rhinitis Mouse Model and Nasal Epithelial Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation and Chemical Profiling of CSLW
2.2. Animals
2.3. OVA-Induced AR Mouse Model and Treatment
2.4. Determination of Nasal Symptoms
2.5. Nasal Lavage Fluid (NALF) Collection and Cell Counting
2.6. Serum Collection and Measurement of Serum OVA-Specific IgE, Histamine, IL-5, and IL-13 Levels
2.7. Histopathological Analysis of Nasal Tissues
2.8. Immunohistochemical Analysis of Nasal Tissues
2.9. Cell Culture
2.10. Cell Viability
2.11. Measurement of Eotaxin-3, Periostin, and MUC5AC Levels
2.12. Immunofluorescence Staining
2.13. Determination of Protein Levels
2.14. Statistical Analysis
3. Results
3.1. CSLW Alleviates Nasal Allergy Symptoms and Reduces Immune Cell Accumulation of NALF in OVA-Induced AR Mice
3.2. CSLW Inhibits OVA-Specific IgE, Histamine, and Th2 Inflammatory Cytokine Levels in OVA-Induced AR Mice
3.3. CSLW Inhibits Nasal Mucosa Thickness and Goblet Cell Levels in the Nasal Tissues of OVA-Induced AR Mice
3.4. CSLW Inhibits Eosinophil Infiltration, Periostin and MUC5AC Levels, and Oxidative Damage of Nasal Tissue in OVA-Induced AR Mice
3.5. CSLW Inhibits Eotaxin-3, MUC5AC, and Periostin Expression by Reducing IL-4/IL-13-Induced ROS Production in HNEpCs
3.6. CSLW Inhibits the Production of IL-4/IL-13-Induced Inflammatory Mediators in HNEpCs via Regulation of the ERK-MAPK and Nrf2/HO-1 Signaling Pathways
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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No. | Rt (min) | Calculated (m/z) | Estimated (m/z) | Error (ppm) | Adduct | Formula | MS2 Fragments (m/z) | Identification |
---|---|---|---|---|---|---|---|---|
1 | 5.26 | 303.0874 | 303.0874 | −0.0253 | [M − H]− | C16H16O6 | 123, 163, 229 | Episappanol |
2 | 5.61 | 303.0874 | 303.0874 | −0.0253 | [M − H]− | C16H16O6 | 231 | Protosappanin B * |
3 | 6.02 | 303.0874 | 303.0874 | −0.0253 | [M − H]− | C16H16O6 | 229, 257 | Sappanol |
4 | 5.7 | 285.0768 | 285.0768 | −0.0236 | [M − H]− | C16H14O5 | 163 | Brazilin |
5 | 8.9 | 285.0768 | 285.077 | −0.3214 | [M − H]− | C16H14O5 | 123, 165 | 3-deoxysappanone B |
6 | 7.1 | 283.0612 | 283.0612 | 0.1723 | [M − H]− | C16H12O5 | 240, 173 | Brazilein |
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Pyun, B.-J.; Jo, K.; Lee, J.Y.; Lee, A.; Jung, M.-A.; Hwang, Y.-H.; Jung, D.H.; Ji, K.-Y.; Choi, S.; Kim, Y.H.; et al. Caesalpinia sappan Linn. Ameliorates Allergic Nasal Inflammation by Upregulating the Keap1/Nrf2/HO-1 Pathway in an Allergic Rhinitis Mouse Model and Nasal Epithelial Cells. Antioxidants 2022, 11, 2256. https://doi.org/10.3390/antiox11112256
Pyun B-J, Jo K, Lee JY, Lee A, Jung M-A, Hwang Y-H, Jung DH, Ji K-Y, Choi S, Kim YH, et al. Caesalpinia sappan Linn. Ameliorates Allergic Nasal Inflammation by Upregulating the Keap1/Nrf2/HO-1 Pathway in an Allergic Rhinitis Mouse Model and Nasal Epithelial Cells. Antioxidants. 2022; 11(11):2256. https://doi.org/10.3390/antiox11112256
Chicago/Turabian StylePyun, Bo-Jeong, Kyuhyung Jo, Joo Young Lee, Ami Lee, Myung-A Jung, Youn-Hwan Hwang, Dong Ho Jung, Kon-Young Ji, Susanna Choi, Yun Hee Kim, and et al. 2022. "Caesalpinia sappan Linn. Ameliorates Allergic Nasal Inflammation by Upregulating the Keap1/Nrf2/HO-1 Pathway in an Allergic Rhinitis Mouse Model and Nasal Epithelial Cells" Antioxidants 11, no. 11: 2256. https://doi.org/10.3390/antiox11112256